[go: up one dir, main page]

WO2006086111A3 - Reagents for the detection of protein phosphorylation in leukemia signaling pathways - Google Patents

Reagents for the detection of protein phosphorylation in leukemia signaling pathways Download PDF

Info

Publication number
WO2006086111A3
WO2006086111A3 PCT/US2006/000979 US2006000979W WO2006086111A3 WO 2006086111 A3 WO2006086111 A3 WO 2006086111A3 US 2006000979 W US2006000979 W US 2006000979W WO 2006086111 A3 WO2006086111 A3 WO 2006086111A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
protein
reagents
kinases
phosphorylation
Prior art date
Application number
PCT/US2006/000979
Other languages
French (fr)
Other versions
WO2006086111A2 (en
Inventor
Valerie Goss
Albrecht Moritz
Ting-Lei Gu
Kimberly Lee
Roberto Polakiewicz
Original Assignee
Cell Signaling Technology Inc
Valerie Goss
Albrecht Moritz
Ting-Lei Gu
Kimberly Lee
Roberto Polakiewicz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cell Signaling Technology Inc, Valerie Goss, Albrecht Moritz, Ting-Lei Gu, Kimberly Lee, Roberto Polakiewicz filed Critical Cell Signaling Technology Inc
Priority to EP06718094A priority Critical patent/EP2126580A4/en
Publication of WO2006086111A2 publication Critical patent/WO2006086111A2/en
Priority to US11/973,019 priority patent/US20090142777A1/en
Publication of WO2006086111A3 publication Critical patent/WO2006086111A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57426—Specifically defined cancers leukemia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Oncology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Hospice & Palliative Care (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention discloses 424 novel phosphorylation sites identified in signal transduction proteins and pathways underlying human Leukemia, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Adaptor/Scaffold proteins, Cytoskeletal proteins, Cellular Metabolism enzymes, G Protein/GTPase Activating/Guanine Nucleotide Exchange Factor proteins, Immunoglobulin Superfamily proteins, Inhibitor proteins, Lipid Kinases, Nuclear DNA Repair/RNA Binding/Transcription proteins, Serine/Threonine Protein Kinases, Tyrosine Kinases, Protein Phosphatases, and Translation/Transporter proteins.
PCT/US2006/000979 2005-02-10 2006-01-12 Reagents for the detection of protein phosphorylation in leukemia signaling pathways WO2006086111A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP06718094A EP2126580A4 (en) 2005-02-10 2006-01-12 Reagents for the detection of protein phosphorylation in leukemia signaling pathways
US11/973,019 US20090142777A1 (en) 2006-01-12 2007-10-05 Reagents for the detection of protein phosphorylation in leukemia signaling pathways

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65158305P 2005-02-10 2005-02-10
US60/651,583 2005-02-10

Publications (2)

Publication Number Publication Date
WO2006086111A2 WO2006086111A2 (en) 2006-08-17
WO2006086111A3 true WO2006086111A3 (en) 2009-04-16

Family

ID=36793552

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/000979 WO2006086111A2 (en) 2005-02-10 2006-01-12 Reagents for the detection of protein phosphorylation in leukemia signaling pathways

Country Status (2)

Country Link
EP (1) EP2126580A4 (en)
WO (1) WO2006086111A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8227202B2 (en) 2008-07-10 2012-07-24 Nodality, Inc. Methods for diagnosis, prognosis and methods of treatment
US8273544B2 (en) 2008-07-10 2012-09-25 Nodality, Inc. Methods for diagnosis, prognosis and methods of treatment
US9182385B2 (en) 2007-08-21 2015-11-10 Nodality, Inc. Methods for diagnosis, prognosis and methods of treatment

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006320699A1 (en) * 2005-12-01 2007-06-07 New York Blood Center, Inc. Peptide inhibitors of ABL kinases
WO2008153636A1 (en) * 2007-05-04 2008-12-18 Cellsignaling Technology, Inc. Phospho-specific antibodies to p13k regulatory subunit and uses thereof
CN103038248B (en) 2010-05-31 2016-08-10 伦敦健康科学研究中心有限公司 RHAMM binding peptide
DE102010033575B4 (en) * 2010-08-02 2016-01-14 Eberhard-Karls-Universität Tübingen Universitätsklinikum ASPP2 splice variant
GB201322574D0 (en) * 2013-12-19 2014-02-05 Equigerminal Sa Retroviral peptides
EP3877411A4 (en) * 2018-11-05 2022-09-28 The Brigham and Women's Hospital, Inc. Nedd9 in pulmonary vascular thromboembolic disease

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4599305A (en) * 1984-07-16 1986-07-08 The Regents Of The University Of California Method and composition for detection of human chronic myelogenous leukemia
US5667981A (en) * 1994-05-13 1997-09-16 Childrens Hospital Of Los Angeles Diagnostics and treatments for cancers expressing tyrosine phosphorylated CRKL protein

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7183385B2 (en) * 2002-02-20 2007-02-27 Cell Signaling Technology, Inc. Phospho-specific antibodies to Flt3 and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4599305A (en) * 1984-07-16 1986-07-08 The Regents Of The University Of California Method and composition for detection of human chronic myelogenous leukemia
US5667981A (en) * 1994-05-13 1997-09-16 Childrens Hospital Of Los Angeles Diagnostics and treatments for cancers expressing tyrosine phosphorylated CRKL protein

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2126580A4 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9182385B2 (en) 2007-08-21 2015-11-10 Nodality, Inc. Methods for diagnosis, prognosis and methods of treatment
US8227202B2 (en) 2008-07-10 2012-07-24 Nodality, Inc. Methods for diagnosis, prognosis and methods of treatment
US8273544B2 (en) 2008-07-10 2012-09-25 Nodality, Inc. Methods for diagnosis, prognosis and methods of treatment
US8399206B2 (en) 2008-07-10 2013-03-19 Nodality, Inc. Methods for diagnosis, prognosis and methods of treatment
US8778620B2 (en) 2008-07-10 2014-07-15 Nodality, Inc. Methods for diagnosis, prognosis and methods of treatment
US9500655B2 (en) 2008-07-10 2016-11-22 Nodality, Inc. Methods for diagnosis, prognosis and methods of treatment

Also Published As

Publication number Publication date
EP2126580A2 (en) 2009-12-02
EP2126580A4 (en) 2010-11-10
WO2006086111A2 (en) 2006-08-17

Similar Documents

Publication Publication Date Title
WO2007027906A3 (en) Reagents for the detection of protein phosphorylation in leukemia signaling pathways
WO2006086111A3 (en) Reagents for the detection of protein phosphorylation in leukemia signaling pathways
WO2007027957A3 (en) Reagents for the detection of protein phosphorylation in leukemia signaling pathways
WO2007027867A3 (en) Reagents for the detection of protein phosphorylation in carcinoma signaling pathways
US11340232B2 (en) Peptide constructs and assay systems
WO2007133702A3 (en) Reagents for the detection of protein acetylation signaling pathways
JP2019176878A (en) Compositions for dna-protein complex
WO2007127335A3 (en) Reagents for the detection of protein phosphorylation in atm and atr kinase signaling pathways
WO2008009002A3 (en) Reagents for the detection of protein phosphorylation in signaling pathways
WO2008009004A3 (en) Reagents for the detection of protein phosphorylation in signaling pathways
WO2000054046A3 (en) Universal protein array system
WO2003062462A3 (en) Method for isolating nucleic acids and protein from a single sample
WO2004005319A3 (en) Microarrays on mirrored substrates for performing proteomic analyses
WO2001094946A3 (en) Microarrays for performing proteomic analyses
WO2006031800A3 (en) Reverse transfection of cell arrays
WO2008008998A3 (en) Reagents for the detection of protein phosphorylation in signaling pathways
WO2012050963A2 (en) Agents providing controls and standards for immuno-precipitation assays
Arguello et al. In vitro selection with a site-specifically modified RNA library reveals the binding preferences of N6-methyladenosine reader proteins
WO2007085923A3 (en) Novel fluorescent proteins and methods for using same
WO2008009000A3 (en) Reagents for the detection of protein phosphorylation in signaling pathways
WO2009003952A3 (en) Column and method for preparing a biological sample for protein profiling
WO2008140472A3 (en) Functionalized molecules comprising an autosilification moiety and methods of making and using same
WO2000022166A3 (en) Genes for assessing cardiovascular status and compositions for use thereof
WO2007133689A3 (en) Reagents for the detection of protein acetylation signaling pathways
WO2007133688A3 (en) Reagents for the detection of tyrosine phosphorylation in brain ischemia signaling pathways

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2006718094

Country of ref document: EP