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WO2003035829A3 - Nanoparticles having oligonucleotides attached thereto and uses therefor - Google Patents

Nanoparticles having oligonucleotides attached thereto and uses therefor Download PDF

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Publication number
WO2003035829A3
WO2003035829A3 PCT/US2002/032088 US0232088W WO03035829A3 WO 2003035829 A3 WO2003035829 A3 WO 2003035829A3 US 0232088 W US0232088 W US 0232088W WO 03035829 A3 WO03035829 A3 WO 03035829A3
Authority
WO
WIPO (PCT)
Prior art keywords
nanoparticles
methods
nucleic acid
provides
oligonucleotides
Prior art date
Application number
PCT/US2002/032088
Other languages
French (fr)
Other versions
WO2003035829A2 (en
Inventor
So-Jung Park
Thomas A Taton
Chad A Mirkin
Robert L Letsinger
Robert C Mucic
James J Storhoff
Robert Elghanian
Viswanadham Garimella
Zhi Li
Gang Lu
Original Assignee
Nanosphere Inc
So-Jung Park
Thomas A Taton
Chad A Mirkin
Robert L Letsinger
Robert C Mucic
James J Storhoff
Robert Elghanian
Viswanadham Garimella
Zhi Li
Gang Lu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/008,978 external-priority patent/US6984491B2/en
Application filed by Nanosphere Inc, So-Jung Park, Thomas A Taton, Chad A Mirkin, Robert L Letsinger, Robert C Mucic, James J Storhoff, Robert Elghanian, Viswanadham Garimella, Zhi Li, Gang Lu filed Critical Nanosphere Inc
Priority to CA2463323A priority Critical patent/CA2463323C/en
Priority to JP2003538330A priority patent/JP4347049B2/en
Priority to AU2002363062A priority patent/AU2002363062B2/en
Priority to EP02799155A priority patent/EP1478774A4/en
Publication of WO2003035829A2 publication Critical patent/WO2003035829A2/en
Publication of WO2003035829A3 publication Critical patent/WO2003035829A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
PCT/US2002/032088 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor WO2003035829A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA2463323A CA2463323C (en) 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor
JP2003538330A JP4347049B2 (en) 2001-10-09 2002-10-08 Nanoparticles with oligonucleotides attached and methods of use
AU2002363062A AU2002363062B2 (en) 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor
EP02799155A EP1478774A4 (en) 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32786401P 2001-10-09 2001-10-09
US60/327,864 2001-10-09
US10/008,978 2001-12-07
US10/008,978 US6984491B2 (en) 1996-07-29 2001-12-07 Nanoparticles having oligonucleotides attached thereto and uses therefor

Publications (2)

Publication Number Publication Date
WO2003035829A2 WO2003035829A2 (en) 2003-05-01
WO2003035829A3 true WO2003035829A3 (en) 2004-08-26

Family

ID=32684383

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/032088 WO2003035829A2 (en) 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor

Country Status (5)

Country Link
EP (1) EP1478774A4 (en)
JP (1) JP4347049B2 (en)
AU (1) AU2002363062B2 (en)
CA (1) CA2463323C (en)
WO (1) WO2003035829A2 (en)

Cited By (13)

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US6962786B2 (en) 1996-07-29 2005-11-08 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US6986989B2 (en) 1996-07-29 2006-01-17 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US7135055B2 (en) 2001-05-25 2006-11-14 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7208587B2 (en) 1996-07-29 2007-04-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7250499B2 (en) 1996-07-29 2007-07-31 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7253277B2 (en) 2002-07-02 2007-08-07 Nanosphere, Inc. Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
US7985539B2 (en) 2002-05-07 2011-07-26 Northwestern University Nanoparticle probes with raman spectroscopic fingerprints for analyte detection
US11845986B2 (en) 2016-05-25 2023-12-19 Becton, Dickinson And Company Normalization of nucleic acid libraries

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JP4619012B2 (en) * 2004-02-19 2011-01-26 トヨタ自動車株式会社 Construction method of 3D DNA network
ES2242528B1 (en) 2004-03-25 2006-12-01 Consejo Sup. Investig. Cientificas MAGNETIC NANOPARTICLES OF NOBLE METALS.
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JP5398982B2 (en) * 2004-05-24 2014-01-29 ミダテック リミテッド Nanoparticles containing RNA ligands
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GB2525104B (en) 2013-08-28 2016-09-28 Cellular Res Inc Massively Parallel Single Cell Nucleic Acid Analysis
CA2934641A1 (en) * 2013-12-23 2015-07-02 The University Of Queensland Nucleic acid detection method and kit
WO2015159535A1 (en) * 2014-04-16 2015-10-22 富士フイルム株式会社 Detection solution, method for preparing same, and biosensing method
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
JP7508191B2 (en) 2015-03-30 2024-07-01 ベクトン・ディキンソン・アンド・カンパニー Methods and compositions for combinatorial barcoding
CN107580632B (en) 2015-04-23 2021-12-28 贝克顿迪金森公司 Methods and compositions for whole transcriptome amplification
KR102489353B1 (en) 2015-06-01 2023-01-17 캘리포니아 인스티튜트 오브 테크놀로지 Compositions and methods for screening T cells with antigens to specific populations
KR102395450B1 (en) 2015-09-11 2022-05-09 셀룰러 리서치, 인크. Methods and Compositions for Normalizing Nucleic Acid Libraries
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AU2017268369B2 (en) * 2016-05-18 2022-09-01 Integrated Nano-Technologies, Inc. Method for detection of a PCR product
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US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
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US12258613B2 (en) 2017-03-08 2025-03-25 California Institute Of Technology Pairing antigen specificity of a T cell with T cell receptor sequences
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KR20210052389A (en) 2018-08-27 2021-05-10 리제너론 파마슈티칼스 인코포레이티드 Use of Raman spectroscopy in downstream purification
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Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7259252B2 (en) 1996-07-29 2007-08-21 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US8323888B2 (en) 1996-07-29 2012-12-04 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6986989B2 (en) 1996-07-29 2006-01-17 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7250499B2 (en) 1996-07-29 2007-07-31 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6962786B2 (en) 1996-07-29 2005-11-08 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7208587B2 (en) 1996-07-29 2007-04-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6969761B2 (en) 1996-07-29 2005-11-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7135055B2 (en) 2001-05-25 2006-11-14 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7238472B2 (en) 2001-05-25 2007-07-03 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7985539B2 (en) 2002-05-07 2011-07-26 Northwestern University Nanoparticle probes with raman spectroscopic fingerprints for analyte detection
US7253277B2 (en) 2002-07-02 2007-08-07 Nanosphere, Inc. Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
US11845986B2 (en) 2016-05-25 2023-12-19 Becton, Dickinson And Company Normalization of nucleic acid libraries

Also Published As

Publication number Publication date
AU2002363062B2 (en) 2007-03-22
EP1478774A2 (en) 2004-11-24
JP2005525084A (en) 2005-08-25
WO2003035829A2 (en) 2003-05-01
CA2463323A1 (en) 2003-05-01
CA2463323C (en) 2010-12-21
JP4347049B2 (en) 2009-10-21
EP1478774A4 (en) 2006-11-29

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