MX2011009769A - Bactericide composition of organic origin for inducing resistance for preventing, controlling and/or removing phytopathogen diseases from agricultural crops. - Google Patents
Bactericide composition of organic origin for inducing resistance for preventing, controlling and/or removing phytopathogen diseases from agricultural crops.Info
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Abstract
The present invention is a bactericide composition of organic origin for inducing resistance useful in agricultural crops in general, which is essentially formed by one or more: (a) vegetable extracts, (b) vegetable oils, (c) animal origin derivatives, (d) microorganisms and (e) conditioners and/or diluents. The present invention is a complex of organic molecules, which represents an alternative with a high bactericide and bacteriostatic action at a cell level, avoiding the formation of membranes and blocking the oxidative phosphorylation for obtaining energy, thus having a wide control spectrum, acting directly over gram positive and gram negative bacteria, inhibiting the development thereof and removing the largest formation of the same. The invention is also a powerful antiseptic, which causes cell lysis in bacteria, removing the selection of resistant bacteria strains. The present invention is therefore effective against all type of bacteria, especially those of Acidovorax, Clavi bacter, Erwinia, Pseudomonas, Erwinia, Ralstonia, Xanthomonas, Agrobacterium, Streptomyces, Actinobacteria, Curtobacterium, Micoplasma, Spiroplasma and Fitoplasma genera, also inducing systemic resistance at physiological level for strengthening organs susceptible to be damaged, ensuring an effective control and/or reducing in a significant manner the possibility of bacteria evolution, due to the integral action of the composition.
Description
COMPOSITION BACTERICIDE AND INDUCTOR OF RESISTANCE OF ORGANIC ORIGIN FOR THE PREVENTION, CONTROL AND / OR ELIMINATION OF
FITOPATHOGENIC DISEASES IN AGRICULTURAL CROPS
DESCRIPTION
OBJECT OF THE INVENTION
The present invention relates to a new bactericidal composition and inducer of
resistance of organic origin, for the prevention, control and / or elimination of diseases
caused by phytopathogenic bacteria in agricultural crops, based on extracts and oils
vegetables, derivatives of animal origin, microorganisms, emulsifiers and acidifiers
organic and conditioners and / or organic diluents.
BACKGROUND
Due to the continuous growth of the human population, it has been necessary to define new
Strategies to achieve a constant production of food that meets the needs
of humanity. One of the mechanisms currently in process to meet said
need is focused on the reduction of losses in agricultural crops that are caused
by pests and microorganisms that produce diseases (Altieri and Nicholls, 2000).
The most frequent causes in the low agricultural production are the poor growth of
the plants and the destruction of crops by biotic and abiotic factors. Among the causes of biological origin are weeds, insect pests and phytopathogean microorganisms. Each year, plant diseases caused by pathogens such as fungi, bacteria, nematodes or viruses decrease production in crops in all areas of the world, so that the economic losses derived from biological aggression to agricultural crops are considerable and their Prevention and eradication are a priority task.
Worldwide, agricultural crops have been seriously affected by the excessive increase of insect pests, viruses, bacteria and fungi, which cause devastating diseases such as late blight of potato and tomato caused by Phytophthorainfestans and Phytophthoracapsici and Fusarium in Chile, as well as bacteria of the genera Pseudomonas, Xanthomas and Clavibacter, that if proper management is not carried out, losses can reach 100% of production and, together with insects, make it even more difficult to produce agricultural crops.
In the case of bacteria, they spread through cultural practices, through cuttings of contaminated plants, or through the soil itself, through the use of contaminated substrates. They can also be transmitted by insects and nematodes. They always penetrate by stomata or by an existing lesion, because they lack differentiated organs that allow them to penetrate mechanically in the tissues of the plant. To fight against bacteria, prophylactic measures are necessary above all, being able to resort to the chemical fight, use of healthy material, biological fight, etc. Bacteria typically colonize intercellular spaces in different organs or the xylem surrounded by superficial polysaccharides, mainly exopolysaccharides that increase their virulence. Many secrete toxins that cause cellular damage, including enzymes that degrade cell walls. The symptoms of the diseases they produce are not always typical and can be confused with those caused by diseases due to fungi or other agents. The damages that they produce can be tumors, wilts, dry necrosis or soft rottenness.
Among the main genera of phytopathogenic bacteria of agricultural importance, they areErv w'a, Pseudomona, RalstoniaXanthomona, Clavibacter, Acidovorax, Agrobacterium, CurtobacteriumStreptomyces, Actinobacteria, Spiroplasmas andFitoplasmas.
Erwinia is a genus of bacteria of the family Enterobacteriaceae, all species of this genus are pathogenic for plants, highlighting Erwiniaamylovora, which causes necrotic diseases of fruit trees (Hernández and Trujillo, 2004) "called firebomb (Ordaxet al, 2009 ); and Erwiniacarotovora causing the wet and / or soft rot of potatoes and carrots: Pectobacterium (Hemández and Trujillo, 2004).
Among the bacteria of the genus Pseudomona, the most important agricultural species are P. syringae, which is a prolific plant pathogen (Young, 1970). It exists in more than 50 variants, many of which show a high degree of specificity in plants, so there are numerous species of other Pseudomonas that can act as pathogens in plants, although the P.syringae is the most distributed and best studied , although it is not a strictly plant pathogen; however, P. tolaasii can be a major agricultural problem, because it causes bacterial spots of cultured mushrooms (Brodey, 1991). Similarly, P. agarici can cause gout flakes in certain mushrooms (Young, 1970). And the species of P. fuscoveginae, is responsible for the bacteriosis in rice, easily dispersed by the mechanical workings of the soil, the water of irrigation and mainly by the seed of infected rice; climatic conditions of high humidity favor the development of the disease. Other strains previously classified in the genus Pseudomonas are now grouped in the genera Burkholderia and Ralstonia (wdey, 1991).
Of the genus Ralstonia, one of the most studied species in the agricultural sector corresponds to Ralstoniasolanacearum, which is a soil bacterium that colonizes the phloem of plants, causing bacterial wilt in a wide range of host plants, thus causing the disease of bacterial wilt or brown rot, which is one of the most serious diseases in tubers, mainly enpapa, in warm regions of the world, restricting the production of this crop (International Potato Center, 1999); It is worth mentioning that it is also the causative agent of Moko disease, constituting one of the most important banana and plantain problems in the world, since the losses caused are huge, due to the fact that the infected plants must be eradicated. Surveillance and control must be permanent (Surveillance and Pest Control Management of Costa Rica, 2010).
The genus Xanthomonas, is the agent responsible for bacterial spots, as well as the formation of scabs on vegetables (Aranda, 2002). Within this genus stands out the species AcidovoraxavenaeS bsp. CitruUi, which is responsible for the bacterial spot in fruits, specifically in melon and watermelon.
The bacteria of the genus Clavibacter, are responsible for the disease of the chancre or bacterial cancer, systemic disease that produces wilting and death of plants; It is the main bacteriosis worldwide in agricultural crops of vegetables, and is considered as a quarantine organism in the EU (de León et al, 2009).
As for the genus Acidovorax, this is responsible for the bacterial or aqueous spot in fruits (de León et al, 2009).
Agrobacterium is a genus of bacteria that cause tumors in plants. Rhizobiumradiobacter or Agrobacteriumtumefaciens, is the most commonly studied species of this group, in plants it causes the disease of the neck gills, so named because it produces galls or tumors, often in the area where the root is attached to the stem (neck, or called corona), which are produced by the transfer of a DNA segment (T-DNA) of the bacterial plasmid Ti (abbreviation of tumor-inducer) (Francis and Spiker, 2005). The closely related species, R. rhizogenes (= A. rhizogenes), induces tumors in the root, and presents a distinct plasmid Ri (short for root-inducer). The species R. vitis (= A. vitis), generally restricted to the vine, may have a Ti plasmid. The plasmid DNA-T is integrated semi-randomly in
the genome of the host cell carrying genes of biosynthetic enzymes for the production of unusual amino acids, typically octopine or nopaline, also carries genes for the biosynthesis of plant hormones, auxins and cytokinins; and T-DNA virulence genes are expressed, so that the plant can not control cell division, causing the formation of a gill or tumor, it is worth mentioning that the ratio of auxin to cytokinin determines the morphology of the tumor (Francis and Spiker, 2005).
Of the bacterial genus Curtobacterium, the species Curtobacterium flaccumfaciens or Corynebacterium flaccumfaciens stands out, it is considered of quarantine importance, there is no information on the variation of the breed and it is responsible for the bacterial spot in beans and soybeans (EPPO / CABI, 1996). In the field, it survives in the soil for at least two winters between the bean crops in rotation with the wheat, for the moment, there are no reports of the vectors, but the Meloidogyneincognita nematode, can help the entrance, providing the wounds and Once inside the plant, it colonizes the vascular tissue, (EPPO / CABI, 1996). The disease is characterized by a withering of the leaves or parts of the plants during the heat of the day, as a result of connecting the bacterial vessels in the plant, the water supply is cut off and the leaves turn brown; occasionally, these typical wilt symptoms may be absent and replaced by golden yellow, necrotic lesions of leaves, very similar to those of common blight caused by the genus Xanthomona (EPO / CABI, 1996).
The Actinobacteria or Actinomycetes are found on Earth, and include some of the most typical forms of terrestrial life, playing an important role in the decomposition of organic matter, such as cellulose and chitin, renewing the nutrient reserves in the earth and are fundamental in the formation of humus.
The genus Streptomyces is the most extensive of Actinobacteria, are a group of bacteriagram positive and are found predominantly in soils and in decomposed vegetation, most of which produces spores (also called conidia) at the ends of the aerial hyphae (Madigan and Martinico, 2005 ). They are distinguished by the smell of moist earth that they give off, as a result of the production of a volatile metabolite, geosmin. In plants, the most important species are S. caviscabies and S. scabies, which cause scabs (Madigan and Martinico, 2005).
Spiroplasma is a genus of Mollicutes, where most species of this genus are found either in the gut or hemolymph of insects or in the phloem of plants; the best-studied species are Spiroplasmacitri, the agent responsible for orange tree disease (Stuborn), and Spiroplasmakunkelii, a mast disease mast (Stunt) (Jiggins, 2000).
Another microorganism of agricultural importance is Phytoplasma in Latin: Phytoplasma, which is a parasite of plants, apparently of the Mollicutes class, in which their survival is possible only in the interior of host plants, are considered intermediate forms between viral entities and bacteria; the difference of the bacteria are mainly in the cell wall and this leads them to assume very varied forms; possessing, however, a membrane of a protein-glucidic and lipid nature. Do not
They have a true nucleus even if they contain more DNA than RNA. They develop causing diseases in the plants, locating in the phloem, invading the viafloematic of the plant giving rise to the typical symptoms of foliar wrinkling; in some cases, necrosis is formed; A typical example of phytoplasma is the causative agent of lethal yellowing called golden flavescence of the vine, transmitted by the emipteioScaphoideustitanus being transmitted in the wild by Hemiptera insects (First International Phytoplasmologist, 2007).
There are several methods to achieve control of diseases in plants, caused by bacteria, one of them is the use of formulations and / or agricultural chemicals, which are now widely used worldwide as bactericides, with the intention of improving the production of agricultural crops. However, the excessive and indiscriminate application of bactericides of chemical origin, for the control of agricultural diseases, has caused a number of problems of resistance, environmental damage and serious complications to human health. In addition to raising production costs by the use of this class of products.
On the other hand, the demand for agricultural products with less ecological impact, is increasing, in order to reduce environmental pollution, as well as obtaining safe products, highlighting the importance of seeking new technologies for the control of pests and diseases of plants .
Within the scope of the control of diseases in agricultural crops, caused by phytopathogenic bacteria, through the use of products of natural origin, which are compatible with the environment, European patent WO2006CN01789, which describes dequitosano bases as agricultural bactericides and their use, stands out. which have a high activity and very good compatibility with the environment. On the other hand, the present invention consists of a bactericidal and resistance-inducing composition of organic origin, to prevent, control and / or eliminate diseases caused by bacteria in vegetable crops, from extracts and oils of plants, among other components, combined to the addition of bacteria of the genus Bacillus.
As well as US Pat. No. 7,973,006, which consists of an antibacterial composition against gram-negative bacteria, comprising the combination of esters of fatty acids and hydroxycarboxylic acids with an antibacterial agent selected from polylysine, protamine, its salts and mixtures thereof. However, the present invention consists of a bactericidal and resistance-inducing composition of organic origin, to prevent, control and / or eliminate diseases caused by gram-positive and gram-negative bacteria in agricultural crops, from extracts and vegetable oils, in addition to the addition of Bacillus bacteria, among other components.
Also one of the methods that are being used to protect plants against diseases caused by phytopathogenic microorganisms, is the use of compositions and / or systemic treatments that help to inhibit and induce systemic resistance in plants against said microorganisms, based on salts and / or resistance-inducing microorganisms, extracts and vegetable oils.
Such is the case of the United States of America patent US 2004/024242 Al, which refers to a composition for the protection induced by plants against nematodes, which comprises acids and salts, hydrolyzed microorganisms (Bacillussp.), Extract Aqueous of calendula and salicylic acid. On the other hand, the present invention deals with a bactericidal and resistance-inducing composition as an organic treatment, for preventing, controlling and / or eliminating diseases caused by bacteria of the genus Erwinia, Pseudomonas, RalstoniaXanthomona, Clavibacter, Acidovorax,
Agrobacter ium, CurtobacteriumStreptomyces, Actinobacteria, Spiroplasmas andFitoplasmas in agricultural crops, through the use of plant extracts (Alliumsativumüesco Garlic, Equinacia angustifolia, C toronja seed / trus paradisiaca, Larrea tridentata, pollen-propóleo, Jamaica flowerHibiscussabdariffa, marine algae, tepezcohuite / mosa tenuiflora , guiche de lechuguilla ígízve lechuguilla), vegetable oils (Oregano Lippiagraveolens), derivatives of animal origin (Chitosan hydrolyzate and macdx oil aDecapterusmuroadsi), bacteria of the genus Bacillus and natural additives.
As well as of the United States of America patent US 2004/0242419 Al, referring to a systemic plant-inducing composition, from acids and salts, microorganisms (Bacillus), growth inducers (Betaine, cytokinins, auxins, etc.) and plant extracts (neem oil and / or calendula). The present invention consists of a bactericidal and resistance-inducing composition as an organic treatment, for
prevent, control and / or eliminate diseases caused by bacteria of the genus Erwinia, Pseudomonas, RalstoniaXanthomona, Clavibacter, Acidovorax,
Agrobacterium, CurtobacteriumStreptomyces, Actinobacteria, Spiroplasmas andFitoplasms in agricultural crops, through the use of plant extracts (Alliumsativumíresco garlic, Equinacia angustifolia, grapefruit seed Citrus paradisiaca, Larrea tridentata, pollen-propóleo, Jamaica flowerHibiscussabdariffa, marine algae, tepezcohuite / mosa tenuiflora, guiche de lechuguill 4gave lechuguilla), vegetable oils (Oregano Lippiagraveolens ), deúvados of animal origin (Chitosan hydrolyzed and vaacartXaDecapterusmuroadsi oil), bacteria of the Bacillus genus and natural additives.
U.S. Patent US 6,582,961 Bl, about a method for increasing the induction of systemic resistance in crops of edible plants for humans, against phytopathogenic microorganisms (insects, fungi, bacteria, viruses and nematodes) by using inducers of resistance (humic acids, peracetic acid, marine algae, salicylic acid and jasmonic acid), reactive oxygen species and inducing microorganisms of the genus Bacillus, Serratia, Pseudomona and Tricoderma. In contrast, the present novelty consists of an antibacterial composition of organic origin, to prevent, control and / or eliminate diseases caused by phytopathogenic bacteria in agricultural crops from plant extracts (Alliumsativumfresco garlic, Equinacia angustifolia, C toronja seed rrws paradisiaca, Larrea tridentata , pollen-propóleo, flower of JamaicaHibiscussabdariffa, seaweed, tepezcohuite zmosa tenuiflora, guiche de lechuguilla ígave lechuguilla), vegetable oils (OréganoLippiagraveolens), derivatives of animal origin (Chitosan hydrolyzate and oil of m &coxtXdJDecapterusmuroadsi) and bacteria of the genus Bacillus.
U.S. Patent US 2004/0242419 Al, which indicates a systemic inducer treatment of the growth and protection of plants against insects, fungi, bacteria and viruses; mainly Xanthomonas, Rhodotor lum, Tricoderma, Pseudomona and species 937b and SE 34 of Rhizobacterium, through the use of salicylic acid, oxalic acid and / or salts of phosphorus, potassium, calcium, sodium, magnesium, manganese, zinc, iron and copper, microorganism of the genus Bacillus hydrolyzed or live, inducers of growth and vegetable extracts of calendula, tree tea, garlic and vanilla and Neem oil. The present invention deals with a bactericidal and resistance-inducing composition of organic origin, for preventing, controlling and / or eliminating diseases caused by bacteria of the genus Erwinia, Pseudomonas, RalstoniaXanthomona, Clavibacter, Acidovorax,
Agrobacterium, CurtobacteriumStreptomyces, Actinobacteria, Spiroplasmas andFitoplasmas in agricultural crops, through the use of plant extracts (Alliumsativumfrcsco Garlic, Equinacia angustifolia, Paradise grapefruit C / írwi ', Larrea tridentata, pollen-propóleo, Jam & ibed ibiscussabdariffa flower, marine algae, tepezcohuite / wosa tenuiflora, guiche de lechuguilla ^ gave lechuguilla), vegetable oils (Oregano Lippiagraveolens), derivatives of animal origin (Chitosan hydrolyzate and macarela oilDecapterusmuroadsi), natural additives and bacteria of the genus Bacillus.
On the other hand, US Pat. No. 5,888,501, which consists of a biologically active formulation that induces systemic immunity on the surface of the plant, which diffuses in its natural openings to induce resistance to phytopathogens (Bacteria such as Erwiniatracheiphila and the Mozaic virus) in plants of cabbage, cucumber and tomato, controlling the development of the disease, where the cause of immunity is to impart the bacterial proteins of Xanthomonascampestris pv malvacearum inside the destination plant. However, the present invention deals with a bactericidal and resistance-inducing composition as an organic treatment for preventing, controlling and / or eliminating diseases caused by bacteria of the genus XErwinia, Pseudomonas, RalstoniaXanthomona, Clavibacter, Acidovorax, Agrobacterium, CurtobacteriumStreptomyces, Actinobacteria, SpiroplasmasyFitoplasmas in agricultural crops, through the use of plant extracts (Alliumsativumüesco garlic, Equinacia angustifolia, Cyrillic seedCzYrz¿y paradisiaca, Larrea tridentata, pollen-propóleo, flower of JamaicsLHibiscussabdariffa, marine algae, tepezcohuite zwaya tenuiflora, guiche de lechuguilla ígave lechuguilla), vegetable oils (OiéganoLippiagraveolens), derivatives of animal origin (Chitosan hydrolyzate and macarela oilDecapterusmuroadsi), natural additives and bacteria of the genus Bacillus.
As well as, the international patent WO1999053761 Al, which consists of a method to induce antifungal resistance, antibacterial Erwiniaamylovora (bacterial fire), Xanthomonascampestris (bacterial blotch of trees), Pseudomonasyringae (bacterial blight, rice and cereals) and antiviral in plants. The present invention consists of a bactericidal and resistance-inducing composition of organic origin, to prevent, control and / or eliminate diseases caused by bacteria of the genus Erwinia, Pseudomonas, RalstoniaXanthomona, Clavibacter, Acidovorax,
Agrobacterium, CurtobacteriumStreptomyces, Actinobacteria, Spiroplasmas andFitoplasmas in agricultural crops, through the use of plant extracts (A oAlliumsativum fresh, Equinacia angustifolia, grapefruit seedCzYrws paradisiaca, Larrea tridentata, pollen-propóleo, flower of JamaicsJiibiscussabdariffa, seaweed, tepezcohuite z / Hosa tenuiflora, guiche de lechuguilla / 4gave lechuiguillá), vegetable oils (OréganoLippiagraveolens), derivatives of animal origin (Chitosan hydrolyzate and macarela oilDecapterusmuroadsi), natural additives and Bacillus bacteria.
The global tendency to find products of natural origin for the control of diseases caused by phytopathogenic bacteria, the search for strategies, techniques and methods that increase agricultural productivity and maintain the ecological balance without harming ecosystems and without risking human health, constitutes today in day a great challenge for agriculture and its development (Gallegos et al, 2004).
Currently a line of organic products is growing widely, such products are losbioinsumos, which are oriented not only to the control of weeds, pests and diseases, but also to the contribution of nutrients to plants during the different phases of their life cycle.
So, the production of bio-inputs represents a novel industry, where biocides (bioinsecticides, biofungicides, biobactericides, etc.) and biofertilizers are discussed. Thus, a bioinsumer is the extracts, oils, vegetable gums, etc., for the control of pests and diseases. In this regard, there is a large amount of this type of products, which
They are used for the control of different phytosanitary problems in various agricultural crops.
Faced with this situation, a new approach to combat phytopathogenic bacteria tries to integrate new options aimed at protecting and increasing harvests but with non-harmful alternatives; in this case the use of substances of natural origin, which the plants themselves have developed through evolution can be an alternative to limit the use of agrochemicals little by little. This could eliminate several adverse effects caused by the use of synthetic compounds due to the rapid biodegradability of organic metabolites, since these easily disappear from the air and soil environment after they are applied in the field (Tanaka andAndomuro, 1993) .
That is why the development of bactericidal formulations of organic origin for the control of bacteria in agricultural crops, represent a great alternative, which is why we are working on this, highlighting the use of bio-inputs, alone or in combination, in addition to the incorporation of other components, such as microorganisms and / or mineral salts, that promote the induction of resistance in plants.
Whose purpose is to provide products with a synergistic operation in a natural way, for the prevention and / or control of bacteria, besides inducing systemic resistance in the plants, to promote the control of the disease and above all to have a lower environmental impact; Therefore, some of the most relevant compositions and / or methods related to this area are mentioned, such as the United States Patent
North American US 5, 698, 200, which deals with compositions and methods for the control of diseases caused by phytopathogenic bacteria of the genus Erwinia, Pseudomonas, Xanthomonas, Agrobacterium, or Clavibacter, is preferably a bacteria of the soft rot Erwiniagenum in plants, based on a concentrated and clarified malted cereal grain extract. On the other hand, the present novelty emphasizes a bactericidal and resistance-inducing composition as an organic treatment to prevent, control and / or eliminate diseases caused by bacteria of the genus Erwinia, Pseudomonas, RalstoniaXanthomona.Clavibacter, Acidovorax,
Agrobacterium, CurtobacteriumStreptomyces, Actinobacteria, Spiroplasmas, andFitoplasmas n agricultural crops, generally of vegetables, through the use of plant extracts (A.}. Or freshAlliwnsativum, Equinacia angustifolia, grapefruit seedChicas paradisiaca, Larrea tridentata, pollen-propóleo, flower of Jam &icsJíibiscussabdariffa, marine algae, tepezcohuite / wosa tenuiflora, guiche de lechuguilla ^ g ve lechuguilla), vegetable oils (OréganoLippiagraveolens), derivatives of animal origin (Chitosan hydrolyzate and macareleu decapterusmuroadsi oil), natural additives and Bacillus bacteria.
U.S. Patent US 7,582, 318, which mentions the use of a natural composition based on Chilean monofloral honey extracts of native plant species to control bacterial infection in plants. By way of example, the composition of the present invention showed to be especially useful for the control of soft rot caused by Erwinia in potatoes, vegetables and flowers in general. The present novelty consists of a bactericidal and resistance-inducing composition as an organic treatment, to prevent, control and / or eliminate diseases caused by bacteria of the genus Erwinia, Pseudomonas, RalstoniaXanthomona, Clavibacter, Acidovorax, Agrobacterium, CurtobacteriumStreptomyces, Actinobacteria, Spiroplasmas andFitoplasmas in agricultural crops. , generally of vegetables, through the use of vegetable extracts (Garlic Alliumsativumüesco, Equinacia angustifolia, seed of grapefruit C / írwí paradisiaca, Larrea tridentata, pollen-propóleo, flower of JamaicaHibiscussabdariffa, marine algae, tepezcohuite / rcostf tenuiflora, guiche de lechuguilla lg ve lechuguilla ), vegetable oils (OréganoLippiagraveolens), derivatives of animal origin (Chitosan hydrolyzate and oil of macaie \? LDecapterusmuroadsi), natural additives and bacteria of the Bacillus genus.
The Brazilian patent BR P 10802223 A2, which deals with an agricultural composition based on vegetable essential oils for the control of pests and diseases in tomato and potato plants. However, the present invention consists of a bactericidal and resistance-inducing composition as an organic treatment, to prevent, control and / or eliminate diseases caused by bacteria of the genus Erwinia, Pseudomonas, RalstoniaXanthomona, Clavibacter, Acidovorax, Agrobacterium, CurtobacteriumStreptomyces, Actinobacteria, Spiroplasmas andFitoplasmas. agricultural crops, generally of vegetables, through the use of vegetable extracts (Alliumsativumñ sco, Equinacia angustifolia, grapefruit seed, Citrus paradisiaca, Larrea tridentata, pollen-propóleo, flower of Ja aicaHibiscussabdariffa, seaweed, tepezcohuite z'mosa tenuiflora, guiche de lechuguilla fgave lechuguilla), vegetable oils (OréganoLippiagraveolens), animal derivatives (Chitosan hydrolyzed and macarQlaDecapterusmuroadsi oil), natural additives and Bacillus bacteria.
And another relevant bactericide, is the one developed in 2008 by the Council of Agriculture (COA, acronym in English), which consists of an organic bactericide for agricultural crops, from extracts of plants with secondary metabolites, such as alkaloids, glycosides, phenols, sterols and essential oils, which are used by plants as defense mechanisms against predators, parasites and diseases. The organic bactericide can effectively fight more than 10 types of fungi and bacteria, including Bacilluscereus and Escherichiacoli. However, the present invention deals with a bactericidal and resistance-inducing composition as an organic treatment, for preventing, controlling and / or eliminating diseases caused by bacteria of the genus Erwinia, Pseudomonas, RalstoniaXanthomona, Clavibacter, Acidovorax,
Agrobacterium, CurtobacteriumStreptomyces, Actinobacteria, Spiroplasmas andFitoplasmas in agricultural crops, generally of vegetables, through the use of plant extracts (A) or freshAUiumsativum fresh, Equinacia angustifolia, grapefruit seed Trws paradisiaca, Larrea tridentata, pollen-propolis, flower of ís &hibiscussabdariffa, algae marinas, tepezcohuite zwosa tenuiflora, guiche de lechuguilla ^ g ve lechuguilla), vegetable oils (OréganoLippiagraveolens), derivatives of animal origin (Chitosan hydrolyzate and macaida oilDecapterusmuroadsi), natural additives and Bacillus bacteria. In itself, there is no information on the use of bactericidal formulas, based on vegetable extracts-vegetable oils-microorganisms, with a synergy that distinguishes them, as is the case of the present invention, a product that in this document is identified as innovative.
The world demand for an organic-based production that allows a supply outside of agrochemicals and secondly the knowledge that plant molecules have bioactive characteristics of sanitary control; which, when combined with microbiological based control, generated a combination of greater efficiency against fungi, diseases and pests.
So in this frame of reference, the present novelty, solves the previously described problems, representing the effort made in the search for technological innovation; highlighting the use of bio-inputs, such as extracts and vegetable oils, as well as derivatives of animal origin, as well as the incorporation of bacteria of the genus Bacillussp., which in isolation do not represent a solution to the diseases of agricultural crops; however, with the synergy found among these components, as a result of laboratory and field evaluations, they present bactericidal activity, in addition to nourishing and inducing systemic resistance to the plant for the control and prevention of diseases in agricultural crops, so that the efficiency and effectiveness of this innovation have been achieved for the benefit of organic agriculture, human health and the environment in general.
BRIEF DESCRIPTION OF THE FIGURES
Figure 1. Flow diagram of elaboration of the bactericidal composition (Biobacter O). Figure 2. Graph of the effect of the bactericidal composition on the percentage of inhibition of the growth of phytopathogenic bacteria of agricultural crops of commercial importance. The bactericidal activity is observed before Acidovorax (100%), Clavibacter (\ QQ%), Erwinia (\ 00%), Pse domonas (T5%), Ralstonia (75%) and Xanthomonas (\ 00%).
Figure 3. Percentage of inhibition of (a) Acidovorax control, with respect to (b) bactericidal composition, (c) Clavibacter control - with respect to (d) bactericidal composition, (e) Erwiniacon control with respect to (f) bactericidal composition. (g) Pseudomonas control with respect to (h) bactericidal composition, (i) Ralstonia with respect to j) bactericidal composition (k) Xanthomonas with respect to (1) bactericidal composition. Rectifying in a visual way the potential of in vitro effectiveness of the bactericidal composition, before these bacteria of agricultural importance.
Figure 4. In vivo bactericidal effectiveness of the bactericidal composition against phytopathogenic bacteria of the Clavibacter genus in tomato culture. On the left side, the control treatment is shown, so tomato plants with damage caused by Clavibacter are observed; instead of the right side, the treatment of tomato plants with the bactericidal composition (Biobacter O) is shown, so there is no damage caused by Clavibacter in the tomato plants.
DETAILED DESCRIPTION OF THE INVENTION
Below is detailed the bactericidal composition for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, highlighting the use, bactericidal and / or controlling effect and the synergy of its components that harmoniously and efficiently integrate one or more: (a) vegetable extracts, (b) vegetable oils, (c) derivatives of animal origin, (d) microorganisms, (e) organic emulsifiers and acidifiers and (f) conditioners and / or organic diluents.
The component (a) corresponds to:
Aqueous extracts
Flower of JamaicaHibiscussabdariffa
Equinacea angustifolia
Citrus paradisiaca grapefruit seeds
Seaweed
Governor Larrea tridentata
Guiche de lechuguilla / ígave lechuguilla
Extractosetanolics
Garlic Alliumsativum
Tepezcohuite zmosa tenuiflora
Pollen-propolis
The component (b) corresponds to:
Oregano essential oil Lippiagraveolens
The component (c), is constituted by:
Chitosanhydrolyzed
Mackerel oil Decapterusmuroadsi
The component (d), corresponds to:
Bacteria of the genus Bacillus
The component (e), comprises:
Soy lecithin
Apple vinager
The component (f), comprises:
Calcium silicate
Potassium sorbate
Methyl salicylate
Raw water
Ethyl alcohol
As can be seen, the present invention is a complex of organic molecules, which represents an alternative with high bactericidal and bacteriostatic action potential at the cellular level, preventing the formation of membranes and blocking oxidative phosphorylation, for obtaining energy, so that It has a broad spectrum of control and works through several modes and mechanisms of action, directly influencing both gram-positive and gram-negative bacteria, inhibiting its development and / or eliminating its larger population.
It also represents a powerful antiseptic, since it causes the cell lysis of bacteria, eliminating the selection of resistant bacterial strains. In addition to inducing systemic resistance at the physiological level in plants, strengthening organs susceptible to damage, ensuring effective control by nullifying or significantly reducing the possibility of evolution of bacterial resistance, due to the integral action of the product.
In summary, the advantages or benefits of the object of the invention are presented as follows:
- 100% organic product. Non-toxic to humans, animals and plants.
Bacteriostatic and bactericidal broad-spectrum control, against gram-positive and gram-negative bacteria of the genus Pseudomonas, Acidovorax, Erwinia, Clavibacter, Xanthomona, Agrobacterium, Ralstonia, Curtobacterium, Streptpmyces, Actinobacteria, Mycoplasma, Spiroplasma and Fitopl sma in agricultural crops.
- Induces systemic resistance acquired in plants, against the aforementioned phytopathogenic bacteria, and other microorganisms.
- Acts immediately and effectively.
- It has durable protection.
- Strengthens susceptible organs in the plant.
- Rapid damage healing
To obtain approximately 1 L of the bactericidal composition, it is indicated below by a numerological order ascending from 1 to 15, each of the steps for the incorporation of each of the components, as shown in Figure 1.
First, the preparation of an initial mixture is carried out:
In a metal vessel with constant agitation with the help of a propeller mechanical stirrer, 55-225mL, preferably 110mL of (1) raw water, 85-345mL, preferably 170mL of (2) aqueous extract are added and mixed first. of iamaica flower Hibiscussabdariffa (in a ratio of 1: 10), 25-105mL, preferably 50 mL of (3) aqueous extract of Equinacea angustifolia, 5-25mL, preferably 10 mL of (4) aqueous extract of grapefruit seedsCi / ms paradisiacal, 25-105mL, preferably 50mL of (5) seaweed extract, 140-565mL, preferably 280mL of (6) culture of the Bacillussubtilis strain, 15-65mL, preferably 30mL of (7) extract of guiche de lechuguilla4gave lechuguilla, slowly add 25-105 gr, preferably 50 gr of (8) calcium silicate until its dissolution during a time of 5-10 minutes, preferably 10 minutes and finally slowly add 5-25 g, preferably 10 g. gr of (9) potassium sorbate until its dissolution for a time of 3-5 minutes, preferably 5 minutes.
To then carry out the realization of the following premixes:
Realization of premixes. Three premixes were made separately in metal containers with constant agitation by mechanical depropela ungitator, which correspond to:
(10) Premix 1, add and mix 5-25mL, preferably 10mL of (1) ethanolic extract of fresh Alliumsativum garlic, 5-25mL, preferably 10mL of (2) ethanol extract of tepezcohuite / mosa tenuiflora, 10-45mL , preferably 20 mL of (3) pollen-propolis ethanolic extract filtered in a ratio of 1: 10-1: 30 (w / v), preferably 1: 20 (w / v) and slowly 0.25-1.5 g, preferably 0.5. g of (4) methyl salicylate until dissolved for a time of 5-8 minutes, preferably 8 minutes.
(11) Premix 2, 50-205 ml, preferably 100 ml of (1) ethyl alcohol are added and mixed and slowly 5-25 ml, preferably 10 ml of (2) chitosan hydrolyzate are added until its incorporation for a time of 3- 6 minutes, preferably 5 minutes.
(12) Premix 3, add and mix 25-105mL, preferably 50mL of (l) oil of ovéganoLippiagraveolens, 10-45mL, preferably 20mL of (2) acax oil from Decapterusmuroadsi and 15-65mL, preferably 30mL of ( 3) soy lecithin with a stirring period of 3-5 minutes, preferably 5 minutes.
Subsequently, a final mixture is made, which is done in the following manner, in a metal vessel with constant agitation with the help of mechanical depropela agitator, add and mix 40-165mL, preferably 80 mL of (13) vinegar of apple and 15-65mL, preferably 30 mL of (14) governor extract JLarrea tridentata.After the initial mixture, premix 1, premix 2, premix 3 and the final mixture, these are incorporated in the following order: initial mixture , premix 1, premix 2, premix 3 and final mixture, finally in a metal vessel with constant agitation with the help of a propeller, subjecting the entire formulation to agitation for a period of 15-30 minutes, preferably 25 minutes. To later pack, label and pack the product.
To determine the bactericidal efficiency of the present invention, against phytopathogenic bacteria that attack agricultural crops; As well as its physicochemical characteristics, the following tests were carried out:
Example 1. In vitro bactericidal activity against phytopathogenic bacteria from agricultural crops. The bactericidal composition was evaluated against gram-positive and gram-negative bacteria of the genotype Clovibacter, Agrobacterium, Erwinia, PseudomonasJCantomonas and Ralstonia, the results of which are shown in Figure 3. The technique of poisoned medium was used, for which nutritive agar was used as a culture medium. , to which the antibacterial composition was added, adjusting to a concentration of 0.8 mL of the composition / 200 mL of culture medium, for later evaluation with each of the phytopathogenic bacteria. Firstly, 100 μl of the bacterial suspension adjusted to 1 x 10"6 CFU are dispersed in the culture medium and incubated at 28 ° C for 24-72 hours.The number of colonies is quantified and the percentage of inhibition is estimated. considering the number of control colonies (CFU) (0 mg / L) as 0% inhibition as shown in Figure 2.
Example 2. Bactericidal activity in vivo against phytopathogenic bacteria of agricultural crops. The bactericidal composition was evaluated against gram-positive bacteria of genQToClavibacter, in a commercial tomato culture, the results of which are shown in Figure (4). Two treatments were evaluated: Treatment 1: Cultivation of tomato with Clavibacter without the bactericidal composition (Biobacter O) as a control; Treatment 2: Tomato culture with Clavibacter plus the bactericidal composition (Biobacter O). Two applications were made by foliar and irrigation system, of the bactericidal composition in both treatments, each application was made in an interval of 4 days. The foliar sampling of the treatment with the bactericidal composition was carried out 7 days after the last application, in which 5 random samples of plant tissue were taken with minimal symptoms, strong damage and apparently healthy leaves, as well as the control . The results of the phytopathological analysis of the vegetable tissues of the tomato crop were determined by MASTERLAB Laboratories, using the following methodology in a very general way: they were planted in culture medium for AN bacteria, later a presumptive test was carried out in specific NBY medium and The gram test, KOH 3%, was determined. Where two samples were analyzed: M-l Ship 17 (Control) and M-2 Ship 17 (Biobacter O).
The following are the phytopathological results of the tissue of tomato plants: Analysis requested:
Type of sample: Fabric
Cultivation: Tomato
Date: 06/15/2011
Where the sample M-1 Ship 17 (Control) is positive for the presence of Clavibacter and the sample M-2 Ship 17 (Biobacter O) is negative for the presence of Clavibacter, so these results highlight the potential for effectiveness in vivo of the bactericidal composition, before this bacterium of agricultural importance.
Example 3. Physicochemical analysis of the product.
For the physicochemical analysis of the bactericidal composition, the pH was determined by the pHMeterSensIon 3 brand Hatch; the density, by using an OHAUS analytical scale, using a 100 ml volumetric flask. The solubility of the product was based on water at a ratio of 1: 25 (1 ml of sample in 25 ml of water),
Appearance and color were parameters that were determined by visual observation. Below are the results of the physicochemical characteristics of the bactericidal composition, in the following table:
Claims (40)
1. The bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, characterized essentially by comprising one or more: (a) vegetable extracts, (b) vegetable oils, (c) derivatives of animal origin, (d) microorganisms, (e) organic emulsifiers and acidifiers and (f) organic conditioners and / or diluents.
2. The bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 1, characterized in that the plant extracts correspond to aqueous extracts such as the flower of Hibiscus sambar, Hibiscussabdariffa, Equinacea angustifolia and grapefruit seeds Citrus paradisiaca, marine algae, governor Larrea tridentata and guiche de lechuguilla Agave lechuguilla and ethanolic extracts such as garlic Alliumsativum, tepezcohuite Mimosa tenuiflora and pollen-propóleo.
3. The bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 1, characterized in that the vegetable oils correspond to the oregano essential oil Lippiagraveolens.
4. The bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 1, characterized in that the derivatives of animal origin correspond to chitosan hydrolyzate and mackerel oil Decapterusmuroadsi.
5. The bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 4, characterized in that the mackerel oil Decapterusmuroadsi was used as an important source of omega 3 fatty acids.
6. The bactericidal and inducing composition for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 1, characterized in that the microorganisms correspond to the Bacillus strain, specifically Bacillussubtilismulúcepa ..
7. The bactericidal and inducing composition for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 1, characterized in that the emulsifiers and organic acidifiers correspond to soy lecithin and apple cider vinegar, respectively.
8. The bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 1, characterized in that the conditioners and / or organic diluents correspond to the calcium silicate, potassium sorbate, Methyl salicylate, raw water and ethyl alcohol.
9. The bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 1, characterized in that the bactericidal composition is a 100% organic product, non-toxic for humans, animals and plants. .
10. The bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 1, characterized in that the commercial name of the bactericidal composition is Biobacter O.
11. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, characterized in that it comprises the realization and incorporation of an initial mixture, three premixes; premix 1, premix 2, premix 3 and the final mixture, to finally stir the composition at a certain time.
12. Process for the preparation of the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 11, characterized in that for the realization of the initial mixture, it comprises the addition and mixing of the following components: raw water, Hibiscussabdariffa flower extract, Equinacea angustifolia extract, Citrus paradisiaca grapefruit seed extract, seaweed extract, culture of the Bacillussubtilis multicepa strain, lechuguilla guiche extract, Agave lechuguilla, silicate of calcium and potassium sorbate.
13. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 12, characterized in that the initial mixture comprises a volume of 55-225mL, preferably 110 mL of raw water, 85-345 mL, preferably 170 mL of flower extract of jamaicaHibiscussabdariffa in a ratio of 1: 10, 25-105mL, preferably 50mL of extract of Equinacea angustifolia, 5-25mL, preferably 10mL of grapefruit seed extract, 25-105mL, preferably 50mL of seaweed extract, 140 -565mL, preferably 280 mL of culture of the Bacillussubtilismulúcepa strain, 15-65mL, preferably 30 mL of lechuguilla gourd extract Agave lechuguilla, 25-105 g, preferably 50 g of calcium silicate and 5-25 g, preferably 10 gr of potassium sorbate.
14. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 12, characterized in that the calcium silicate is mixed during a period of 5-10. minutes, preferably 10 minutes for its total dissolution.
15. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 12, characterized in that the potassium sorbate is mixed during a period of 3-5 minutes, preferably 5 minutes for its total dissolution.
16. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 11, characterized in that the premix 1 comprises the addition and mixing of the following components : garlic etnic extract Alliumsativ mÍTesco, ethanol extract of tepezcohuite mosa tenuiflora, ethanolic extract of filtered pollen-propolis and methyl salicylate.
17. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 16, characterized in that the premix 1, comprises a volume of 5-25 mL, preferably 10 mL of ethanolic extract of garlic Alliumsativumfresco, 5-25 mL, preferably 10 mL of ethanol extract of tepezcohuite / wosa tenuiflora, 10-45 mL, preferably 20 mL of filtered pollen-propolis ethanolic extract and 0.25-1.5 g, preferably 0.5 methyl salicylate graph.
18. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 17, characterized in that the ratio of the ethanol extract of pollen-propolis is 1: 10 -1: 30 (w / v), preferably 1: 20 (w / v).
19. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 16, characterized in that the methyl salicylate is mixed during a period of 5-8. minutes, preferably 8 minutes for its total dissolution.
20. Process for the preparation of the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 11, characterized in that for the preparation of premix 2, it comprises the addition and mixing of the following components: ethyl alcohol and chitosan hydrolyzate.
21. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 20, characterized in that the premix 2 comprises a volume of 50-205 mL, preferably 100 mL of ethyl alcohol and 5-25 mL, preferably 10 mL of hydrolysed chitosan.
22. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 20, characterized in that the hydrolyzed chitosan is mixed during a period of 3-6 minutes , preferably 5 minutes, until its incorporation into the formulation.
23. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 1, characterized in that for the preparation of the premix 3, it comprises the addition and mixture of the following components: oregano oil Lippiagraveolens, macaque oil Decapterusmuroadsi and soy lecithin.
24. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 23, characterized by pre-mix 3, comprises a volume of 25-105 mL, preferably 50 mL of oregano oil Lippiagraveolens, 10-45 mL, preferably 20 mL of macadam oil Decapterusmuroadsi and 15-65 mL, preferably 30 mL of soy lecithin.
25. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 11, characterized in that for the realization of the final mixture, it comprises the addition and mixing of the following components: apple cider vinegar and governor extract Larrea tridentata, to finally subject the composition to agitation.
26. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 25, characterized in that the final mixture comprises a volume of 40-165 mL, preferably 80 mL of apple vinegar and 15-65 mL, preferably 30 mL of governor extract Larrea tridentata.
27. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 11, characterized in that the incorporation of the mixtures and premixes to formulate the bactericidal composition, it is given in the following order: initial mix, three premixes; premix 1, premix 2, premix 3 and the final mixture.
28. Process for preparing the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 27, characterized in that the formulated bactericidal composition finally comprises a stirring period of 25 hours. minutes
29. The use of the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, characterized in that it is a bactericide and inducer of resistance to prevent, control and / or eliminate phytopathogenic diseases in agricultural crops. , caused by phytopathogenic bacteria positive and gram negative.
30. The use of the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 29, characterized in that the bactericidal composition is generally effective against all types of phytopathogenic bacteria, especially against the genera of Acidovorax, Clavibacter, Erwinia, Pseudomonas, RalstoniaJCanthomonas, Agrobacterium, Streptomyces, Actinob artery, Curtobacterium, Mycoplasma, Spiroplasmas andFitoplasmas.
31. The use of the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 29, characterized in that the in vitro bactericidal activity comprises an effectiveness of 100% and 75%. % inhibition in the growth of phytopathogenic bacteria.
32. The use of the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 31, characterized in that the effectiveness of 100%, comprises the inhibition in the growth of bacteria of the genus Acidovorax, Clavibacter, Erwinia and Xanthomonas.
33. The use of the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 31, characterized in that the effectiveness of 75%, comprises the inhibition in the growth of the bacteria of the genus Pseudomonas and Ralstonia.
34. The use of the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 29, characterized in that the bactericidal activity in vivo comprises an effectiveness of 100% inhibition in the growth of phytopathogenic bacteria.
35. The use of the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 34, characterized in that the effectiveness of 100%, comprises the inhibition of bacteria of the Clavibacter genus .
36. The use of the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 29, characterized in that the use of the bactericidal composition is for all types of agricultural crops. in general.
37. The use of the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 29, characterized in that the use of the bactericidal composition in agricultural crops comprises prevention, control and / or elimination of phytopathogenic diseases.
38. The use of the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 37, characterized in that the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, thanks to the high bactericidal and bacteriostatic potential at the cellular level that presents the bactericidal composition, preventing the formation of membranes and blocking oxidative phosphorylation for obtaining energy, so it has a broad spectrum of control and works through two mechanisms of action, directly affecting all types of gram positive and gram negative bacteria, inhibiting their development and / or eliminating their largest population.
39. The use of the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 38, characterized in that the first mechanism of action of the bactericidal composition consists in causing the lysis Cellular bacteria, thanks to the high content of polyphenolic compounds, eliminating the selection of resistant bacterial strains, as it is also a potent antiseptic.
40. The use of the bactericidal and resistance-inducing composition of organic origin for the prevention, control and / or elimination of phytopathogenic diseases in agricultural crops, according to claim 38, characterized in that the second mechanism of action of the bactericidal composition consists in inducing resistance systemic at a physiological level in plants, strengthening organs susceptible to damage, ensuring effective control, nullifying and / or significantly reducing the possibility of evolution of bacterial resistance, due to the integral action of the composition.
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| ES2628278A1 (en) * | 2016-02-02 | 2017-08-02 | Greencorp Biorganiks De Mexico S.A. De C.V. | Biostimulant formulation of the growth and vegetal development and resistance inductor for the control of diseases caused by phytopathogen viruses and preparation method (Machine-translation by Google Translate, not legally binding) |
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| ES2628278A1 (en) * | 2016-02-02 | 2017-08-02 | Greencorp Biorganiks De Mexico S.A. De C.V. | Biostimulant formulation of the growth and vegetal development and resistance inductor for the control of diseases caused by phytopathogen viruses and preparation method (Machine-translation by Google Translate, not legally binding) |
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