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Akishev et al., 2003 - Google Patents

Thermostable DNA polymerase from Thermus thermophilus B35: preparation and study of a modified form of the enzyme with high affinity to ddNTP

Akishev et al., 2003

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Document ID
13421015129614522641
Author
Akishev A
Rechkunova N
Tomilova J
Lebedeva N
Lavrik O
Degtyarev S
Publication year
Publication venue
Biochemistry (Moscow)

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Snippet

The hybrid protein consisting of Tte DNA polymerase fragment and mutant Taq DNA polymerase (F667Y) fragment in the ratio 20: 1 was constructed. Affinity of the modified enzyme (substitutions F669Y, V667I, and S692Q) to ddNTP was two orders higher than that …
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