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MX2018010575A - Amplificación automatizada por recombinasa-polimerasa anidada. - Google Patents

Amplificación automatizada por recombinasa-polimerasa anidada.

Info

Publication number
MX2018010575A
MX2018010575A MX2018010575A MX2018010575A MX2018010575A MX 2018010575 A MX2018010575 A MX 2018010575A MX 2018010575 A MX2018010575 A MX 2018010575A MX 2018010575 A MX2018010575 A MX 2018010575A MX 2018010575 A MX2018010575 A MX 2018010575A
Authority
MX
Mexico
Prior art keywords
amplification
sample
product
phase
recombinase polymerase
Prior art date
Application number
MX2018010575A
Other languages
English (en)
Inventor
John Whyte Murray
A Armes Niall
Piepenburg Olaf
Nentwich Oliver
Jean Greenwood Catherine
Original Assignee
Alere San Diego Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alere San Diego Inc filed Critical Alere San Diego Inc
Publication of MX2018010575A publication Critical patent/MX2018010575A/es

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
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    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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    • B01L2300/06—Auxiliary integrated devices, integrated components
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

Un sistema de pruebas para la gripe que incluye un módulo para muestras, un dispositivo de microfluidos de amplificación de ácidos nucleicos y un analizador para facilitar una total amplificación automatizada por recombinasa-polimerasa anidada (RPA, por sus siglas en inglés) en una muestra suministrada en el dispositivo de amplificación de ácidos nucleicos mediante el módulo para muestras. El sistema de pruebas incluye suministrar una muestra en un dispositivo de microfluidos y amplificar una secuencia específica de polinucleótidos en la muestra. La amplificación de la secuencia específica de polinucleótidos incluye llevar a cabo una primera fase de amplificación de la muestra para generar un producto de la primera amplificación y realizar una segunda fase de amplificación en el producto de la primera amplificación para crear un producto de una segunda amplificación. El producto de la segunda amplificación incluye una secuencia más pequeña completamente contenida en el producto de la primera amplificación generado durante la primera fase de amplificación.
MX2018010575A 2016-03-04 2017-03-03 Amplificación automatizada por recombinasa-polimerasa anidada. MX2018010575A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662303934P 2016-03-04 2016-03-04
PCT/US2017/020782 WO2017152122A2 (en) 2016-03-04 2017-03-03 Automated nested recombinase polymerase amplification

Publications (1)

Publication Number Publication Date
MX2018010575A true MX2018010575A (es) 2019-01-10

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ID=59744470

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MX2018010575A MX2018010575A (es) 2016-03-04 2017-03-03 Amplificación automatizada por recombinasa-polimerasa anidada.

Country Status (11)

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US (1) US12195813B2 (es)
EP (2) EP3423599B1 (es)
JP (3) JP2019508043A (es)
KR (2) KR20220167397A (es)
CN (1) CN109072289A (es)
AU (1) AU2017228401B2 (es)
BR (1) BR112018067739A2 (es)
CA (1) CA3016582A1 (es)
ES (1) ES2950618T3 (es)
MX (1) MX2018010575A (es)
WO (1) WO2017152122A2 (es)

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KR102473905B1 (ko) 2022-12-06
EP4275794A2 (en) 2023-11-15
CN109072289A (zh) 2018-12-21
ES2950618T3 (es) 2023-10-11
KR20220167397A (ko) 2022-12-20
US12195813B2 (en) 2025-01-14
JP2021118695A (ja) 2021-08-12
BR112018067739A2 (pt) 2019-01-08
AU2017228401B2 (en) 2023-07-27
JP2023123447A (ja) 2023-09-05
US20200232050A1 (en) 2020-07-23
EP3423599B1 (en) 2023-04-19
EP4275794A3 (en) 2024-01-17
AU2017228401A1 (en) 2018-09-27
JP2019508043A (ja) 2019-03-28
WO2017152122A3 (en) 2017-10-19
WO2017152122A2 (en) 2017-09-08
KR20180133406A (ko) 2018-12-14
CA3016582A1 (en) 2017-09-08
EP3423599A4 (en) 2020-01-08
EP3423599A2 (en) 2019-01-09

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