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MX2017015517A - Composiciones, sistemas y metodos para secuenciar polinucleotidos usando amarres anclados a polimerasas adyacentes a nanoporos. - Google Patents

Composiciones, sistemas y metodos para secuenciar polinucleotidos usando amarres anclados a polimerasas adyacentes a nanoporos.

Info

Publication number
MX2017015517A
MX2017015517A MX2017015517A MX2017015517A MX2017015517A MX 2017015517 A MX2017015517 A MX 2017015517A MX 2017015517 A MX2017015517 A MX 2017015517A MX 2017015517 A MX2017015517 A MX 2017015517A MX 2017015517 A MX2017015517 A MX 2017015517A
Authority
MX
Mexico
Prior art keywords
polynucleotide
anchored
nanoporos
amarres
polinucleotides
Prior art date
Application number
MX2017015517A
Other languages
English (en)
Other versions
MX393650B (es
Inventor
L Gunderson Kevin
G Mandell Jeffrey
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=56134634&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2017015517(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2017015517A publication Critical patent/MX2017015517A/es
Publication of MX393650B publication Critical patent/MX393650B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/543—Immobilised enzyme(s)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/197—Modifications characterised by incorporating a spacer/coupling moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/607—Detection means characterised by use of a special device being a sensor, e.g. electrode
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Ecology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Una composición incluye un nanoporo que incluye un primero y un segundo lados y una abertura, nucleótidos que incluyen cada uno un marcador alargado y un primer polinucleótido que es complementario a un segundo polinucleótido. Una polimerasa puede disponerse adyacente al primer lado del nanoporo y configurarse para añadir nucleótidos al primer polinucleótido basándose en una secuencia del segundo polinucleótido. Un amarre permanente puede incluir una región de cabeza anclada a la polimerasa, una región de cola y un cuerpo alargado dispuesto entre las mismas que se produce en la abertura del nanoporo. Un primer resto puede estar dispuesto sobre el cuerpo alargado que se une al marcador alargado de un primer nucleótido sobre el que la polimerasa está actuando. Una región indicadora puede estar dispuesta sonre el cuerpo alargado que indica cuándo el primer nucleótido es complementario o no es complementario a un siguiente nucleótido en la secuencia del segundo polinucleótido.
MX2017015517A 2015-06-03 2016-06-02 Composiciones, sistemas y metodos para secuenciar polinucleotidos usando amarres anclados a polimerasas adyacentes a nanoporos MX393650B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562170563P 2015-06-03 2015-06-03
PCT/US2016/035457 WO2016196755A1 (en) 2015-06-03 2016-06-02 Compositions, systems, and methods for sequencing polynucleotides using tethers anchored to polymerases adjacent to nanopores

Publications (2)

Publication Number Publication Date
MX2017015517A true MX2017015517A (es) 2018-11-09
MX393650B MX393650B (es) 2025-03-24

Family

ID=56134634

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2017015517A MX393650B (es) 2015-06-03 2016-06-02 Composiciones, sistemas y metodos para secuenciar polinucleotidos usando amarres anclados a polimerasas adyacentes a nanoporos
MX2022006393A MX2022006393A (es) 2015-06-03 2017-11-30 Composiciones, sistemas y metodos para secuenciar polinucleotidos usando amarres anclados a polimerasas adyacentes a nanoporos.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2022006393A MX2022006393A (es) 2015-06-03 2017-11-30 Composiciones, sistemas y metodos para secuenciar polinucleotidos usando amarres anclados a polimerasas adyacentes a nanoporos.

Country Status (16)

Country Link
US (3) US10648022B2 (es)
EP (2) EP3303627B1 (es)
JP (1) JP6595005B2 (es)
KR (1) KR102828319B1 (es)
CN (2) CN107835858B (es)
AU (1) AU2016270887C1 (es)
CA (1) CA2986074A1 (es)
ES (1) ES3006336T3 (es)
HK (1) HK1250749A1 (es)
IL (3) IL293410B2 (es)
MX (2) MX393650B (es)
MY (1) MY183442A (es)
RU (1) RU2713396C2 (es)
SG (1) SG10202107427PA (es)
WO (1) WO2016196755A1 (es)
ZA (1) ZA201707715B (es)

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JP2018520649A (ja) 2018-08-02
IL293410B2 (en) 2024-01-01
JP6595005B2 (ja) 2019-10-23
ES3006336T3 (en) 2025-03-18
IL293410B1 (en) 2023-09-01
KR20180014159A (ko) 2018-02-07
AU2016270887C1 (en) 2025-01-16
RU2713396C2 (ru) 2020-02-05
MX2022006393A (es) 2022-06-24
IL255758A (en) 2018-01-31
IL304812B1 (en) 2025-12-01
AU2016270887A1 (en) 2017-12-07
HK1250749A1 (zh) 2019-01-11
RU2017139536A3 (es) 2019-07-17
MY183442A (en) 2021-02-18
US20240376535A1 (en) 2024-11-14
CN114703264B (zh) 2024-09-20
US20200318180A1 (en) 2020-10-08
US10648022B2 (en) 2020-05-12
US20180155774A1 (en) 2018-06-07
WO2016196755A1 (en) 2016-12-08
RU2017139536A (ru) 2019-07-09
BR112017025723A2 (pt) 2018-08-14
CN107835858A (zh) 2018-03-23
IL293410A (en) 2022-07-01
CN107835858B (zh) 2022-04-12
KR102828319B1 (ko) 2025-07-02
EP3303627A1 (en) 2018-04-11
EP3303627B1 (en) 2024-12-11
ZA201707715B (en) 2020-01-29
US11970734B2 (en) 2024-04-30
SG10202107427PA (en) 2021-08-30
EP4495255A3 (en) 2025-04-09
CA2986074A1 (en) 2016-12-08
IL255758B (en) 2022-07-01
MX393650B (es) 2025-03-24
AU2016270887B2 (en) 2019-09-19
IL304812A (en) 2023-09-01
EP4495255A2 (en) 2025-01-22
CN114703264A (zh) 2022-07-05

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