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Stirling et al., 2017 - Google Patents

Rational design of evolutionarily stable microbial kill switches

Stirling et al., 2017

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Document ID
8706336754567992949
Author
Stirling F
Bitzan L
O’Keefe S
Redfield E
Oliver J
Way J
Silver P
Publication year
Publication venue
Molecular cell

External Links

Snippet

The evolutionary stability of synthetic genetic circuits is key to both the understanding and application of genetic control elements. One useful but challenging situation is a switch between life and death depending on environment. Here are presented" essentializer" and" …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
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