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MX2017003790A - Detección de secuencias diana por percepción de nanoporos de sondas sintéticas. - Google Patents

Detección de secuencias diana por percepción de nanoporos de sondas sintéticas.

Info

Publication number
MX2017003790A
MX2017003790A MX2017003790A MX2017003790A MX2017003790A MX 2017003790 A MX2017003790 A MX 2017003790A MX 2017003790 A MX2017003790 A MX 2017003790A MX 2017003790 A MX2017003790 A MX 2017003790A MX 2017003790 A MX2017003790 A MX 2017003790A
Authority
MX
Mexico
Prior art keywords
target sequence
sequence detection
synthetic probes
nanopore sensing
nanopore
Prior art date
Application number
MX2017003790A
Other languages
English (en)
Inventor
A Heller Daniel
J Morin Trevor
Original Assignee
Two Pore Guys Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Two Pore Guys Inc filed Critical Two Pore Guys Inc
Publication of MX2017003790A publication Critical patent/MX2017003790A/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6825Nucleic acid detection involving sensors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/107Modifications characterised by incorporating a peptide nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/164Methylation detection other then bisulfite or methylation sensitive restriction endonucleases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/607Detection means characterised by use of a special device being a sensor, e.g. electrode
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/631Detection means characterised by use of a special device being a biochannel or pore
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/154Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Se describen en la presente metodos y composiciones para la deteccion de unas o mas secuencias especificas de polinucleotidos en una solucion usando un nanoporo. En algunas modalidades, en la presente se describen metodos y composiciones para identificar un polinucleotido en una muestra o para la deteccion de secuencia diana de un polinucleotido.
MX2017003790A 2014-09-26 2015-09-28 Detección de secuencias diana por percepción de nanoporos de sondas sintéticas. MX2017003790A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462056378P 2014-09-26 2014-09-26
PCT/US2015/052729 WO2016049657A1 (en) 2014-09-26 2015-09-28 Target sequence detection by nanopore sensing of synthetic probes

Publications (1)

Publication Number Publication Date
MX2017003790A true MX2017003790A (es) 2017-08-07

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017003790A MX2017003790A (es) 2014-09-26 2015-09-28 Detección de secuencias diana por percepción de nanoporos de sondas sintéticas.

Country Status (11)

Country Link
US (2) US20170349940A1 (es)
EP (1) EP3198036A4 (es)
JP (1) JP6702951B2 (es)
KR (1) KR20170064540A (es)
CN (1) CN107002140A (es)
AU (3) AU2015319825A1 (es)
CA (1) CA2962234A1 (es)
IL (1) IL251274B1 (es)
MX (1) MX2017003790A (es)
RU (1) RU2681822C2 (es)
WO (1) WO2016049657A1 (es)

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AU2022201705A1 (en) 2022-04-07
CA2962234A1 (en) 2016-03-31
RU2017114160A (ru) 2018-10-26
RU2681822C2 (ru) 2019-03-12
IL251274A0 (en) 2017-05-29
EP3198036A4 (en) 2018-08-08
RU2017114160A3 (es) 2018-10-26
US20220195501A1 (en) 2022-06-23
KR20170064540A (ko) 2017-06-09
JP2017529089A (ja) 2017-10-05
WO2016049657A1 (en) 2016-03-31
CN107002140A (zh) 2017-08-01
AU2025203108A1 (en) 2025-05-22
IL251274B1 (en) 2025-09-01
US20170349940A1 (en) 2017-12-07
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JP6702951B2 (ja) 2020-06-03
EP3198036A1 (en) 2017-08-02

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