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MX2010002113A - Metodos para detectar oligonucleotidos. - Google Patents

Metodos para detectar oligonucleotidos.

Info

Publication number
MX2010002113A
MX2010002113A MX2010002113A MX2010002113A MX2010002113A MX 2010002113 A MX2010002113 A MX 2010002113A MX 2010002113 A MX2010002113 A MX 2010002113A MX 2010002113 A MX2010002113 A MX 2010002113A MX 2010002113 A MX2010002113 A MX 2010002113A
Authority
MX
Mexico
Prior art keywords
methods
oligonucleotides
detecting
compositions
detecting oligonucleotides
Prior art date
Application number
MX2010002113A
Other languages
English (en)
Inventor
Francois Jean-Charles Natt
Iwan Beuvink
Andrew Geall
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of MX2010002113A publication Critical patent/MX2010002113A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La invención proporciona métodos y composiciones para detectar y/o cuantificar oligonucleótidos de ácidos nucleicos. Estos métodos y composiciones son útiles para detectar y cuantificar los oligonucleótidos objetivo sintéticos de diagnóstico y/o terapéuticos, tales como aptámeros, ARNi, siARN, oligonucleótidos anti-sentido, ó ribozimas en una muestra biológica.
MX2010002113A 2007-08-23 2008-08-21 Metodos para detectar oligonucleotidos. MX2010002113A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95748707P 2007-08-23 2007-08-23
PCT/EP2008/060948 WO2009024599A1 (en) 2007-08-23 2008-08-21 Methods for detecting oligonucleotides

Publications (1)

Publication Number Publication Date
MX2010002113A true MX2010002113A (es) 2010-03-26

Family

ID=40076846

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010002113A MX2010002113A (es) 2007-08-23 2008-08-21 Metodos para detectar oligonucleotidos.

Country Status (9)

Country Link
US (1) US20110097716A1 (es)
EP (1) EP2183390A1 (es)
KR (1) KR20100063079A (es)
CN (1) CN101835903A (es)
AU (1) AU2008290545A1 (es)
BR (1) BRPI0815725A2 (es)
CA (1) CA2695420A1 (es)
MX (1) MX2010002113A (es)
WO (1) WO2009024599A1 (es)

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DE12722942T1 (de) 2011-03-31 2021-09-30 Modernatx, Inc. Freisetzung und formulierung von manipulierten nukleinsäuren
WO2012149042A2 (en) 2011-04-25 2012-11-01 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
JP6184945B2 (ja) 2011-06-08 2017-08-23 シャイアー ヒューマン ジェネティック セラピーズ インコーポレイテッド ｍＲＮＡ送達のための脂質ナノ粒子組成物および方法
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
HRP20220250T1 (hr) 2011-10-03 2022-04-29 Modernatx, Inc. Modificirani nukleozidi, nukleotidi i nukleinske kiseline, te njihove uporabe
SI2791160T1 (sl) 2011-12-16 2022-07-29 Modernatx, Inc. Sestave modificirane MRNA
IL321422A (en) 2012-01-11 2025-08-01 Ionis Pharmaceuticals Inc Compositions and methods for modulation of ikbkap splicing
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US10501512B2 (en) 2012-04-02 2019-12-10 Modernatx, Inc. Modified polynucleotides
AU2013243952A1 (en) 2012-04-02 2014-10-30 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
SMT202200337T1 (it) 2012-11-26 2022-09-14 Modernatx Inc Rna modificato al livello del terminale
CN105026578B (zh) * 2013-03-05 2019-03-15 安捷伦科技有限公司 通过引物延伸合成探针库
WO2014145620A2 (en) * 2013-03-15 2014-09-18 The Broad Institute, Inc. Novel hybridization probes and uses thereof
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
US10023626B2 (en) 2013-09-30 2018-07-17 Modernatx, Inc. Polynucleotides encoding immune modulating polypeptides
US10323076B2 (en) 2013-10-03 2019-06-18 Modernatx, Inc. Polynucleotides encoding low density lipoprotein receptor
US11162096B2 (en) 2013-10-14 2021-11-02 Ionis Pharmaceuticals, Inc Methods for modulating expression of C9ORF72 antisense transcript
JP6892433B2 (ja) 2015-04-03 2021-06-23 ユニバーシティ・オブ・マサチューセッツＵｎｉｖｅｒｓｉｔｙ Ｏｆ Ｍａｓｓａｃｈｕｓｅｔｔｓ 十分に安定化された非対称ｓｉｒｎａ
WO2017030973A1 (en) * 2015-08-14 2017-02-23 University Of Massachusetts Bioactive conjugates for oligonucleotide delivery
CA2998810A1 (en) 2015-09-17 2017-03-23 Modernatx, Inc. Compounds and compositions for intracellular delivery of therapeutic agents
ES2924407T3 (es) 2015-12-10 2022-10-06 Modernatx Inc Composiciones y procedimientos para el suministro de agentes terapéuticos
SMT202200252T1 (it) 2015-12-22 2022-07-21 Modernatx Inc Composti e composizioni per il rilascio intracellulare di agenti
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
WO2018065622A1 (en) * 2016-10-07 2018-04-12 Secarna Pharmaceuticals Gmbh & Co Kg Immunosuppression-reverting oligonucleotides inhibiting the expression of cd39
US11583504B2 (en) 2016-11-08 2023-02-21 Modernatx, Inc. Stabilized formulations of lipid nanoparticles
US11085071B2 (en) 2017-02-01 2021-08-10 Spot Biosystems Ltd. Highly stable and specific molecular beacons encapsulated in cationic lipoplex nanoparticles and application thereof
AU2018234828A1 (en) 2017-03-15 2019-09-19 Modernatx, Inc. Lipid nanoparticle formulation
EP3596041B1 (en) 2017-03-15 2022-11-02 ModernaTX, Inc. Compound and compositions for intracellular delivery of therapeutic agents
AU2018234814B2 (en) 2017-03-15 2022-06-30 Modernatx, Inc. Crystal forms of amino lipids
WO2018232120A1 (en) 2017-06-14 2018-12-20 Modernatx, Inc. Compounds and compositions for intracellular delivery of agents
WO2018232357A1 (en) 2017-06-15 2018-12-20 Modernatx, Inc. Rna formulations
US11744801B2 (en) 2017-08-31 2023-09-05 Modernatx, Inc. Methods of making lipid nanoparticles
US11279930B2 (en) 2018-08-23 2022-03-22 University Of Massachusetts O-methyl rich fully stabilized oligonucleotides
WO2020061367A1 (en) 2018-09-19 2020-03-26 Modernatx, Inc. Compounds and compositions for intracellular delivery of therapeutic agents
JP7640452B2 (ja) 2018-09-19 2025-03-05 モデルナティエックス インコーポレイテッド 高純度ｐｅｇ脂質及びそれらの使用
WO2020061284A1 (en) 2018-09-19 2020-03-26 Modernatx, Inc. Peg lipids and uses thereof
WO2020061457A1 (en) 2018-09-20 2020-03-26 Modernatx, Inc. Preparation of lipid nanoparticles and methods of administration thereof
JP7491934B2 (ja) * 2019-01-03 2024-05-28 ディーエヌエー スクリプト オリゴヌクレオチドセットのワンポット合成
CA3125441A1 (en) 2019-01-18 2020-07-23 University Of Massachusetts Dynamic pharmacokinetic-modifying anchors
CN109913534A (zh) * 2019-01-24 2019-06-21 中国药科大学 一种基于级联DNA链循环反应和荧光共振能量转移效应的两种miRNA同时检测的方法
CN109868309B (zh) * 2019-03-05 2022-11-11 苏州恩可医药科技有限公司 基于通用碱基替换插入的单链dna扩增方法
CA3149835A1 (en) 2019-08-09 2021-02-18 University Of Massachusetts Chemically modified oligonucleotides targeting snps
US12365894B2 (en) 2019-09-16 2025-07-22 University Of Massachusetts Branched lipid conjugates of siRNA for specific tissue delivery
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CN111500676B (zh) * 2020-04-21 2022-09-09 桂林旅游学院 一种将寡聚核苷酸逆转录成cDNA的方法及寡聚核苷酸检测方法
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ATE480644T1 (de) * 2005-01-25 2010-09-15 Rosetta Inpharmatics Llc Verfahren zur quantifizierung kleiner rna- moleküle

Also Published As

Publication number Publication date
BRPI0815725A2 (pt) 2015-02-10
CA2695420A1 (en) 2009-02-26
WO2009024599A1 (en) 2009-02-26
KR20100063079A (ko) 2010-06-10
US20110097716A1 (en) 2011-04-28
EP2183390A1 (en) 2010-05-12
AU2008290545A1 (en) 2009-02-26
CN101835903A (zh) 2010-09-15

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