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WO1999061597A3 - Expression cassette for transformation comprising a modified viral sequence driven by a suitable promoter - Google Patents

Expression cassette for transformation comprising a modified viral sequence driven by a suitable promoter Download PDF

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Publication number
WO1999061597A3
WO1999061597A3 PCT/US1999/011250 US9911250W WO9961597A3 WO 1999061597 A3 WO1999061597 A3 WO 1999061597A3 US 9911250 W US9911250 W US 9911250W WO 9961597 A3 WO9961597 A3 WO 9961597A3
Authority
WO
WIPO (PCT)
Prior art keywords
transformation
expression cassette
suitable promoter
viral sequence
modified viral
Prior art date
Application number
PCT/US1999/011250
Other languages
French (fr)
Other versions
WO1999061597A2 (en
WO1999061597A9 (en
Inventor
Lada Rasochova
Thomas L German
Paul G Ahlquist
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Priority to JP2000550982A priority Critical patent/JP3959965B2/en
Priority to EP99953356A priority patent/EP1086237A2/en
Priority to BR9911065-2A priority patent/BR9911065A/en
Priority to AU43101/99A priority patent/AU763096B2/en
Priority to CA002329509A priority patent/CA2329509C/en
Publication of WO1999061597A2 publication Critical patent/WO1999061597A2/en
Publication of WO1999061597A9 publication Critical patent/WO1999061597A9/en
Publication of WO1999061597A3 publication Critical patent/WO1999061597A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Disclosed herein are novel methods and materials directed to transforming a host cell and expressing exogenous RNA therein. Specifically disclosed are DNA-launching platforms used to introduce a replicating viral segment attached to an exogenous polynucleotide into a cell, whereby the exogenous polynucleotide is expressed in said cell and confers a detectable trait.
PCT/US1999/011250 1998-05-22 1999-05-21 Expression cassette for transformation comprising a modified viral sequence driven by a suitable promoter WO1999061597A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2000550982A JP3959965B2 (en) 1998-05-22 1999-05-21 Improved methods and materials for transformation
EP99953356A EP1086237A2 (en) 1998-05-22 1999-05-21 Expression cassette for transformation comprising a modified viral sequence driven by a suitable promoter
BR9911065-2A BR9911065A (en) 1998-05-22 1999-05-21 Improved processes and materials for transformation
AU43101/99A AU763096B2 (en) 1998-05-22 1999-05-21 Improved methods and materials for transformation
CA002329509A CA2329509C (en) 1998-05-22 1999-05-21 Improved methods and materials for transformation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8652698P 1998-05-22 1998-05-22
US60/086,526 1998-05-22

Publications (3)

Publication Number Publication Date
WO1999061597A2 WO1999061597A2 (en) 1999-12-02
WO1999061597A9 WO1999061597A9 (en) 2000-02-24
WO1999061597A3 true WO1999061597A3 (en) 2000-03-30

Family

ID=22199163

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/011250 WO1999061597A2 (en) 1998-05-22 1999-05-21 Expression cassette for transformation comprising a modified viral sequence driven by a suitable promoter

Country Status (7)

Country Link
EP (1) EP1086237A2 (en)
JP (1) JP3959965B2 (en)
AR (1) AR020324A1 (en)
AU (1) AU763096B2 (en)
BR (1) BR9911065A (en)
CA (1) CA2329509C (en)
WO (1) WO1999061597A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0007231D0 (en) 2000-03-24 2000-05-17 Chiron Spa Modified rna for gene delivery
US6800748B2 (en) * 2001-01-25 2004-10-05 Large Scale Biology Corporation Cytoplasmic inhibition of gene expression and expression of a foreign protein in a monocot plant by a plant viral vector
EP1594956A4 (en) 2003-02-03 2007-08-01 Fraunhofer Usa Inc SYSTEM FOR GENE EXPRESSION IN PLANTS
AU2004278624B2 (en) 2003-10-01 2008-03-20 Japan Science And Technology Agency DNA fragment, method for producing transformant for protein production and utilization thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0194809A1 (en) * 1985-03-07 1986-09-17 Lubrizol Genetics Inc. RNA transformation vector
WO1990012107A1 (en) * 1989-03-31 1990-10-18 The Salk Institute Biotechnology/Industrial Associates, Inc. Recombinant expression system based on satellite tobacco mosaic virus
AU7195191A (en) * 1990-09-07 1992-03-12 Nihon Nohyaku Co., Ltd. Process for production of exogenous gene or its product in plant cells
WO1995007994A2 (en) * 1993-09-15 1995-03-23 Viagene, Inc. Recombinant alphavirus vectors
WO1998036083A1 (en) * 1997-02-14 1998-08-20 Plant Bioscience Limited Methods and means for gene silencing in transgenic plants

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0194809A1 (en) * 1985-03-07 1986-09-17 Lubrizol Genetics Inc. RNA transformation vector
WO1990012107A1 (en) * 1989-03-31 1990-10-18 The Salk Institute Biotechnology/Industrial Associates, Inc. Recombinant expression system based on satellite tobacco mosaic virus
AU7195191A (en) * 1990-09-07 1992-03-12 Nihon Nohyaku Co., Ltd. Process for production of exogenous gene or its product in plant cells
WO1995007994A2 (en) * 1993-09-15 1995-03-23 Viagene, Inc. Recombinant alphavirus vectors
WO1998036083A1 (en) * 1997-02-14 1998-08-20 Plant Bioscience Limited Methods and means for gene silencing in transgenic plants

Also Published As

Publication number Publication date
WO1999061597A2 (en) 1999-12-02
BR9911065A (en) 2001-02-06
WO1999061597A9 (en) 2000-02-24
AU4310199A (en) 1999-12-13
AR020324A1 (en) 2002-05-08
EP1086237A2 (en) 2001-03-28
AU763096B2 (en) 2003-07-10
JP2002516089A (en) 2002-06-04
CA2329509A1 (en) 1999-12-02
CA2329509C (en) 2009-10-06
JP3959965B2 (en) 2007-08-15

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