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MX2016006816A - Promotores sinteticos para celulas de ovario de hamster chino (cho), metodos de produccion de promotores sinteticos usando modulos de sitio de union del factor de transcripcion. - Google Patents

Promotores sinteticos para celulas de ovario de hamster chino (cho), metodos de produccion de promotores sinteticos usando modulos de sitio de union del factor de transcripcion.

Info

Publication number
MX2016006816A
MX2016006816A MX2016006816A MX2016006816A MX2016006816A MX 2016006816 A MX2016006816 A MX 2016006816A MX 2016006816 A MX2016006816 A MX 2016006816A MX 2016006816 A MX2016006816 A MX 2016006816A MX 2016006816 A MX2016006816 A MX 2016006816A
Authority
MX
Mexico
Prior art keywords
synthetic promoters
methods
binding site
transcription factor
cho cells
Prior art date
Application number
MX2016006816A
Other languages
English (en)
Inventor
Cameron JAMES David
John BROWN Adam
Original Assignee
Ucb Biopharma Sprl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB201321109A external-priority patent/GB201321109D0/en
Priority claimed from GB201411717A external-priority patent/GB201411717D0/en
Application filed by Ucb Biopharma Sprl filed Critical Ucb Biopharma Sprl
Publication of MX2016006816A publication Critical patent/MX2016006816A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/24—Vectors characterised by the absence of particular element, e.g. selectable marker, viral origin of replication
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/40—Systems of functionally co-operating vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Ecology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención se relaciona con construcciones del promotor sintético específico de la célula CHO para expresar proteínas recombinantes, una biblioteca de construcciones del promotor de las mismas y un método para producir las construcciones del promotor. Las construcciones del promotor permiten un control preciso de la transcripción del gen recombinante sobre tres órdenes de magnitud, con los promotores se expresión superior capaces de duplicar la actividad transcripcional del promotor de CMV.
MX2016006816A 2013-11-29 2014-11-28 Promotores sinteticos para celulas de ovario de hamster chino (cho), metodos de produccion de promotores sinteticos usando modulos de sitio de union del factor de transcripcion. MX2016006816A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB201321109A GB201321109D0 (en) 2013-11-29 2013-11-29 Synthetic promoters
GB201411717A GB201411717D0 (en) 2014-07-01 2014-07-01 Synthetic promoters
PCT/EP2014/076024 WO2015079053A2 (en) 2013-11-29 2014-11-28 Synthetic promoters

Publications (1)

Publication Number Publication Date
MX2016006816A true MX2016006816A (es) 2016-08-19

Family

ID=52002952

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016006816A MX2016006816A (es) 2013-11-29 2014-11-28 Promotores sinteticos para celulas de ovario de hamster chino (cho), metodos de produccion de promotores sinteticos usando modulos de sitio de union del factor de transcripcion.

Country Status (13)

Country Link
US (1) US20170002378A1 (es)
EP (1) EP3074516A2 (es)
JP (1) JP2016537987A (es)
KR (1) KR20160087903A (es)
CN (1) CN105793415A (es)
AU (1) AU2014356396A1 (es)
BR (1) BR112016012177A2 (es)
CA (1) CA2930344A1 (es)
CL (1) CL2016001195A1 (es)
IL (1) IL245565A0 (es)
MX (1) MX2016006816A (es)
RU (1) RU2016125744A (es)
WO (1) WO2015079053A2 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20240128814A (ko) 2017-01-10 2024-08-27 프레시전 인코포레이티드 신규의 유전자 스위치 발현 시스템을 통한 폴리펩티드의 발현 조절
WO2018169901A1 (en) * 2017-03-13 2018-09-20 Massachusetts Institute Of Technology Synthetic promoters
CN110741086B (zh) * 2017-04-19 2024-04-09 免疫医疗有限公司 具有用户定义的功能的哺乳动物启动子的计算机设计
WO2018213332A1 (en) * 2017-05-17 2018-11-22 Seattle Children's Hospital (dba Seattle Children's Research Institute) Generating mammalian t cell activation inducible synthetic promoters (syn+pro) to improve t cell therapy
CN111902540A (zh) * 2018-03-20 2020-11-06 齐默尔根公司 用于中国仓鼠卵巢细胞基因工程改造的htp平台
CN110343699A (zh) * 2018-04-08 2019-10-18 新乡医学院 用于提高哺乳动物细胞转基因表达的调控序列、表达载体、表达系统及其应用
EP3990647A1 (en) 2019-06-25 2022-05-04 Migal Galilee Research Institute Ltd. Systems and uses thereof for creating synthetic transcriptional logic 'and' gates based on pre-mrna trans-splicing
EP4136237A1 (en) * 2020-04-15 2023-02-22 Asklepios Biopharmaceutical, Inc. Forskolin-inducible promoters and hypoxia-inducible promoters
JP7498598B2 (ja) * 2020-05-29 2024-06-12 花王株式会社 新規プロモーター
JP2024511591A (ja) * 2021-03-16 2024-03-14 アストラゼネカ・アクチエボラーグ 合成プロモーターを使用する多重遺伝子発現の制御
KR102737145B1 (ko) * 2023-07-10 2024-12-09 대한민국 신규 발현 벡터 및 이의 용도

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000034435A2 (en) * 1998-12-08 2000-06-15 Clontech Laboratories, Inc. Cis-element reporter constructs and uses thereof
US20040175727A1 (en) * 2002-11-04 2004-09-09 Advisys, Inc. Synthetic muscle promoters with activities exceeding naturally occurring regulatory sequences in cardiac cells
US7312202B2 (en) * 2003-02-18 2007-12-25 Board Of Regents, The University Of Texas System Rationally designed and chemically synthesized promoter for genetic vaccine and gene therapy
JP4430535B2 (ja) * 2003-07-21 2010-03-10 ロンザ・バイオロジクス・ピーエルシー Ｃｈｏ細胞において組換えタンパク質を発現する方法
JP2008541764A (ja) * 2005-06-01 2008-11-27 バイオテック・インスティチュート・フォー・インターナショナル・イノベーション・インコーポレーテッド グルコース誘導性のインシュリンの発現及び糖尿病を治療する方法
CN101134110B (zh) * 2006-09-01 2012-11-07 中国医学科学院基础医学研究所 Q型非病毒载体以及包含其的药物组合物

Also Published As

Publication number Publication date
BR112016012177A2 (pt) 2017-09-26
IL245565A0 (en) 2016-06-30
JP2016537987A (ja) 2016-12-08
CA2930344A1 (en) 2015-06-04
RU2016125744A (ru) 2018-01-09
CL2016001195A1 (es) 2017-07-28
WO2015079053A2 (en) 2015-06-04
KR20160087903A (ko) 2016-07-22
CN105793415A (zh) 2016-07-20
US20170002378A1 (en) 2017-01-05
AU2014356396A1 (en) 2016-06-02
WO2015079053A3 (en) 2015-10-08
EP3074516A2 (en) 2016-10-05

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