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USH2071H1 - Streptococcus pneumoniae gene HI0454 - Google Patents

Streptococcus pneumoniae gene HI0454 Download PDF

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Publication number
USH2071H1
USH2071H1 US08/987,147 US98714797A USH2071H US H2071 H1 USH2071 H1 US H2071H1 US 98714797 A US98714797 A US 98714797A US H2071 H USH2071 H US H2071H
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Prior art keywords
nucleic acid
seq
protein
acid fragment
host cell
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Robert Brown Peery
Paul Luther Skatrud
Michele Louise Young Bellido
Patti Jean Treadway
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Eli Lilly and Co
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Eli Lilly and Co
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Assigned to ELI LILLY AND COMPANY reassignment ELI LILLY AND COMPANY ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: BELLIDO, MICHELE LOUISE YOUNG, PEERY, ROBERT BROWN, SKATRUD, PAUL LUTHER, TREADWAY, PATTI JEAN
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56944—Streptococcus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3156—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci from Streptococcus pneumoniae (Pneumococcus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01018—Exo-alpha-sialidase (3.2.1.18), i.e. trans-sialidase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/315—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Streptococcus (G), e.g. Enterococci
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales

Definitions

  • This invention provides isolated DNA sequences, proteins encoded thereby, and methods of using said DNA and protein in a variety of applications.
  • Penicillin resistance in Streptococcus pneumoniae has been particularly problematic. This organism causes upper respiratory tract infections. Modification of a penicillin-binding protein (PBP) underlies resistance to penicillin in the majority of cases.
  • PBP penicillin-binding protein
  • Streptococcus pneumoniae While researchers continue to develop antibiotics effective against a number of microorganisms, Streptococcus pneumoniae has been more refractory. In part, this is because Streptococcus pneumoniae is highly recombinogenic and readily takes up exogenous DNA from its surroundings. Thus, there is a need for new antibacterial compounds and new targets for antibacterial therapy in Streptococcus pneumoniae.
  • the present invention relates to an isolated gene and encoded protein from S. pneumoniae .
  • the invention enables: (1) preparation of probes and primers for use in hybridizations and PCR amplifications, (2) production of proteins and RNAs encoded by said gene and related nucleic acids, and (3) methods to identify compounds that bind and/or inhibit said protein(s).
  • the present invention relates to an isolated nucleic acid molecule encoding a protein designated 454.
  • the invention relates to a nucleic acid molecule comprising the nucleotide sequence identified as SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:4.
  • the present invention relates to a nucleic acid that encodes SEQ ID NO:2.
  • the present invention relates to an isolated protein molecule, wherein said protein molecule comprises the sequence identified as SEQ ID NO:2.
  • the present invention relates to a recombinant DNA vector that incorporates the 454 gene in operable linkage to gene expression sequences enabling the gene to be transcribed and translated in a host cell.
  • the present invention relates to host cells that have been transformed or transfected with the cloned 454 gene such that said gene is expressed in the host cell.
  • This invention also provides a method of determining whether a nucleic acid sequence of the present invention, or fragment thereof, is present in a sample, comprising contacting the sample, under suitable hybridization conditions, with a nucleic acid probe of the present invention.
  • the present invention relates to a method for identifying compounds that bind and/or inhibit the 454 protein.
  • ORF (i.e. “open reading frame”) designates a region of genomic DNA beginning with a Met or other initiation codon and terminating with a translation stop codon, that potentially encodes a protein product.
  • Partial ORF means a portion of an ORF as disclosed herein such that the initiation codon, the stop codon, or both are not disclosed.
  • Consensus sequence refers to an amino acid or nucleotide sequence that may suggest the biological function of a protein, DNA, or RNA molecule. Consensus sequences are identified by comparing proteins, RNAs, and gene homologues from different species.
  • cleavage or “restriction” of DNA refers to the catalytic cleavage of the DNA with a restriction enzyme that acts only at certain sequences in the DNA (viz. sequence-specific endonucleases).
  • restriction enzymes used herein are commercially available and their reaction conditions, cofactors, and other requirements are used in the manner well known to one of ordinary skill in the art. Appropriate buffers and substrate amounts for particular restriction enzymes are specified by the manufacturer or can readily be found in the literature.
  • Essential genes or “essential ORFs” or “essential proteins” refer to genomic information or the protein(s) or RNAs encoded thereby, that when disrupted by knockout mutation, or by other mutation, result in a loss of viability of cells harboring said mutation.
  • Non-essential genes or “non-essential ORFs” or “non-essential proteins” refer to genomic information or the protein(s) or RNAs encoded therefrom which when disrupted by knockout mutation, or other mutation, do not result in a loss of viability of cells harboring said mutation.
  • Minimal gene set refers to a genus comprising about 256 genes conserved among different bacteria such as M. genitalium and H. influenzae .
  • the minimal gene set may be necessary and sufficient to sustain life. See e.g. A. Mushegian and E. Koonin, “A minimal gene set for cellular life derived by comparison of complete bacterial genomes” Proc. Nat. Acad. Sci. 93, 10268-273 (1996).
  • “Knockout mutant” or “knockout mutation” as used herein refers to an in vitro engineered disruption of a region of native chromosomal DNA, typically within a protein coding region, such that a foreign piece of DNA is inserted within the native sequence.
  • a knockout mutation occurring in a protein coding region prevents expression of the wild-type protein. This usually leads to loss of the function provided by the protein.
  • a “knockout cassette” refers to a fragment. of native chromosomal DNA having cloned therein a foreign piece of DNA that may provide a selectable marker.
  • plasmid refers to an extrachromosomal genetic element.
  • the starting plasmids herein are either commercially available, publicly available on an unrestricted basis, or can be constructed from available plasmids in accordance with published procedures.
  • equivalent plasmids to those described are known in the art and will be apparent to the ordinarily skilled artisan.
  • Recombinant DNA cloning vector refers to any autonomously replicating agent, including, but not limited to, plasmids and phages, comprising a DNA molecule to which one or more additional DNA segments can or have been added.
  • recombinant DNA expression vector refers to any recombinant DNA cloning vector, for example a plasmid or phage, in which a promoter and other regulatory elements are present to enable transcription of the inserted DNA.
  • vector refers to a nucleic acid compound used for introducing exogenous DNA into host cells.
  • a vector comprises a nucleotide sequence which may encode one or more protein molecules. Plasmids, cosmids, viruses, and bacteriophages, in the natural state or which have undergone recombinant engineering, are examples of commonly used vectors.
  • complementary refers to the capacity of purine and pyrimidine nucleotides to associate through hydrogen bonding to form double stranded nucleic acid molecules.
  • the following base pairs are related by complementarity: guanine and cytosine; adenine and thymine; and adenine and uracil.
  • complementary applies to all base pairs comprising two single-stranded nucleic acid molecules. “Partially complementary” means one of two single-stranded nucleic acid molecules is shorter than the other, such that one of the molecules remains partially single-stranded.
  • Oligonucleotide refers to a short nucleotide chain comprising from about 2 to about 25 nucleotides.
  • isolated nucleic acid compound refers to any RNA or DNA sequence, however constructed or synthesized, which is locationally distinct from its natural location.
  • a “primer” is a nucleic acid fragment which functions as an initiating substrate for enzymatic or synthetic elongation of, for example, a nucleic acid molecule.
  • promoter refers to a DNA sequence which directs transcription of DNA to RNA.
  • a “probe” as used herein is a labeled nucleic acid compound which can be used to hybridize with another nucleic acid compound.
  • hybridization or “hybridize” as used herein refers to the process by which a single-stranded nucleic acid molecule joins with a complementary strand through nucleotide base pairing.
  • substantially purified means a specific isolated nucleic acid or protein, or fragment thereof, in which substantially all contaminants (i.e. substances that differ from said specific molecule) have been separated from said nucleic acid or protein.
  • a protein may, but not necessarily, be “substantially purified” by the IMAC method as described herein.
  • “Selective hybridization” refers to hybridization under conditions of high stringency.
  • the degree of hybridization between nucleic acid molecules depends upon, for example, the degree of complementarity, the stringency of hybridization, and the length of hybridizing strands.
  • stringency relates to nucleic acid hybridization conditions. High stringency conditions disfavor non-homologous base pairing. Low stringency conditions have the opposite effect. Stringency may be altered, for example, by changes in temperature and salt concentration. Typical high stringency conditions comprise hybridizing at 50° C. to 65° C. in 5 ⁇ SSPE and 50% formamide, and washing at 50° C. to 65° C. in 0.5 ⁇ SSPE; typical low stringency conditions comprise hybridizing at 35° C. to 37° C. in 5 ⁇ SSPE and 40% to 45% formamide and washing at 42° C. in 1 ⁇ -2 ⁇ SSPE.
  • SSPE denotes a hybridization and wash solution comprising sodium chloride, sodium phosphate, and EDTA, at pH 7.4.
  • a 20 ⁇ solution of SSPE is made by dissolving 174 g of NaCl, 27.6 g of NaH 2 PO4. H 2 O, and 7.4 g of EDTA in 800 ml of H 2 O. The pH is adjusted with NaOH and the volume brought to 1 liter.
  • SSC denotes a hybridization and wash solution comprising sodium chloride and sodium citrate at pH 7.
  • a 20 ⁇ solution of SSC is made by dissolving 175 g of NaCl and 88 g of sodium citrate in 800 ml of H 2 O. The volume is brought to 1 liter after adjusting the pH with 10N NaOH.
  • the 454 gene disclosed herein (SEQ ID NO:1) and related nucleic acids (viz. SEQ ID NO:3 and SEQ ID NO:4) encode an essential protein that is a member of the minimal gene set.
  • the proteins of this invention are purified, and used in a screen to identify compounds that bind and/or inhibit the activity of said proteins.
  • a variety of suitable screens are contemplated for this purpose.
  • the protein(s) can be labeled by known techniques, such as radiolabeling or fluorescent tagging, or by labeling with biotin/avidin. Thereafter, binding of a test compound to a labeled protein can be determined by any suitable means, well known to the skilled artisan.
  • DNA molecules of this invention can be generated by general cloning methods. PCR amplification using oligonucleotide primers targeted to any suitable region of SEQ ID NO:1 is preferred. Methods for PCR amplification are widely known in the art. See e.g. PCR Protocols: A Guide to Method and Application , Ed. M. Innis et al., Academic Press (1990) or U.S. Pat. No. 4,889,818, which hereby is incorporated by reference.
  • a PCR comprises DNA, suitable enzymes, primers, and buffers, and is conveniently carried out in a DNA Thermal Cycler (Perkin Elmer Cetus, Norwalk, Conn.). A positive PCR result is determined by, for example, detecting an appropriately-sized DNA fragment following agarose gel electrophoresis.
  • the DNAs of the present invention may also be produced using synthetic methods well known in the art. (See, e.g., E. L. Brown, R. Belagaje, M. J. Ryan, and H. G. Khorana, Methods in Enzymology , 68:109-151 (1979)).
  • An apparatus such as the Applied Biosystems Model 380A or 380B DNA synthesizers (Applied Biosystems, Inc., 850 Lincoln Center Drive, Foster City, Calif. 94404) may be used to synthesize DNA.
  • Synthetic methods rely upon phosphotriester chemistry [See, e.g., M. J. Gait, ed., Oligonucleotide Synthesis, A Practical Approach , (1984)], or phosphoramidite chemistry.
  • the present invention relates further to substantially purified proteins encoded by the gene disclosed herein.
  • proteins can be synthesized by different methods, for example, chemical methods or recombinant methods, as described in U.S. Pat. No. 4,617,149, which hereby is incorporated by reference.
  • polypeptides may be synthesized by solid-phase methodology utilizing an Applied Biosystems 430A peptide synthesizer (Applied Biosystems, Foster City, Calif.) and synthesis cycles supplied by Applied Biosystems.
  • Protected amino acids such as t-butoxycarbonyl-protected amino acids, and other reagents are commercially available from many chemical supply houses.
  • the proteins of the present invention can also be made by recombinant DNA methods. Recombinant methods are preferred if a high yield is desired. Recombinant methods involve expressing the cloned gene in a suitable host cell. The gene is introduced into the host cell by any suitable means, well known to those skilled in the art. While chromosomal integration of the cloned gene is within the scope of the present invention, it is preferred that the cloned gene be maintained extra-chromosomally, as part of a vector in which the gene is in operable-linkage to a promoter.
  • Recombinant methods can also be used to overproduce a membrane-bound or membrane-associated protein.
  • membranes prepared from recombinant cells expressing such proteins provide an enriched source of the protein.
  • Procaryotes are generally used for cloning DNA sequences and for constructing vectors.
  • Escherichia coli K12 strain 294 ATCC No. 31446
  • Other strains of E. coli , bacilli such as Bacillus subtilis , enterobacteriaceae such as Salmonella typhimurium or Serratia marcescans , various Pseudomonas species may also be employed as host cells in cloning and expressing the recombinant proteins of this invention.
  • various strains of Streptococcus and Streptocmyces are also be employed as host cells in cloning and expressing the recombinant proteins of this invention.
  • a gene For effective recombinant protein production, a gene must be linked to a promoter sequence.
  • Suitable bacterial promoters include b-lactamase [e.g. vector pGX2907, ATCC 39344, contains a replicon and b-lactamase gene], lactose systems [Chang et al., Nature (London), 275:615 (1978); Goeddel et al., Nature (London), 281:544 (1979)], alkaline phosphatase, and the tryptophan (trp) promoter system [vector pATH1 (ATCC 37695)] designed for the expression of a trpE fusion protein.
  • b-lactamase e.g. vector pGX2907, ATCC 39344, contains a replicon and b-lactamase gene
  • lactose systems [Chang et al., Nature (London), 275:615 (1978);
  • Hybrid promoters such as the tac promoter (isolatable from plasmid pDR540, ATCC-37282) are also suitable. Promoters for use in bacterial systems also will contain a Shine-Dalgarno sequence, operably linked to the DNA encoding the desired polypeptides. These examples are illustrative rather than limiting.
  • yeast Saccharomyces cerevisiae is commonly used.
  • Other yeasts, such as Kluyveromyces lactis are also suitable.
  • the plasmid YRp7 (ATCC-40053), for example, may be used. See, e.g., L. Stinchcomb, et al., Nature , 282:39 (1979); J. Kingsman et al., Gene , 7:141 (1979); S. Tschemper et al., Gene , 10:157 (1980).
  • Plasmid YRp7 contains the TRP1 gene, a selectable marker for a trp1 mutant.
  • An expression vector carrying a nucleic acid or gene of the present invention is transformed or transfected into a suitable host cell using standard methods.
  • Cells that contain the vector are propagated under conditions suitable for expression of a recombinant protein.
  • suitable growth conditions would incorporate the appropriate inducer.
  • the recombinantly-produced protein may be purified from cellular extracts of transformed cells by any suitable means.
  • a gene is modified at the 5′ end, or at some other position, such that the encoded protein incorporates several histidine residues (viz. “histidine tag”).
  • This “histidine tag” enables “immobilized metal ion affinity chromatography” (IMAC), a single-step protein purification method described in U.S. Pat. No. 4,569,794, which hereby is incorporated by reference.
  • IMAC immobilized metal ion affinity chromatography
  • the proteins of the invention can be encoded by a large genus of different nucleic acid sequences. This invention further comprises said genus.
  • ribonucleic acid compounds of the invention may be prepared using the polynucleotide synthetic methods discussed supra, or they may be prepared enzymatically using RNA polymerase to transcribe a DNA template.
  • RNA polymerase from the bacteriophage T7 or the bacteriophage SP6. These RNA polymerases are highly specific, requiring the insertion of bacteriophage-specific sequences at the 5′ end of a template. See, J. Sambrook, et al., supra, at 18.82-18.84.
  • This invention also provides nucleic acids that are complementary to the sequences disclosed herein.
  • the present invention also provides probes and primers, useful for a variety of molecular biology techniques including, for example, hybridization screens of genomic or subgenomic libraries, or detection and quantification of mRNA species as a means to analyze gene expression.
  • a nucleic acid compound is provided comprising any of the sequences disclosed herein, or a complementary sequence thereof, or a fragment thereof, which is at least 15 base pairs in length, and which will hybridize selectively to Streptococcus pneumoniae DNA or mRNA.
  • the 15 or more base pair compound is DNA.
  • a probe or primer length of at least 15 base pairs is dictated by theoretical and practical considerations. See e.g. B. Wallace and G. Miyada, “Oligonucleotide Probes for the Screening of Recombinant DNA Libraries,” In Methods in Enzymology , Vol. 152, 432-442, Academic Press (1987).
  • probes and primers of this invention can be prepared by methods well known to those skilled in the art (See e.g. Sambrook et al. supra). In a preferred embodiment the probes and primers are synthesized by the polymerase chain reaction (PCR).
  • PCR polymerase chain reaction
  • the present invention also relates to recombinant DNA cloning vectors and expression vectors comprising the nucleic acids of the present invention.
  • Preferred nucleic acid vectors are those that comprise DNA.
  • the skilled artisan understands that choosing the most appropriate cloning vector or expression vector depends on the availability of restriction sites, the type of host cell into which the vector is to be transfected or transformed, the purpose of transfection or transformation (e.g., stable transformation as an extrachromosomal element, or integration into a host chromosome), the presence or absence of readily assayable or selectable markers (e.g., antibiotic resistance and metabolic markers of one type and another), and the number of gene copies desired in the host cell.
  • readily assayable or selectable markers e.g., antibiotic resistance and metabolic markers of one type and another
  • Suitable vectors comprise RNA viruses, DNA viruses, lytic bacteriophages, lysogenic bacteriophages, stable bacteriophages, plasmids, viroids, and the like.
  • the most preferred vectors are plasmids.
  • Host cells harboring the nucleic acids disclosed herein are also provided by the present invention.
  • a preferred host is E. coli transfected or transformed with a vector comprising a nucleic acid of the present invention.
  • the invention also provides a host cell capable of expressing a gene described herein, said method comprising transforming or otherwise introducing into a host cell a recombinant DNA vector comprising an isolated DNA sequence that encodes said gene.
  • the preferred host cell is any strain of E. coli that can accommodate high level expression of an exogenously introduced gene.
  • Transformed host cells are cultured under conditions well known to skilled artisans, such that said gene is expressed, thereby producing the encoded protein in the recombinant host cell.
  • a method for identifying such compounds comprises contacting a suitable protein or membrane preparation with a test compound and monitoring by any suitable means an interaction and/or inhibition of a protein of this invention.
  • the instant invention provides a screen for compounds that interact with the proteins of the invention, said screen comprising:
  • the screening method of this invention may be adapted to automated procedures such as a PANDEX® (Baxter-Dade Diagnostics) system, allowing for efficient high-volume screening of compounds.
  • PANDEX® Boxter-Dade Diagnostics
  • a protein is prepared as described herein, preferably using recombinant DNA technology.
  • a test compound is introduced into a reaction vessel containing said protein.
  • the reaction/interaction of said protein and said compound is monitored by any suitable means.
  • a radioactively-labeled or chemically-labeled compound or protein is used.
  • a specific association between the test compound and protein is monitored by any suitable means.
  • binding of 454 by a test compound is determined by any suitable means. For example, in one method radioactively-labeled or chemically-labeled test compound may be used. Binding of the protein by the compound is assessed, for example, by quantifying bound label versus unbound label using any suitable method. Binding of a test compound may also be carried out by a method disclosed in U.S. Pat. No. 5,585,277, which hereby is incorporated by reference.
  • binding of a test compound to a protein is assessed by monitoring the ratio of folded protein to unfolded protein, for example by monitoring sensitivity of said protein to a protease, or amenability to binding of said protein by a specific antibody against the folded state of the protein.
  • a ligand that binds 454, or related fragment thereof is identified, for example, by combining a test ligand with 454 under conditions that cause the protein to exist in a ratio of folded to unfolded states. If the test ligand binds the folded state of the protein, the relative amount of folded protein will be higher than in the case of a test ligand that does not bind the protein.
  • the ratio of protein in the folded versus unfolded state is easily determinable by, for example, susceptibility to digestion by a protease, or binding to a specific antibody, or binding to chaperonin protein, or binding to any suitable surface.
  • An expression vector suitable for expressing S. pneumoniae 454 in a variety of procaryotic host cells, such as E. coli , is easily made.
  • the vector contains an origin of replication (Ori), an ampicillin resistance gene (Amp) useful for selecting cells which have incorporated the vector following a tranformation procedure, and further comprises the T7 promoter and T7 terminator sequences in operable linkage to the coding region.
  • Plasmid pET11A (obtained from Novogen, Madison, Wis.) is a suitable parent plasmid. pET11A is linearized by restriction with endonucleases NdeI and BamHI.
  • Linearized pET11A is ligated to a DNA fragment bearing NdeI and BamHI sticky ends and comprising the coding region of the S. pneumoniae 454 gene (SEQ ID NO:1).
  • the coding region for 454 is easily produced by PCR technology using suitably designed primers to the ends of the coding region specified in SEQ ID NO:1.
  • the 454 gene used in this construction is slightly modified at the 5′ end (amino terminus of encoded protein) in order to simplify purification of the encoded protein product.
  • an oligonucleotide encoding 8 histidine residues is inserted after the ATG start codon. Placement of the histidine residues at the amino terminus of the encoded protein serves to enable the IMAC one-step protein purification procedure.
  • An expression vector that carries the 454 Gene from the S. pneumoniae genome as disclosed herein and which is operably-linked to an expression promoter is transformed into E. coli BL21 (DE3) (hsdS gal lcIts857 ind1Sam7nin5lacUV5-T7gene 1) using standard methods (see Example 4). Transformants, selected for resistance to ampicillin, are chosen at random and tested for the presence of the vector by agarose gel electrophoresis using quick plasmid preparations. Colonies which contain the vector are grown in L broth and the protein product encoded by the vector-borne ORF is purified by immobilized metal ion affinity chromatography (IMAC), essentially as described in U.S. Pat. No. 4,569,794.
  • IMAC immobilized metal ion affinity chromatography
  • the IMAC column is prepared as follows.
  • a metal-free chelating resin e.g. Sepharose 6B IDA, Pharmacia
  • a suitable metal ion e.g. Ni(II), Co(II), or Cu(II)] by adding a 50 mM metal chloride or metal sulfate aqueous solution until about 75% of the interstitial spaces of the resin are saturated with colored metal ion.
  • the column is then ready to receive a crude cellular extract containing the recombinant protein product.
  • the bound protein is eluted in any suitable buffer at pH 4.3, or preferably with an imidizole-containing buffer at pH 7.5.

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US08/987,147 1996-12-13 1997-12-08 Streptococcus pneumoniae gene HI0454 Abandoned USH2071H1 (en)

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US08/987,147 USH2071H1 (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene HI0454

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US3628196P 1996-12-13 1996-12-13
US08/987,147 USH2071H1 (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene HI0454

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US08/986,765 Expired - Fee Related US6071724A (en) 1996-12-13 1997-12-08 Streptococcus pneumoniaegene sequence era
US08/987,151 Expired - Fee Related US6162617A (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence dnaG
US08/986,963 Expired - Fee Related US5958730A (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence FtsY
US08/987,146 Expired - Fee Related US6350866B1 (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence FtsZ
US08/987,152 Expired - Fee Related US5981281A (en) 1996-12-13 1997-12-08 Method for knockout mutagenesis in Streptococcus pneumoniae
US08/987,119 Abandoned USH2070H1 (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence for DNA ligase
US08/986,967 Abandoned USH2023H1 (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae sequence GrpE
US08/987,147 Abandoned USH2071H1 (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene HI0454
US08/986,769 Expired - Fee Related US6074847A (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence HI1146
US08/987,144 Expired - Fee Related US6060282A (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence Dpj
US08/987,122 Expired - Fee Related US5910580A (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence HI1648
US08/987,121 Expired - Fee Related US6268175B1 (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence GCP
US08/986,768 Expired - Fee Related US6271000B1 (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence mraY
US08/987,123 Expired - Fee Related US6136557A (en) 1996-12-13 1997-12-08 Strepococcus pneumoniae gene sequence FtsH
US09/198,284 Abandoned USH2019H1 (en) 1996-12-13 1998-11-23 Streptococcus pneumoniae gene sequence HI1648

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US08/986,765 Expired - Fee Related US6071724A (en) 1996-12-13 1997-12-08 Streptococcus pneumoniaegene sequence era
US08/987,151 Expired - Fee Related US6162617A (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence dnaG
US08/986,963 Expired - Fee Related US5958730A (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence FtsY
US08/987,146 Expired - Fee Related US6350866B1 (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence FtsZ
US08/987,152 Expired - Fee Related US5981281A (en) 1996-12-13 1997-12-08 Method for knockout mutagenesis in Streptococcus pneumoniae
US08/987,119 Abandoned USH2070H1 (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence for DNA ligase
US08/986,967 Abandoned USH2023H1 (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae sequence GrpE

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US08/986,769 Expired - Fee Related US6074847A (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence HI1146
US08/987,144 Expired - Fee Related US6060282A (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence Dpj
US08/987,122 Expired - Fee Related US5910580A (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence HI1648
US08/987,121 Expired - Fee Related US6268175B1 (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence GCP
US08/986,768 Expired - Fee Related US6271000B1 (en) 1996-12-13 1997-12-08 Streptococcus pneumoniae gene sequence mraY
US08/987,123 Expired - Fee Related US6136557A (en) 1996-12-13 1997-12-08 Strepococcus pneumoniae gene sequence FtsH
US09/198,284 Abandoned USH2019H1 (en) 1996-12-13 1998-11-23 Streptococcus pneumoniae gene sequence HI1648

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US (15) US6071724A (fr)
EP (1) EP0950108A1 (fr)
AU (1) AU5793798A (fr)
CA (1) CA2274311A1 (fr)
WO (1) WO1998026072A1 (fr)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0885297A4 (fr) * 1996-02-20 1999-11-24 Smithkline Beecham Corp Nouveaux polypeptides era
US6165989A (en) * 1996-05-14 2000-12-26 Smithkline Beecham Corporation Era of Streptococcus pneumoniae
US5681694A (en) * 1996-06-18 1997-10-28 Eli Lilly And Company Murd protein method and kit for identification of inhibitors
US5910414A (en) 1996-10-15 1999-06-08 Smithkline Beecham Corporation Topoisomerase I of streptococcus pneumoniae
US5789202A (en) * 1996-10-17 1998-08-04 Eli Lilly And Company DNA encoding a novel penicillin binding protein from streptococcus pneumoniae
US6096518A (en) * 1996-10-24 2000-08-01 Smithkline Beecham Corporation DNA encoding SPO/REL polypeptides of streptococcus
DE69739981D1 (de) 1996-10-31 2010-10-14 Human Genome Sciences Inc Streptococcus pneumoniae-Antigene und Impfstoffe
US5821335A (en) * 1996-11-19 1998-10-13 Eli Lilly And Company Biosynthetic gene murg from streptococcus pneumoniae
US6287803B1 (en) * 1996-11-27 2001-09-11 Smithkline Beecham Corporation Polynucleotides encoding a novel era polypeptide
US5948645A (en) * 1996-12-04 1999-09-07 Eli Lilly And Company Biosynthetic gene muri from Streptococcus pneumoniae
US6800744B1 (en) 1997-07-02 2004-10-05 Genome Therapeutics Corporation Nucleic acid and amino acid sequences relating to Streptococcus pneumoniae for diagnostics and therapeutics
US7081530B1 (en) 1997-07-02 2006-07-25 sanofi pasteur limitée Nucleic acid and amino acid sequences relating to Streptococcus pneumoniae for diagnostics and therapeutics
US5840538A (en) * 1997-08-06 1998-11-24 Smithkline Beecham Corporation Lgt
EP0896061A3 (fr) * 1997-08-08 2000-07-26 Smithkline Beecham Corporation Gène rpoA de Staphylococcus aureus
EP0896060A3 (fr) * 1997-08-08 2000-07-19 Smithkline Beecham Corporation Gène rpoA de Staphylococcus aureus
US5929045A (en) 1997-08-12 1999-07-27 Smithkline Beecham Corporation Recombinant expression of polynucleotides encoding the UDP-N-acetylmuramoylalanine:D-glutamate ligase (MurD) of Streptococcus pneumoniae
US6197300B1 (en) * 1997-08-12 2001-03-06 Smithkline Beecham Corporation ftsZ
US5888770A (en) * 1997-08-26 1999-03-30 Smithkline Beecham Corporation Spoiiie
US6072032A (en) * 1997-08-29 2000-06-06 Smithkline Beecham Corporation FtsY polypeptides from Streptococcus pneumoniae
EP0915161A3 (fr) * 1997-10-21 1999-08-25 Smithkline Beecham Corporation DnaG DNA primase de Streptococcus pneumoniae
GB9726586D0 (en) * 1997-12-16 1998-02-11 Microscience Ltd Protein and compositions containing it
CA2315252A1 (fr) * 1997-12-31 1999-07-08 Millennium Pharmaceuticals, Inc. Genes bacteriens essentiels et leur utilisation
GB9808423D0 (en) * 1998-04-22 1998-06-17 Glaxo Group Ltd Bacterial polypeptide family
US6274719B1 (en) * 1998-04-24 2001-08-14 Smithkline Beecham Corporation Gcp
EP1087984A4 (fr) * 1998-05-28 2005-02-09 Smithkline Beecham Corp acpS
US6190881B1 (en) * 1998-06-05 2001-02-20 Smithkline Beecham Corporation Ribonucleotide diphosphate reductase, nrdF, of streptococcus pneumoniae
EP1095133A4 (fr) * 1998-07-02 2003-06-11 Smithkline Beecham Corp Proteines multimeres de ftsz et leurs applications
US6936252B2 (en) 1998-07-27 2005-08-30 Microbial Technics Limited Streptococcus pneumoniae proteins and nucleic acid molecules
EP1144640A3 (fr) * 1998-07-27 2001-11-28 Microbial Technics Limited Acides nucleiques et proteines de streptococcus pneumoniae
EP1790729A3 (fr) * 1998-07-27 2007-07-25 Sanofi Pasteur Limited Protéines de Streptococcus pneumoniae et molécules d'acide nucléique
US20030134407A1 (en) 1998-07-27 2003-07-17 Le Page Richard William Falla Nucleic acids and proteins from Streptococcus pneumoniae
US6515119B1 (en) * 1998-09-30 2003-02-04 Millennium Pharmaceuticals, Inc. Use of S-ydcB and B-ydcB, essential bacterial genes
US6537774B1 (en) 1998-10-14 2003-03-25 Smithkline Beecham Corporation UPS (undecaprenyl diphosphate synthase
US6110685A (en) * 1998-10-28 2000-08-29 Smithkline Beecham Corporation infB
WO2000025818A1 (fr) * 1998-11-04 2000-05-11 Smithkline Beecham Corporation POLYPEPTIDES ET POLYNUCLEOTIDES DE LA FAMILLE asuE/trmU
US6346395B1 (en) * 1999-01-27 2002-02-12 Smithkline Beecham Corp. Nucleic acids encoding Streptococcus pneumoniae FabG
US6110704A (en) 1999-01-28 2000-08-29 Smithkline Beecham Corporation 3-ketoacyl-ACP-reductase (FabG) of Staphylococcus aureus
US6346396B1 (en) * 1999-01-29 2002-02-12 Jianzhong Huang MurA
CA2371596A1 (fr) 1999-04-30 2000-11-09 Hybrigenics S.A. Collection d'adn procaryote d'helicobacter pylori permettant l'identification des interactions proteine-proteine utilisable pour deux systemes hybrides et applications
WO2001007458A1 (fr) * 1999-07-22 2001-02-01 Smithkline Beecham Corporation POLYPEPTIDES lacR
US6168797B1 (en) * 1999-08-18 2001-01-02 Smithkline Beecham Corporation FabF
US6951729B1 (en) 1999-10-27 2005-10-04 Affinium Pharmaceuticals, Inc. High throughput screening method for biological agents affecting fatty acid biosynthesis
US6613553B1 (en) * 2000-02-04 2003-09-02 St. Jude Children's Research Hospital Enoyl reductases and methods of use thereof
GB2364054B (en) * 2000-03-24 2002-05-29 Smithkline Beecham Corp Method of amplifying quinolone-resistance-determining-regions and identifying polymorphic variants thereof
US7056697B2 (en) 2000-10-06 2006-06-06 Affinium Pharmaceuticals, Inc. FabK variant
US6821746B2 (en) 2000-10-06 2004-11-23 Affinium Pharmaceuticals, Inc. Methods of screening for FabK antagonists and agonists
US7048926B2 (en) 2000-10-06 2006-05-23 Affinium Pharmaceuticals, Inc. Methods of agonizing and antagonizing FabK
CA2424987A1 (fr) * 2000-10-06 2002-04-18 Smithkline Beecham Corporation Procedes d'utilisation d'agonistes et d'antagonistesdes polypeptides fabk
US7033795B2 (en) 2000-10-06 2006-04-25 Affinium Pharmaceuticals, Inc. FabK variant
AU2002226212B2 (en) * 2000-12-19 2006-11-16 Targanta Therapeutics Inc. Compositions and methods involving staphylococcus aureus protein STAAU-R9
CN1643163A (zh) * 2002-02-20 2005-07-20 Ncc技术投资私人有限公司 关于癌症诊断的材料和方法
EP1615950A2 (fr) * 2003-04-15 2006-01-18 Intercell AG Antigenes s-pneumoniae
GB0318688D0 (en) * 2003-08-08 2003-09-10 Chiron Srl Streptococcus pneumoniae knockout mutants
US20070149496A1 (en) * 2003-10-31 2007-06-28 Jack Tuszynski Water-soluble compound
WO2013043643A1 (fr) * 2011-09-21 2013-03-28 St. Jude Children's Research Hospital Souche vivante atténuée de streptococcus pneumoniae et vaccin pour la protection contre une maladie pneumococcique
WO2019025958A1 (fr) * 2017-07-31 2019-02-07 Enzibeta Biotech Pvt. Ltd. Séquences géniques modifiées codant pour la choline oxydase et procédé de préparation de la bétaïne l'utilisant
CN119751572A (zh) * 2024-12-24 2025-04-04 昆明理工大学 榆黄菇肽及其制备和应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4762786A (en) * 1984-09-27 1988-08-09 Eli Lilly And Company Vectors and conditions which allow genetic transformation of cephalosporium
US5747281A (en) * 1984-09-28 1998-05-05 Cornell Research Foundation, Inc. System useful for the production of proteins from recombinant DNA in single celled organisms
US4569794A (en) * 1984-12-05 1986-02-11 Eli Lilly And Company Process for purifying proteins and compounds useful in such process
RU1780542C (ru) * 1985-04-22 1992-12-07 Эли Лилли Энд Компани Способ конструировани рекомбинантной плазмидной ДНК pPS 20, кодирующей изопенициллин-N-синтетазу, способ получени штамма СернаLоSроRIUм асRемоNIUм, обладающего активностью изопенициллин-N-синтетазы
US4892819A (en) * 1985-11-25 1990-01-09 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum
US4960707A (en) * 1987-08-17 1990-10-02 Associated Universities, Inc. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae
US5070020A (en) * 1988-05-09 1991-12-03 Eli Lilly And Company Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase
GB9100825D0 (en) * 1991-01-15 1991-02-27 Univ Guelph Pasteurella haemolytica glycoprotease gene and the purified enzyme
IL109410A0 (en) * 1993-04-30 1994-07-31 Ell Lilly & Company Fema gene of staphylococcus epidermidis, fema protein, and vectors and microorganisms comprising the fema gene
US5591839A (en) * 1994-08-18 1997-01-07 Glyko, Inc. Polynucleotides encoding α2-3 neuraminidase
JP3571378B2 (ja) * 1994-09-30 2004-09-29 扶桑薬品工業株式会社 感染症診断用プローブ
US5705352A (en) * 1995-02-27 1998-01-06 Eli Lilly And Company Multiple drug resistance gene of Aspergillus fumigatus
US5773214A (en) * 1995-02-27 1998-06-30 Eli Lilly And Company Multiple drug resistance gene of aspergillus flavus
US5691161A (en) * 1996-08-01 1997-11-25 Eli Lilly And Company Peptidoglycan biosynthetic mura protein from Streptococcus pneumoniae
US6197300B1 (en) * 1997-08-12 2001-03-06 Smithkline Beecham Corporation ftsZ

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Boehanger Mannheim Biochemicals, 1991 Catalog p. 557.* *
Fleischmann, et al. "Whole-Genome Random Sequencing and Assembly of Haemophilus influenzae Rd." Science 269:496-512 (July 28, 1995).
Gibco BRL, Catalogue and Reference Guide 1992, p. 292.* *
Lindler et al. Journal of Bacteriology, Jul. 1987 169(7): 3199-3208.* *
New England Biolabs catalog 1986/87, pp. 60-62.* *
Promega, 1993/94 Catalog, pp. 90-91.* *
Stratagene 1991 Product Catalog, p. 66.* *

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US6060282A (en) 2000-05-09
US6162617A (en) 2000-12-19
USH2023H1 (en) 2002-05-07
US5910580A (en) 1999-06-08
US6074847A (en) 2000-06-13
EP0950108A1 (fr) 1999-10-20
WO1998026072A1 (fr) 1998-06-18
US6350866B1 (en) 2002-02-26
USH2019H1 (en) 2002-04-02
US5981281A (en) 1999-11-09
CA2274311A1 (fr) 1998-06-18
US5958730A (en) 1999-09-28
AU5793798A (en) 1998-07-03
US6271000B1 (en) 2001-08-07
US6071724A (en) 2000-06-06
US6268175B1 (en) 2001-07-31
US6136557A (en) 2000-10-24
USH2070H1 (en) 2003-07-01

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