TWM526071U - Rapid screening processing chip for detecting pesticides residue of vegetables and fruits - Google Patents

Description

Fast screening processor for detecting pesticide residues in fruits and vegetables

This work is about a test strip for detecting pesticide residues, in particular, a fast-screening wafer for detecting pesticide residues in fruits and vegetables for detecting the content of chlorobenzene in fruits and vegetables.

In recent years, food safety problems have emerged in an endless stream. A series of food safety incidents not only make consumers nervous, but also the moral integrity of food industry, and the failure of food safety units to effectively control consumers can only be self-defeating. Therefore, it is also one of the focuses of the public to pay attention to whether the agricultural products (vegetables and fruits) are over-medicated in the process of planting, which leads to excessive pesticide residues in fruits and vegetables.

Previous methods for analyzing the concentration of pesticide residues in fruits and vegetables, such as spectrophotometry, nuclear magnetic resonance spectroscopy, thin layer chromatography, atomic absorption spectroscopy, gas chromatography, liquid chromatography, fluorescence, etc. Among them, gas chromatography and liquid chromatography are commonly used, and they have good reproducibility, sensitivity, and the advantages of pesticide type and concentration. However, such analysis methods need to follow the standard test procedure flow, and carry out sample pre-processing and instrument operation analysis through laboratory professionals with professional knowledge, in order to obtain effective test results, so the above-mentioned analysis methods, general The problem is that it is labor-intensive and time-consuming, and it cannot be quickly and conveniently used for the detection of pesticide residues in a large number of agricultural products. This is not anxious for consumers who are eager to learn more.

In recent years, biochemical reactions and electrochemical techniques have been developed to detect pesticides of the type of enzyme inhibitors, such as U.S. Patent No. 6,406,876, which discloses that the immobilized enzyme technology does not require the preparation of a large amount of test solution, but such immobilized enzymes The disadvantage is that the manufacturing process is complicated. The cost is high and the electrode storage conditions are quite rigorous, so non-professional technicians cannot analyze it.

Secondly, the new patent of Taiwan Patent No. M376764 and the invention patent of the Chinese Patent No. CN101082599, although a simple method of fixing the enzyme on the analysis device is proposed to discriminate the concentration of the pesticide residue, but the design method is also complicated, and it is necessary to separately The enzyme and the related chemical reaction substance are separately stored in the electrode and the sample container, and the two-stage reaction is separately performed, which may cause an error in the analysis test.

The patent case No. I301541 of Taiwan Patent also proposes to immobilize the enzyme on the electrode, and to judge the concentration of the pesticide in the water by the degree of inhibition of the enzyme by the pesticide, but the method of fixing the enzyme is complicated, and it is difficult in mass production and for The quantitative method of pesticide residue concentration is also expressed only by the enzyme inhibition rate, and it is impossible to specifically let the device user know the actual pesticide residue.

In addition, the common enzyme test piece commonly used in the workshop has a main structure in which three electrodes are formed on the test piece, one of which is a working electrode for measuring the main working voltage, and the other is an auxiliary electrode as an electrode for the reduction reaction, It is a reference electrode, which is used as an electrode for detecting current correction. The detection method of the three electrodes can be used to correct the working electrode by the third electrode, that is, the reference electrode. However, the enzyme analysis method refers to a type B. The choline sterol ester chymase method mainly inhibits the reaction of aminol formates and organophosphorus agents in pesticides, that is, the phenomenon of using enzyme reaction as a qualitative screening method for residual pesticides. Therefore, enzyme analysis method At best, only the residual amount of organic phosphorus in fruits and vegetables can be detected, and it is impossible to detect the content of chlorobenzene which is seriously toxic to the environment and the human body. In addition, in order to prevent electrode oxidation, the general enzyme test strip usually coats the outer surface of the electrode with a layer of carbon black to prevent the electrode from being rapidly oxidized by environmental factors. However, the impedance of the carbon black layer is high, which may affect the detection. Accuracy, which is one of the main reasons for the ineffective detection of enzyme test strips.

Furthermore, another method for measuring pesticides in fruits and vegetables is to use carbon electrodes to modify the electrode potential and redox for analysis. Although the carbon electrode measurement method has high sensitivity characteristics, only Carbon electrode measurement is not only low in reproducibility, but also easy to passivate the electrode. More importantly, the carbon electrode is expensive, and the one-time measurement is not only costly, but also cannot be widely used by the public. Not very economical.

The present invention is a fast-screening wafer for detecting pesticide residues in fruits and vegetables, and the fluorinated polymeric derivative in the circuit layer of the wafer is processed by the fast screening so that the reagent is deposited on the circuit layer, and the plasma chemical deposition of Nafion and derivative is utilized. The material is used to modify the wafer electrode, and the electrochemical potential interval and redox are professionally analyzed to detect the structure of the chlorobenzene of the pesticide, and to determine the content of the toxic substance of the chlorobenzene to obtain high sensitivity detection and Excellent reproducibility.

The fast-screening wafer for measuring pesticide residues in fruits and vegetables is covered on the outer surface of the electrode by a silver anti-oxidation layer on the circuit layer. In addition to preventing oxidation, silver has good conductivity and low impedance, so the detection can be relatively improved. Speed and accuracy.

Therefore, the fast-screening wafer for detecting pesticide residues in fruits and vegetables proposed by the present invention comprises a substrate layer, a circuit layer, a fluorinated layer and a barrier layer. The circuit layer is disposed on the base layer and has a first electrode, a second electrode and a sensing pole. The first electrode is connected to the sensing pole, and the second electrode is opposite to the sensing pole. Interval not connected. The fluorinated layer is a fluorinated polymeric derivative overlying the circuit layer. The barrier layer covers the fluorination layer, the circuit layer and the base layer, so that a portion of the first electrode and the second electrode of the circuit layer are exposed, and the barrier layer has a sense corresponding to the circuit layer Measure the detection hole of the pole.

According to the above-mentioned fast screening processing wafer for detecting pesticide residue of fruits and vegetables, wherein the circuit layer is silver, and the portion of the circuit wiring of the fast screening processing chip exposed is silver white.

According to the above-mentioned fast screening processing wafer for detecting pesticide residue of fruits and vegetables, further comprising an anti-oxidation layer covering the circuit layer so as to be sandwiched between the circuit layer and the fluorinated layer, the anti-oxidation layer It is cerium oxide.

The first electrode has a first guiding end at the front end and a first extending end extending from the first guiding end and connected to the sensing pole according to the above-mentioned fast screening processing wafer for detecting pesticide residues in fruits and vegetables a connecting portion, the second electrode has a second guiding end at the front end and a second connecting portion extending outward from the second guiding end, the second connecting portion of the second electrode and the sensing pole There is a gap left between the first connection segment corresponding to the first electrode portion, the sensing pole, the second connecting portion of the second electrode portion, and the gap, so as to make a portion of the gap A connecting segment, the sensing pole, a portion of the second connecting segment, and the gap are all exposed to the outside.

According to the above-mentioned fast screening processing wafer for detecting pesticide residue of fruits and vegetables, wherein the base layer is made of plastic material or paper.

According to the above-mentioned fast screening processing wafer for detecting pesticide residues in fruits and vegetables, wherein the fast screening processing wafer is inserted on a connecting port of a mobile reading device to detect the value of pesticide residues.

Therefore, compared with the existing enzyme test pieces, the operation is complicated, the detection speed is slow, and the impedance is high, resulting in inaccurate detection, and the carbon electrode is expensive, reproducible, and easy to use the electrode passivation problem. The fast-screening wafer of the present invention utilizes a fluorinated polymeric derivative in the circuit layer to modify the wafer electrode, and performs professional analysis by electrochemical potential interval and redox, which can quickly detect the structure of the pesticide chlorobenzene, and Determine the amount of toxic substances in chlorobenzene to achieve high sensitivity detection and excellent reproducibility. In addition, by covering the outer surface of the electrode with the anti-oxidation layer of the silver material, in addition to preventing oxidation, silver has good conductivity and low impedance, so that the speed and accuracy of detection can be relatively increased.

100‧‧‧ fast screening wafer

10‧‧‧ basal layer

20‧‧‧ circuit layer

21‧‧‧First electrode

211‧‧‧First leading end

212‧‧‧First connection segment

22‧‧‧second electrode

221‧‧‧Second guiding end

222‧‧‧Second connection

23‧‧‧ Sensing pole

24‧‧‧ gap

30‧‧‧Antioxidant layer

40‧‧‧Fluorinated layer

50‧‧‧Barrier

51‧‧‧Detection hole

60‧‧‧Reagents

70‧‧‧Mobile reading device

71‧‧‧Connected

72‧‧‧ wafer

80‧‧‧ mobile devices

90‧‧‧National Agricultural Administration Unit

The first figure is a preferred embodiment of the fast screening processing wafer for detecting pesticide residues in fruits and vegetables. An exploded perspective view.

Figure 2 is a combined perspective view of the fast screening wafer.

Figure 3 is a cross-sectional view of the fast screening wafer.

Figure 4 is a schematic diagram of the combined operation of the fast screening wafer.

Referring to FIG. 1 , FIG. 2 and FIG. 3 , a preferred embodiment of the fast-screening wafer 100 for detecting pesticide residues in fruits and vegetables comprises a substrate layer 10 , a circuit layer 20 , and an oxidation resistant layer 30 . , a fluorinated layer 40 and a barrier layer 50.

The base layer 10 is made of plastic material or paper.

The circuit layer 20 is disposed on the base layer 10 and has a first electrode 21, a second electrode 22 and a sensing pole 23. The first electrode 21 is connected to the sensing pole 23, and the second The electrode 22 is spaced apart from the sensing pole 23 and is not connected. In the present embodiment, the circuit layer 20 is made of silver. Therefore, the portion of the circuit wiring of the fast-screening wafer 100 that is exposed in appearance is silver-white, which is not conventional carbon black.

The oxidation resistant layer 30 is overlaid on the circuit layer 20. In this embodiment, the oxidation resistant layer 20 may be cerium oxide to prevent oxidation of the surface of the circuit layer 20 and to reduce electrical conductivity.

The fluorinated layer 40 is a fluorinated polymeric derivative (for example, Nafion) and is overlaid on the oxidation resistant layer 30.

The barrier layer 50 covers the fluorination layer 40, the oxidation resistant layer 30, the circuit layer 20, and the base layer 10, so that part of the first electrode 21 and the second electrode 22 of the circuit layer 20 are exposed. The barrier layer 50 has a detection hole 51 corresponding to the sensing pole 23 of the circuit layer 20.

Further, the first electrode 21 of the circuit layer 20 has a first end located at the front end a first connecting portion 212 extending from the first guiding end 211 and connected to the sensing pole 23, the second electrode 22 having a second guiding end 221 at the front end and A second connecting portion 222 extending outwardly from the second guiding end 221, a gap 24 is left between the second connecting portion 222 of the second electrode 22 and the sensing pole 23, and the detecting hole 51 of the blocking layer 50 Is a first connecting segment 212 corresponding to the first electrode 22 portion, the sensing pole 23, the second connecting portion 222 of the second electrode 22 portion, and the gap 24, so that a portion of the first connecting portion 212, the portion The sensing pole 23, a portion of the second connecting section 222, and the gap 24 are all exposed to the outside, as shown in FIG.

As for the use of the fast-screening wafer for detecting pesticide residues in fruits and vegetables, as shown in FIG. 4, the screen processing wafer 100 is connected to a port 71 of a mobile reading device 70, the mobile reading device. The 70 is wirelessly connected to a mobile device 80 (smartphone, iPad, iPod or laptop) via a Wi-Fi or Bluetooth system. When the user drops the reagent 60 to the detecting hole 51 of the fast processing wafer 100, the reagent 60 falls on the sensing pole 23, and the first electrode 21 and the second electrode 22 are electrically connected to each other across the gap 24, After energizing the fast-screening wafer 100, the reagent 60 is rapidly reacted on the fluorinated layer 40 dropped on Nafion, and the concentration of the reagent 60 is analyzed by plasma chemistry. Therefore, by measuring the potential of the reagent 60 (Potential) , current and resistance (conductivity), through the mobile device 72 built into the mobile device 72 to programmatically calculate the concentration of pesticide residues in the reagent 60, and convert the data content of the concentration of the pesticide 60 pesticide residue After being transmitted to the mobile device 80, an operation is performed through the APP program to process the data, so that the user can immediately know whether the concentration level of the pesticide residue of the reagent 60 has exceeded the standard, and accordingly, the present invention processes the wafer 100 through the fast screening. The purpose of rapid screening of pesticide residues in fruits and vegetables can be achieved.

In addition, the APP program of the mobile device 80 is connected to a national agricultural administrative unit 90 through wireless communication to instantly update the standard information of fruits and vegetables released by the national agricultural administrative unit 90, so that the testing standard is more accurate.

In addition, the mobile reading device 70 in FIG. 4 can also be a wearing device. Or the i watch, the smart watch has all the structures and functions of the aforementioned action reading device 70, which will not be explained. Therefore, wearing a smart watch on the wrist will make it easier to test the pesticides of fruits and vegetables, and the smart watch has the basic watch function. It can also provide the basic watch when it is not necessary to test the pesticides. Time-saving function, and convenient to carry and use.

According to the above, compared with the existing enzyme analysis method for discriminating pesticide residues, the operation is complicated, the detection speed is slow and the impedance is high, resulting in inaccurate detection, and it is necessary to cooperate with professionals and professional equipment technology, and adopt carbon. The measurement of electrode analysis is costly and not universally applicable. The fluorinated polymeric derivative of the fluorinated layer 40 of the wafer 100 is treated by the fast screening method for the rapid sieving treatment of the pesticide residues in the present invention, so that the reagent 60 is dropped on the fluorinated layer 40, and The circuit layer 20 is made conductive, and the wafer electrode is modified by plasma chemical deposition of Nafion and derivatives, and the electrochemical potential interval and redox are professionally analyzed to detect the structure of the pesticide, and to determine the chlorobenzene. The amount of toxic substances is used to obtain high sensitivity detection and excellent reproducibility. Moreover, in the present case, the circuit layer 20 is made of silver, and has high conductivity and low impedance. In addition, the anti-oxidation layer 40 made of ruthenium dioxide is coated on the outer surface of the electrode to prevent oxidation, so that it can be relatively improved. Speed and accuracy in detection.

Therefore, in operation, the fast screening processing chip 100 is electrically connected to the mobile reading device 70, and then the mobile device, the iPad, the iPod, or the notebook computer is used to execute the operation through the APP program. It is quick to know whether the pesticide residue content of fruits and vegetables has exceeded the standard value. It is not only quick and easy to operate, but also can be used without specific professionals. This is a great boon for consumers or health inspectors, plus Nowadays, the convenience brought by the technology era of almost one hand in the society can quickly learn the test results through the APP program of the mobile device 80, so that the detection of pesticide residues in fruits and vegetables can truly achieve rapid screening, and the detection method is simpler. Convenient function.

It is worth mentioning that, because the fast screening processing chip 100, the mobile reading device 70 and the mobile device 80 can be carried around, and the fast screening processing chip 100 can be a disposable test piece, it is easy to take the material. Consumers want to test the pesticide residue content of fruits and vegetables, it will be easier and faster, and through the APP program of mobile device 80, it can instantly know whether the pesticide residue content of fruits and vegetables has exceeded the standard, so as to make food more secure.

The above description is only a preferred embodiment of the present invention, and the scope of the present invention cannot be limited by this, that is, the simple equivalent changes and modifications made by the applicant according to the scope of the patent application and the new description of the creation are all It should remain within the scope of this creation patent.

100‧‧‧ fast screening wafer

10‧‧‧ basal layer

20‧‧‧ circuit layer

21‧‧‧First electrode

211‧‧‧First leading end

212‧‧‧First connection segment

22‧‧‧second electrode

221‧‧‧Second guiding end

222‧‧‧Second connection

23‧‧‧ Sensing pole

24‧‧‧ gap

30‧‧‧Antioxidant layer

40‧‧‧Fluorinated layer

50‧‧‧Barrier

51‧‧‧Detection hole

Claims (7)

A fast screening processing wafer for detecting pesticide residues in fruits and vegetables comprises: a substrate layer; a circuit layer disposed on the substrate layer and having a first electrode, a second electrode and a sensing pole, the first electrode Connected to the sensing pole, the second electrode is not connected to the sensing pole; the fluorinated layer is a fluorinated polymeric derivative covering the circuit layer; a barrier layer covering the The fluorination layer, the circuit layer and the substrate layer are exposed to a portion of the first electrode and the second electrode of the circuit layer, and the barrier layer has a detection hole corresponding to the sensing pole of the circuit layer. The fast-screening wafer for detecting pesticide residues in fruits and vegetables as described in claim 1, wherein the circuit layer is silver, and the portion of the circuit wiring of the fast-screening wafer exposed is silver-white. The fast-screening wafer for detecting pesticide residues in fruits and vegetables as described in claim 1, further comprising an anti-oxidation layer covering the circuit layer to be sandwiched between the circuit layer and the fluorination layer between. The fast-screening wafer for detecting pesticide residues in fruits and vegetables as described in claim 3, wherein the antioxidant layer is cerium oxide. The fast-screening wafer for detecting pesticide residues in fruits and vegetables as described in claim 1, wherein the first electrode has a first guiding end at the front end and extending outward from the first guiding end Sensing a first connecting segment connected to the pole, the second electrode having a second guiding end at the front end and a second connecting portion extending outward from the second guiding end, the second connection of the second electrode A gap remains between the segment and the sensing pole, and the detecting hole of the blocking layer is a first connecting segment corresponding to the first electrode portion, the sensing pole, the second connecting segment of the second electrode portion, and the The gap is such that a portion of the first connecting segment, the sensing pole, a portion of the second connecting segment, and the gap are exposed to the outside. The fast-screening wafer for detecting pesticide residues in fruits and vegetables as described in claim 1, wherein the substrate layer is made of plastic material or paper. The fast-screening wafer for detecting pesticide residues in fruits and vegetables as described in claim 1, wherein the fast-screening wafer is inserted into a port of a mobile reading device to detect the value of pesticide residues.