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Krzywkowski et al., 2017 - Google Patents

Fidelity of RNA templated end-joining by chlorella virus DNA ligase and a novel iLock assay with improved direct RNA detection accuracy

Krzywkowski et al., 2017

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Document ID
8909011095558136759
Author
Krzywkowski T
Nilsson M
Publication year
Publication venue
Nucleic acids research

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Ligation-based nucleic acid detection methods are primarily limited to DNA, since they exhibit poor performance on RNA. This is attributed to reduced end-joining efficiency and/or fidelity of ligases. Interestingly, chlorella virus DNA ligase (PBCV-1 DNA ligase) has recently …
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