[go: up one dir, main page]

Samuels, 1995 - Google Patents

Electrotransformation of the spirochete Borrelia burgdorferi

Samuels, 1995

View PDF
Document ID
4329657710171026818
Author
Samuels D
Publication year
Publication venue
Electroporation protocols for microorganisms

External Links

Snippet

Borrelia burgdorferi is an etiologic agent of Lyme disease, the most common arthropod- borne disease in the United States (,). The bacterium, a member of the spirochete phylum, has a genome predominantly composed of linear DNA molecules (,). Formulating a medium …
Continue reading at pmc.ncbi.nlm.nih.gov (PDF) (other versions)

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving viable micro-organisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving viable micro-organisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor

Similar Documents

Publication Publication Date Title
Samuels Electrotransformation of the spirochete Borrelia burgdorferi
Kiga et al. Development of CRISPR-Cas13a-based antimicrobials capable of sequence-specific killing of target bacteria
Djordjevic et al. Genetics of human cell lines: III. Incorporation of 5-bromo-and 5-Iododeoxyhridine into the deoxyribonucleic acid of human cells and its effect on radiation sensitivity
Dougherty et al. Specificity of DNA uptake in genetic transformation of gonococci
Catlin et al. The type b capsulation locus of Haemophilus influenzae: map location and size
Naor et al. A halocin-H4 mutant Haloferax mediterranei strain retains the ability to inhibit growth of other halophilic archaea
Roberts et al. [6] Gene transfer in bacterial biofilms
Zegeye et al. Efficient electrotransformation of Bacillus thuringiensis for gene manipulation and expression
Gutiérrez et al. Adaptive resistance mutations at suprainhibitory concentrations independent of SOS mutagenesis
Mauti et al. Molecular identification of soil bacteria by 16srDNA sequence
Brown Volume I
Riley et al. Culture of Escherichia coli and related bacteria
US11692159B2 (en) Growth of bacterial host cells in gas permeable low-density polyethylene bags for production of plasmid DNA or recombinant molecules
Riley et al. Culture of Escherichia coli and related bacteria
Samuels of the Spirochete Borrelia burgdorferi
Perry et al. Isolation and confirmation of Yersinia pestis mutants exempt from select agent regulations
Singh et al. Practical Techniques in Molecular Biotechnology
Liu et al. Genetic Engineering of Bacteriophage Using CRISPR-Cas12a
RU2564120C2 (en) STRAIN OF Escherichia coli BACTERIA - PRODUCENT OF HEAT SHOCK PROTEIN 70 AND METHOD FOR OBTAINING PREPARATION OF HUMAN HEAT SHOCK PROTEIN
Pappas et al. Creating a library of random transposon mutants in Leptospira
Kadono et al. Effect of symbiotic algae on the growth kinetics in dark-grown Paramecium bursaria
Schorr et al. Production of plasmid DNA in industrial quantities according to cGMP guidelines
Delpech Using Vibrio natriegetis for studying bacterial population growth, artificial selection, and the effects of UV radiation and photo-reactivation
Sattley et al. Bacteriology
Giri et al. Heat shock-mediated transformation in several Gram-negative bacteria such as Ralstonia pseudosolanacearum, Pseudomonas aeruginosa, Pseudomonas putida, and Enterobacter roggenkampii