EP4433605A4 - Procédés de synthèse de molécules d'acide nucléique - Google Patents

Procédés de synthèse de molécules d'acide nucléique

Info

Publication number: EP4433605A4
Authority: EP; European Patent Office
Prior art keywords: synthesis; processes; nucleic acid; acid molecules; molecules
Prior art date: 2021-11-15
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

EP21964265.9A

Other languages

German (de)

English (en)

Other versions

EP4433605A1 (fr

Inventor

John E. Gill

Lixia Fu

Sydney KERR

Michelle VARGAS

Daniel G. Gibson

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Telesis Bio Inc

Original Assignee

Telesis Bio Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2021-11-15

Filing date

2021-11-15

Publication date

2025-01-08

2021-11-15 Application filed by Telesis Bio Inc filed Critical Telesis Bio Inc

2024-09-25 Publication of EP4433605A1 publication Critical patent/EP4433605A1/fr

2025-01-08 Publication of EP4433605A4 publication Critical patent/EP4433605A4/fr

Status Pending legal-status Critical Current

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Biotechnology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Crystallography & Structural Chemistry (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Bioinformatics & Computational Biology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

EP21964265.9A 2021-11-15 2021-11-15 Procédés de synthèse de molécules d'acide nucléique Pending EP4433605A4 (fr)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
PCT/US2021/059422 WO2023086109A1 (fr)	2021-11-15	2021-11-15	Procédés de synthèse de molécules d'acide nucléique

Publications (2)

Publication Number	Publication Date
EP4433605A1 EP4433605A1 (fr)	2024-09-25
EP4433605A4 true EP4433605A4 (fr)	2025-01-08

Family

ID=86336336

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP21964265.9A Pending EP4433605A4 (fr)	2021-11-15	2021-11-15	Procédés de synthèse de molécules d'acide nucléique

Country Status (5)

Country	Link
EP (1)	EP4433605A4 (fr)
CN (1)	CN118355127A (fr)
AU (1)	AU2021473848A1 (fr)
CA (1)	CA3238653A1 (fr)
WO (1)	WO2023086109A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US20250346886A1 (en) *	2024-05-09	2025-11-13	Telesis Bio, Inc.	Methods of Synthesizing Nucleic Acid Molecules

Citations (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2018111914A1 (fr) *	2016-12-14	2018-06-21	Synthetic Genomics, Inc.	Procédés d'assemblage de molécules d'adn
US20190169665A1 (en) *	2015-01-22	2019-06-06	Mlp Holding Aps	Gene Synthesis by Self-Assembly of Small Oligonucleotide Building Blocks

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US9187777B2 (en) *	2010-05-28	2015-11-17	Gen9, Inc.	Methods and devices for in situ nucleic acid synthesis
EP4114936A4 (fr) *	2020-03-03	2024-03-27	Codex Dna, Inc.	Procédés pour l'assemblage d'acides nucléiques

2021
- 2021-11-15 CA CA3238653A patent/CA3238653A1/fr active Pending
- 2021-11-15 CN CN202180104210.3A patent/CN118355127A/zh active Pending
- 2021-11-15 WO PCT/US2021/059422 patent/WO2023086109A1/fr not_active Ceased
- 2021-11-15 EP EP21964265.9A patent/EP4433605A4/fr active Pending
- 2021-11-15 AU AU2021473848A patent/AU2021473848A1/en active Pending

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US20190169665A1 (en) *	2015-01-22	2019-06-06	Mlp Holding Aps	Gene Synthesis by Self-Assembly of Small Oligonucleotide Building Blocks
WO2018111914A1 (fr) *	2016-12-14	2018-06-21	Synthetic Genomics, Inc.	Procédés d'assemblage de molécules d'adn

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHAO RAN ET AL: "Recent advances in DNA assembly technologies", vol. 15, no. 1, February 2015 (2015-02-01), GB, NL, pages 1 - 9, XP055917292, ISSN: 1567-1356, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4257898/pdf/12171.pdf> DOI: 10.1111/1567-1364.12171 *
See also references of WO2023086109A1 *

Also Published As

Publication number	Publication date
EP4433605A1 (fr)	2024-09-25
CA3238653A1 (fr)	2023-05-19
WO2023086109A1 (fr)	2023-05-19
CN118355127A (zh)	2024-07-16
AU2021473848A1 (en)	2024-06-20

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Legal Events

Date	Code	Title	Description
2023-05-19	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE
2024-08-22	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2024-08-22	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE
2024-09-25	17P	Request for examination filed	Effective date: 20240524
2024-09-25	AK	Designated contracting states	Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
2024-12-02	REG	Reference to a national code	Ref country code: DE Ref legal event code: R079 Free format text: PREVIOUS MAIN CLASS: C12Q0001680600 Ipc: C12N0015100000
2025-01-08	A4	Supplementary search report drawn up and despatched	Effective date: 20241206
2025-01-08	RIC1	Information provided on ipc code assigned before grant	Ipc: C12P 19/34 20060101ALI20241202BHEP Ipc: C12N 15/10 20060101AFI20241202BHEP
2025-02-19	DAV	Request for validation of the european patent (deleted)
2025-02-19	DAX	Request for extension of the european patent (deleted)
2025-10-02	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: EXAMINATION IS IN PROGRESS
2025-11-05	17Q	First examination report despatched	Effective date: 20251001