[go: up one dir, main page]

Nagarajan, 2020 - Google Patents

Microengineered hydrogels for spatially-resolved, multiplexed microRNA quantification from tissue

Nagarajan, 2020

View PDF
Document ID
9462713641917855817
Author
Nagarajan M
Publication year

External Links

Snippet

There is increasing evidence that obtaining both spatially-resolved and multiplexed measurements of miRNA is critical for diagnostic and prognostic value of miRNA tests. In this thesis, we developed a new method for making spatially-resolved and multiplexed …
Continue reading at dspace.mit.edu (PDF) (other versions)

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"

Similar Documents

Publication Publication Date Title
US12370538B2 (en) Particle-drop structures and methods for making and using the same
EP3262192B1 (en) Spatially addressable molecular barcoding
US10508299B2 (en) Microfluidic cell trap and assay apparatus for high-throughput analysis
Pregibon et al. Optimization of encoded hydrogel particles for nucleic acid quantification
EP3277843A2 (en) Methods and compositions for combinatorial barcoding
WO2016149418A1 (en) Methods and compositions for labeling targets and haplotype phasing
JP2015519900A (en) Single particle analysis method and single particle isolation method for particle population
Nagarajan et al. Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays
CN107407685A (en) The method of quick accurate distribution, visualization and analysis individual cells
Hu et al. Assessing breast cancer molecular subtypes using extracellular vesicles’ mRNA
Tentori et al. Quantitative and multiplex microRNA assays from unprocessed cells in isolated nanoliter well arrays
Bhadra et al. An overview of challenges associated with exosomal miRNA isolation toward liquid biopsy-based ovarian cancer detection
Xiao et al. Single‐Cell Enzymatic Screening for Epithelial Mesenchymal Transition with an Ultrasensitive Superwetting Droplet‐Array Microchip
Li et al. High-throughput microfluidic single-cell trapping arrays for biomolecular and imaging analysis
Nagarajan Microengineered hydrogels for spatially-resolved, multiplexed microRNA quantification from tissue
US20190154679A1 (en) Apparatuses and methods for cell and tissue assays and agent delivery
WO2015073744A1 (en) Ultrasensitive detection and characterization of clustered kras mutations using peptide nucleic acid clamp pcr in drop-based microfluidics
Wirth Doktors der Naturwissenschaften (Dr. rer. nat.)
Chapin Encoded hydrogel microparticles for high-throughput molecular diagnostics and personalized medicine
US20220127674A1 (en) Methods for detecting circulating stromal cells
Walsh et al. Capture and enumeration of mRNA transcripts from single cells using a microfluidic device
White Development and Application of Microfluidic Single-Cell Polymerase Chain Reaction
Lee Biomolecular Processing and Quantitative Localization of Single Cell Analysis
Class et al. Patent application title: Microfluidic Cell Trap and Assay Apparatus for High-Throughput Analysis Inventors: Carl Lars Genoble Hansen (Vancouver, CA) Michael Vaninsberghe (Vancouver, CA) Adam White (Toronto, CA) Oleh Petriv (Vancouver, CA) Tim Leaver (Vancouver, CA) Anupam Singhal (Mississauga, CA) William Bowden (Vancouver, CA) Veronique Lecault (Vancouver, CA) Dan Da Costa (Vancouver, CA) Leo Wu (Surrey, CA) Georgia Russell (Vancouver, CA) Darek Sikorski (Vancouver, CA)
Hur High-throughput Rare Cell Detection and Separation Using Inertial Microfluidics