MX2016001396A - Seleccion novedosa de vectores y metodos de seleccion de celulas hospederas eucarioticas. - Google Patents
Seleccion novedosa de vectores y metodos de seleccion de celulas hospederas eucarioticas.Info
- Publication number
- MX2016001396A MX2016001396A MX2016001396A MX2016001396A MX2016001396A MX 2016001396 A MX2016001396 A MX 2016001396A MX 2016001396 A MX2016001396 A MX 2016001396A MX 2016001396 A MX2016001396 A MX 2016001396A MX 2016001396 A MX2016001396 A MX 2016001396A
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- host cells
- eukaryotic host
- selection vectors
- folate receptor
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 239000013598 vector Substances 0.000 title 1
- 239000013604 expression vector Substances 0.000 abstract 4
- 102000006815 folate receptor Human genes 0.000 abstract 3
- 108020005243 folate receptor Proteins 0.000 abstract 3
- 229920001184 polypeptide Polymers 0.000 abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 229940014144 folate Drugs 0.000 abstract 1
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 abstract 1
- 235000019152 folic acid Nutrition 0.000 abstract 1
- 239000011724 folic acid Substances 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 238000010187 selection method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/40—Systems of functionally co-operating vectors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
La invención se refiere a un vector de expresión o una combinación de al menos dos vectores de expresión que comprenden: a) un polinucleótido que codifica un receptor de folato mutado como marcador seleccionable, en donde el receptor de folato mutado tiene una disminución de la afinidad de unión de folato en comparación con el receptor de folato tipo silvestre; b) al menos un polinucleótido que codifica un polipéptido de interés, en donde cuando dicho vector de expresión o combinación de al menos dos vectores de expresión se introduce en una célula hospedera, el polipéptido de interés se secreta a partir de dicha célula hospedera. También se proporcionan células hospederas adecuadas, métodos de selección y métodos para la producción de polipéptidos con alto rendimiento.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361860439P | 2013-07-31 | 2013-07-31 | |
| PCT/IB2014/063517 WO2015015419A1 (en) | 2013-07-31 | 2014-07-29 | Novel selection vectors and methods of selecting eukaryotic host cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2016001396A true MX2016001396A (es) | 2016-05-05 |
| MX369109B MX369109B (es) | 2019-10-29 |
Family
ID=51383909
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016001396A MX369109B (es) | 2013-07-31 | 2014-07-29 | Selección novedosa de vectores y métodos de selección de células hospederas eucarióticas. |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US11807855B2 (es) |
| EP (3) | EP4095153A1 (es) |
| JP (1) | JP6576924B2 (es) |
| KR (1) | KR102288233B1 (es) |
| CN (2) | CN105593239B (es) |
| AU (1) | AU2014297966B2 (es) |
| BR (1) | BR112016001182A2 (es) |
| CA (1) | CA2918117C (es) |
| DK (2) | DK3412684T3 (es) |
| ES (2) | ES2920510T3 (es) |
| HU (1) | HUE058885T2 (es) |
| IL (1) | IL243352B (es) |
| LT (2) | LT3027646T (es) |
| MX (1) | MX369109B (es) |
| PL (2) | PL3027646T3 (es) |
| PT (2) | PT3027646T (es) |
| RU (1) | RU2716977C2 (es) |
| SG (1) | SG11201510625TA (es) |
| WO (1) | WO2015015419A1 (es) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101707251B1 (ko) | 2009-02-27 | 2017-02-15 | 노파르티스 아게 | 2종의 선택 마커를 포함하는 발현 벡터 시스템 |
| KR102288233B1 (ko) | 2013-07-31 | 2021-08-10 | 노파르티스 아게 | 신규한 선택 벡터 및 진핵 숙주 세포의 선택 방법 |
| HUE069884T2 (hu) * | 2015-10-09 | 2025-04-28 | Genzyme Corp | Javított flare (áramlási citometriával csillapított reporter expresszió) technológia gyors tömeges válogatáshoz |
| EP3521435A1 (en) | 2018-02-06 | 2019-08-07 | Bayer AG | A high stringency selection system for protein expression in proline-auxotrophic cells |
| CN112673109A (zh) * | 2018-09-07 | 2021-04-16 | 免疫医疗有限责任公司 | 细胞选择方法 |
| US20220348632A1 (en) | 2019-09-18 | 2022-11-03 | Novartis Ag | Nkg2d fusion proteins and uses thereof |
| CN116615462A (zh) | 2020-11-23 | 2023-08-18 | 诺华股份有限公司 | 抗体构建体的表达技术 |
| WO2022229854A1 (en) | 2021-04-29 | 2022-11-03 | Novartis Ag | Hypersialylating cells |
| US20250051758A1 (en) * | 2021-12-21 | 2025-02-13 | Absci Corporation | FolA Selection Assay to Identify Strains with Increased Soluble Target Protein Expression |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4771036A (en) | 1986-02-10 | 1988-09-13 | Trustees Of Columbia University In The City Of New York | Method and ophthalmic composition for the prevention and reversal of cataracts |
| JP2585532B2 (ja) | 1986-05-10 | 1997-02-26 | 三井東圧化学株式会社 | 動物細胞の形質転換体及びそれを得る方法並びにその形質転換体を用いてｔ−ＰＡを生産する方法 |
| JP3150340B2 (ja) | 1990-11-13 | 2001-03-26 | イムネクス コーポレイション | 二機能選択可能融合遺伝子 |
| EP0724639B1 (en) | 1991-03-29 | 2001-01-31 | Genentech, Inc. | Methods for selection of recombinant host cells expressing high levels of a desired protein |
| JPH09500783A (ja) | 1993-05-21 | 1997-01-28 | ターゲッティッド ジェネティクス コーポレイション | シトシンデアミナーゼ（ｃｄ）遺伝子に基づく二機能性選択融合遺伝子 |
| US5439808A (en) | 1993-07-23 | 1995-08-08 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis |
| US5830698A (en) | 1997-03-14 | 1998-11-03 | Idec Pharmaceuticals Corporation | Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same |
| US6106825A (en) | 1997-05-07 | 2000-08-22 | University Of Florida | Entomopoxvirus-vertebrate gene delivery vector and method |
| FR2784030B1 (fr) | 1998-10-02 | 2002-12-20 | Inst Nat Sante Rech Med | Utilisation de bloqueurs des canaux calciques et/ou cgmp-dependants pour le traitement de pathologies de la retine |
| FR2823221B1 (fr) | 2001-04-06 | 2004-04-02 | Univ Pasteur | Sequences associees a la degenerescence retinienne et applications |
| DE10256083A1 (de) | 2002-11-29 | 2004-08-12 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Expressionsvektor, Verfahren zur Herstellung von heterologen Genprodukten und Selektionsverfahren für hochproduzierende rekombinante Zellen |
| US7384744B2 (en) | 2002-11-29 | 2008-06-10 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products |
| MXPA05009608A (es) * | 2003-03-11 | 2006-03-21 | Applied Research Systems | Vectores de expresion que comprenden el promotor mcmv ie2. |
| DE602005016218D1 (de) | 2004-01-30 | 2009-10-08 | Maxygen Aps | Gesteuertes überlesen von stopcodons |
| US20060275794A1 (en) | 2005-03-07 | 2006-12-07 | Invitrogen Corporation | Collections of matched biological reagents and methods for identifying matched reagents |
| KR101328300B1 (ko) | 2006-02-21 | 2013-11-14 | 크로마제닉스 비.브이. | 고수준으로 단백질을 발현하는 숙주세포의 선택 |
| JP4870208B2 (ja) | 2006-05-17 | 2012-02-08 | エフ．ホフマン−ラ ロシュ アーゲー | ポリペプチド産生細胞 |
| PT2227549E (pt) | 2007-12-21 | 2015-10-08 | Novartis Ag | Sistema de seleção para cultura de células eucarióticas com base num gene de recetor de folato ligado à membrana |
| EP2227546B1 (en) | 2007-12-21 | 2016-05-04 | Novartis AG | Mammalian expression vector |
| RU2528858C2 (ru) | 2008-08-28 | 2014-09-20 | Новартис Аг | Дисплей на поверхности клеток полипептидных изоформ на основе прочитывания терминирующего кодона |
| JP5748223B2 (ja) | 2008-09-10 | 2015-07-15 | アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル | ニューロン生存因子およびその使用 |
| MX2011009023A (es) | 2009-02-27 | 2011-09-28 | Novartis Ag | Metodos para seleccionar celulas eucarioticas que expresan una proteina heterologa. |
| KR101707251B1 (ko) | 2009-02-27 | 2017-02-15 | 노파르티스 아게 | 2종의 선택 마커를 포함하는 발현 벡터 시스템 |
| CN102030754B (zh) * | 2009-09-28 | 2013-07-10 | 中国科学院微生物研究所 | 非病毒载体及其制备方法与应用 |
| KR102288233B1 (ko) | 2013-07-31 | 2021-08-10 | 노파르티스 아게 | 신규한 선택 벡터 및 진핵 숙주 세포의 선택 방법 |
-
2014
- 2014-07-29 KR KR1020167002307A patent/KR102288233B1/ko active Active
- 2014-07-29 LT LTEP14753158.6T patent/LT3027646T/lt unknown
- 2014-07-29 PT PT14753158T patent/PT3027646T/pt unknown
- 2014-07-29 US US14/907,866 patent/US11807855B2/en active Active
- 2014-07-29 ES ES18179678T patent/ES2920510T3/es active Active
- 2014-07-29 CN CN201480054263.9A patent/CN105593239B/zh active Active
- 2014-07-29 DK DK18179678.0T patent/DK3412684T3/da active
- 2014-07-29 PL PL14753158T patent/PL3027646T3/pl unknown
- 2014-07-29 PT PT181796780T patent/PT3412684T/pt unknown
- 2014-07-29 JP JP2016530647A patent/JP6576924B2/ja active Active
- 2014-07-29 ES ES14753158.6T patent/ES2689136T3/es active Active
- 2014-07-29 EP EP22166577.1A patent/EP4095153A1/en not_active Withdrawn
- 2014-07-29 MX MX2016001396A patent/MX369109B/es active IP Right Grant
- 2014-07-29 CA CA2918117A patent/CA2918117C/en active Active
- 2014-07-29 PL PL18179678.0T patent/PL3412684T3/pl unknown
- 2014-07-29 HU HUE18179678A patent/HUE058885T2/hu unknown
- 2014-07-29 LT LTEP18179678.0T patent/LT3412684T/lt unknown
- 2014-07-29 WO PCT/IB2014/063517 patent/WO2015015419A1/en not_active Ceased
- 2014-07-29 EP EP18179678.0A patent/EP3412684B1/en active Active
- 2014-07-29 EP EP14753158.6A patent/EP3027646B1/en active Active
- 2014-07-29 RU RU2016106270A patent/RU2716977C2/ru active
- 2014-07-29 SG SG11201510625TA patent/SG11201510625TA/en unknown
- 2014-07-29 CN CN202010947233.8A patent/CN112029791A/zh active Pending
- 2014-07-29 DK DK14753158.6T patent/DK3027646T3/en active
- 2014-07-29 BR BR112016001182A patent/BR112016001182A2/pt not_active Application Discontinuation
- 2014-07-29 AU AU2014297966A patent/AU2014297966B2/en active Active
-
2015
- 2015-12-24 IL IL243352A patent/IL243352B/en active IP Right Grant
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |