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MX2012008148A - Conjugados de igf-i poli(etilenglicol). - Google Patents

Conjugados de igf-i poli(etilenglicol).

Info

Publication number
MX2012008148A
MX2012008148A MX2012008148A MX2012008148A MX2012008148A MX 2012008148 A MX2012008148 A MX 2012008148A MX 2012008148 A MX2012008148 A MX 2012008148A MX 2012008148 A MX2012008148 A MX 2012008148A MX 2012008148 A MX2012008148 A MX 2012008148A
Authority
MX
Mexico
Prior art keywords
poly
ethylene glycol
nucleic acid
expression construct
acid encoding
Prior art date
Application number
MX2012008148A
Other languages
English (en)
Other versions
MX339559B (es
Inventor
Friederike Hesse
Eva Hoess
Stephanie Mueller
Eva Maria Trost-Gross
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2012008148A publication Critical patent/MX2012008148A/es
Publication of MX339559B publication Critical patent/MX339559B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
    • A61K47/60—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1075—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of amino acids or peptide residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1019—Tetrapeptides with the first amino acid being basic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Epidemiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Biomedical Technology (AREA)
  • Hospice & Palliative Care (AREA)
  • Psychiatry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un método para producir un polipéptido conjugado a un poli(etilenglicol) que comprende a) proporcionar un ácido nucleico que codifica un constructo de expresión que comprende en la dirección 5' a 3' un ácido nucleico que codifica un polipéptido, y un ácido nucleico que codifica un sitio de tripsina de SEC ID NO: 01, b) expresar el ácido nucleico de a) en una célula y recuperar el constructo de expresión de la célula y/o el medio de cultivo, c) proporcionar un péptido objetivo con una secuencia de aminoácido de SEC ID NO: 02 covalentemente conjugado a un poli(etilenglicol) en el residuo lisina C-terminal, d) incubar el constructo de expresión y el péptido de objetivo con la tripsina mutante D189K, 1C60E, N143H, E151H, y e) recuperar y producir el polipéptido conjugado a un poli(etilenglicol) de la mezcla de incubación.
MX2012008148A 2010-02-11 2011-02-04 Conjugados de igf-i poli(etilenglicol). MX339559B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10153275 2010-02-11
PCT/EP2011/051631 WO2011098400A1 (en) 2010-02-11 2011-02-04 Igf-i poly (ethylene glycol) conjugates

Publications (2)

Publication Number Publication Date
MX2012008148A true MX2012008148A (es) 2012-08-03
MX339559B MX339559B (es) 2016-05-31

Family

ID=42199427

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012008148A MX339559B (es) 2010-02-11 2011-02-04 Conjugados de igf-i poli(etilenglicol).

Country Status (11)

Country Link
US (2) US9587006B2 (es)
EP (1) EP2533813B1 (es)
JP (2) JP5749744B2 (es)
KR (1) KR101485646B1 (es)
CN (1) CN102740896B (es)
BR (1) BR112012019992A2 (es)
CA (1) CA2786340C (es)
ES (1) ES2587574T3 (es)
MX (1) MX339559B (es)
RU (1) RU2584572C2 (es)
WO (1) WO2011098400A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180059922A (ko) * 2015-10-02 2018-06-05 유니버시티 오브 코펜하겐 히스톤 리더 도메인을 차단하는 소분자
US10738338B2 (en) 2016-10-18 2020-08-11 The Research Foundation for the State University Method and composition for biocatalytic protein-oligonucleotide conjugation and protein-oligonucleotide conjugate
EP3650541A1 (en) * 2018-11-08 2020-05-13 BioPharma Translationsinstitut Dessau Forschungs GmbH Trypsin mutant for c- and n-terminal modification of polypeptides
CN112546228A (zh) * 2019-09-10 2021-03-26 中国科学院上海营养与健康研究所 非igf1r结合型的物质在预防和/或治疗炎症性疾病中的应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3051145B2 (ja) 1990-08-28 2000-06-12 住友製薬株式会社 新規なポリエチレングリコール誘導体修飾ペプチド
ATE119916T1 (de) * 1990-12-10 1995-04-15 Hoffmann La Roche Verfahren zur enzymatischen herstellung von grf(1-44)nh2.
ATE225801T1 (de) 1992-07-13 2002-10-15 Bionebraska Inc Verfahren zur modifizierung rekombinanter polypeptide
WO1994012219A2 (en) 1992-11-25 1994-06-09 Amgen Boulder Inc. Modified insulin-like growth factors
EP0756494A1 (en) * 1994-05-24 1997-02-05 Amgen Boulder Inc. Modified insulin-like growth factors
US5932462A (en) 1995-01-10 1999-08-03 Shearwater Polymers, Inc. Multiarmed, monofunctional, polymer for coupling to molecules and surfaces
CZ300546B6 (cs) 1999-01-29 2009-06-10 Amgen, Inc. Fyziologicky aktivní konjugát, prostredek a zpusob prípravy
US6303337B1 (en) * 1999-08-25 2001-10-16 Amber Gen. Inc. N-terminal and C-terminal markers in nascent proteins
DE10049673A1 (de) 2000-10-07 2002-04-25 Univ Leipzig Verfahren zur Herstellung von Peptiden, Peptidmimetika und Proteinen
DE10205091B4 (de) * 2002-02-07 2007-04-19 Biomax Informatics Ag Verfahren zur Vorhersage der Expressionseffizienz in zellfreien Expressionssystemen
EP1778839B1 (en) * 2004-08-13 2008-07-09 Roche Diagniostics GMBH C-terminal modification of polypeptides
EP1674113A1 (en) * 2004-12-22 2006-06-28 F. Hoffmann-La Roche Ag Conjugates of insulin-like growth factor-1 (IGF-1) and poly(ethylene glycol)
EP2045265B1 (en) * 2005-09-22 2012-11-21 Biocompatibles Uk Ltd. GLP-1 (Glucagon-like peptide-1) fusion polypeptides with increased peptidase resistance
DE102005046113A1 (de) 2005-09-27 2007-03-29 Sanofi-Aventis Deutschland Gmbh Verfahren zur Amidierung von Polypetiden mit C-terminalen basischen Aminosäuren unter Verwendung spezifischer Endoproteasen
CL2007002502A1 (es) * 2006-08-31 2008-05-30 Hoffmann La Roche Variantes del factor de crecimiento similar a insulina-1 humano (igf-1) pegilados en lisina; metodo de produccion; proteina de fusion que la comprende; y su uso para tratar la enfermedad de alzheimer.
JP2009207449A (ja) * 2008-03-06 2009-09-17 Univ Nagoya ラミニンコイルドコイル（ｌｃｃ）ドメインヘテロ多量体を含むタンパク質会合体の調製方法

Also Published As

Publication number Publication date
EP2533813B1 (en) 2016-06-01
KR20120137364A (ko) 2012-12-20
WO2011098400A1 (en) 2011-08-18
CA2786340C (en) 2017-08-22
JP6014194B2 (ja) 2016-10-25
HK1171375A1 (en) 2013-03-28
CN102740896B (zh) 2014-08-27
CN102740896A (zh) 2012-10-17
ES2587574T3 (es) 2016-10-25
MX339559B (es) 2016-05-31
JP5749744B2 (ja) 2015-07-15
US9587006B2 (en) 2017-03-07
US20120309679A1 (en) 2012-12-06
RU2012138553A (ru) 2014-03-20
CA2786340A1 (en) 2011-08-18
BR112012019992A2 (pt) 2020-08-18
JP2015145402A (ja) 2015-08-13
RU2584572C2 (ru) 2016-05-20
US20170267736A1 (en) 2017-09-21
JP2013518922A (ja) 2013-05-23
EP2533813A1 (en) 2012-12-19
KR101485646B1 (ko) 2015-01-22

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