[go: up one dir, main page]

MX2018006814A - Metodos para la modificacion genetica de plantas. - Google Patents

Metodos para la modificacion genetica de plantas.

Info

Publication number
MX2018006814A
MX2018006814A MX2018006814A MX2018006814A MX2018006814A MX 2018006814 A MX2018006814 A MX 2018006814A MX 2018006814 A MX2018006814 A MX 2018006814A MX 2018006814 A MX2018006814 A MX 2018006814A MX 2018006814 A MX2018006814 A MX 2018006814A
Authority
MX
Mexico
Prior art keywords
methods
plant
plants
genetic modification
expression
Prior art date
Application number
MX2018006814A
Other languages
English (en)
Inventor
Hamilton Richard
Liang Delin
I Pennell Roger
Original Assignee
Ceres Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ceres Inc filed Critical Ceres Inc
Publication of MX2018006814A publication Critical patent/MX2018006814A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01012—Cellulose synthase (UDP-forming) (2.4.1.12)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)

Abstract

Se describen métodos y materiales para la modificación genética de plantas mediante el direccionamiento génico específico y edición precisa de secuencias de ácidos nucleicos en una planta; los métodos y materiales provistos en la presente permiten a uno editar el genoma de planta por diseño para controlar la expresión de genes endógenos y/o controlar la transmisión y expresión de rasgos transgénicos; también se proveen métodos para producir plantas que tienen un rasgo agronómico aconsejable al cruzar una planta transgénica que expresa un ARNg con una planta que expresa una enzima Cas, y seleccionar una planta de progenie que tiene el rasgo agronómico aconsejable o una semilla de la misma.
MX2018006814A 2015-12-02 2016-12-02 Metodos para la modificacion genetica de plantas. MX2018006814A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562261944P 2015-12-02 2015-12-02
PCT/US2016/064733 WO2017096237A1 (en) 2015-12-02 2016-12-02 Methods for genetic modification of plants

Publications (1)

Publication Number Publication Date
MX2018006814A true MX2018006814A (es) 2019-08-05

Family

ID=58797902

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018006814A MX2018006814A (es) 2015-12-02 2016-12-02 Metodos para la modificacion genetica de plantas.

Country Status (6)

Country Link
US (2) US11118189B2 (es)
EP (1) EP3383168A4 (es)
BR (1) BR112018011242B1 (es)
CA (1) CA3007288A1 (es)
MX (1) MX2018006814A (es)
WO (1) WO2017096237A1 (es)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
US12043852B2 (en) 2015-10-23 2024-07-23 President And Fellows Of Harvard College Evolved Cas9 proteins for gene editing
EP3383168A4 (en) 2015-12-02 2019-05-08 Ceres, Inc. METHOD FOR THE GENETIC MODIFICATION OF PLANTS
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
CN109804066A (zh) 2016-08-09 2019-05-24 哈佛大学的校长及成员们 可编程cas9-重组酶融合蛋白及其用途
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
AU2017342543B2 (en) 2016-10-14 2024-06-27 President And Fellows Of Harvard College AAV delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3592381A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Cancer vaccine
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
KR20190127797A (ko) 2017-03-10 2019-11-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 시토신에서 구아닌으로의 염기 편집제
CA3057192A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
WO2018237369A2 (en) * 2017-06-23 2018-12-27 Vical Incorporated Lipid nanoparticle (lnp)-mediated delivery of a crispr-expressing plasmid dna for treating chronic hepatitis b virus infection
WO2019014917A1 (zh) * 2017-07-21 2019-01-24 中国科学院遗传与发育生物学研究所 一种基因编辑系统及应用其对植物基因组进行编辑的方法
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
CA3082251A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors
EP3724214A4 (en) 2017-12-15 2021-09-01 The Broad Institute Inc. SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
EP3521436A1 (en) * 2018-06-27 2019-08-07 VIB vzw Complex breeding in plants
US12281338B2 (en) 2018-10-29 2025-04-22 The Broad Institute, Inc. Nucleobase editors comprising GeoCas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
AU2020228277B9 (en) * 2019-02-25 2025-10-23 Syngenta Crop Protection Ag Compositions and methods for driving T1 event diversity
DE112020001306T5 (de) 2019-03-19 2022-01-27 Massachusetts Institute Of Technology Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021183874A1 (en) * 2020-03-13 2021-09-16 Pairwise Plants Services, Inc. Methods of introducing variation into plants and products produced therefrom
JP2023525304A (ja) 2020-05-08 2023-06-15 ザ ブロード インスティテュート，インコーポレーテッド 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物
CN119842794A (zh) * 2020-09-10 2025-04-18 孟山都技术公司 利用减数分裂和种系启动子增加基因编辑和定点整合事件
AU2022245134A1 (en) * 2021-03-24 2023-09-14 Syngenta Crop Protection Ag Inducible mosaicism
CN118742647A (zh) 2022-02-15 2024-10-01 富优基尼以色列股份有限公司 对植物保护有用的苏云金芽孢杆菌杀虫蛋白(Bt PP)组合
JP2025510365A (ja) * 2022-03-29 2025-04-14 モンサント テクノロジー エルエルシー 発生プロモーターを使用する遺伝子編集及び部位特異的組込み事象の増加
CN119020353B (zh) * 2023-05-23 2025-05-02 南京农业大学 一种用于不充分编辑靶基因的植物基因编辑方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6828478B2 (en) * 2001-05-09 2004-12-07 The Regents Of The University Of California Combinations of genes for producing seed plants exhibiting modulated reproductive development
US20030208790A1 (en) * 2002-05-03 2003-11-06 Stefan Jansens Insect resistant plants and methods for making same
JP3783777B2 (ja) * 2002-09-20 2006-06-07 独立行政法人農業生物資源研究所 新たな機能を持つジベレリン２−酸化酵素遺伝子およびその利用
WO2006124502A2 (en) * 2005-05-12 2006-11-23 Delta And Pine Land Company An inducible genetic cascade for triggering protein expression in subsequent generations of plants
AU2010217663A1 (en) * 2009-02-25 2011-09-01 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same
WO2014186686A2 (en) * 2013-05-17 2014-11-20 Two Blades Foundation Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases
EP3611268A1 (en) * 2013-08-22 2020-02-19 E. I. du Pont de Nemours and Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
US11186843B2 (en) 2014-02-27 2021-11-30 Monsanto Technology Llc Compositions and methods for site directed genomic modification
EP3383168A4 (en) 2015-12-02 2019-05-08 Ceres, Inc. METHOD FOR THE GENETIC MODIFICATION OF PLANTS

Also Published As

Publication number Publication date
CA3007288A1 (en) 2017-06-08
BR112018011242A2 (pt) 2018-11-21
WO2017096237A1 (en) 2017-06-08
BR112018011242B1 (pt) 2023-11-28
US20200299717A1 (en) 2020-09-24
EP3383168A1 (en) 2018-10-10
EP3383168A4 (en) 2019-05-08
US11802287B2 (en) 2023-10-31
US20210388373A1 (en) 2021-12-16
US11118189B2 (en) 2021-09-14

Similar Documents

Publication Publication Date Title
MX2018006814A (es) Metodos para la modificacion genetica de plantas.
AU2017286122A1 (en) Use of Cpf1 endonuclease for plant genome modifications
AR111790A1 (es) Método para la producción de una planta resistente a herbicidas mediante la introducción de un sistema de edición de bases de un gen relacionado con la resistencia a herbicidas
EP4170029A3 (en) Modifying the specifity of plant non-coding rna molecules for silencing gene expression
MX2025003766A (es) Métodos para identificar, seleccionar y producir cultivos resistentes a la roya sureña del maíz
EA201391373A1 (ru) Способы получения сложного локуса трансгенных признаков
EA201291344A1 (ru) Трансгенное явление mon 88302 у вида brassica и способы его применения
MX2015007574A (es) Gen de precision dirigido a un locus particular en el maiz.
MX2019003063A (es) Promotores específicos de tricoma para la manipulación de cannabinoides y otros compuestos en tricomas glandulares.
AU2018204544B2 (en) Plant regulatory elements and uses thereof
MX391543B (es) Regiones geneticas y genes asociados con un aumento del rendimiento en las plantas.
MX347575B (es) Metodos y medios para modificar un genoma vegetal en una secuencia de nucleotidos usada comunmente en la ingenieria genetica de genomas vegetales.
UA109110C2 (ru) Полинуклеотид, который обеспечивает возможность специфической экспрессии в целых семенах однодольного растения
WO2017180180A3 (en) Plant promoter and 3' utr for transgene expression
MX2019014886A (es) Evento de elite de maiz mzir098.
MX2021014004A (es) Elementos reguladores de plantas y usos de los mismos.
WO2014064705A3 (en) Transgenic plants exhibiting improved resistance to abiotic stress
AU2012260380A8 (en) Plants having enhanced abiotic stress resistance
PH12018501939A1 (en) Plant regulatory elements and uses thereof
MX2018005935A (es) Plantas modificadas que contienen una combinacion de genes de apirasa y metodo para obtener plantas modificadas con combinacion de genes de apirasa.
PH12021550220A1 (en) Plant regulatory elements and uses thereof
PH12022550457A1 (en) Brassica juncea line nubj1207
NZ748198A (en) Plant regulatory elements and uses thereof
RU2015139239A (ru) Транспластомные "косматые" корни
AR105536A1 (es) Métodos y composiciones para el control de hongos-roya mediante la inhibición de la expresión del gen hxt1