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McEachern, 1987 - Google Patents

Regulation of plasmid replication by the interaction of an initiator protein with multiple DNA binding sites

McEachern, 1987

Document ID
15508363570466372465
Author
McEachern M
Publication year

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The Escherichia coli plasmid R6K initiates replication at any of three sites, termed the $\alpha $, $\beta $, and $\gamma $ origins, which are distributed over a 4 kb region. Two R6K encoded regions, the $\gamma $ origin and the structural gene for the $\pi $ protein …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/743—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Agrobacterium; Rhizobium; Bradyrhizobium
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