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EP3060683A4 - Methods and apparatus for point-of-care nucleic acid amplification and detection - Google Patents

Methods and apparatus for point-of-care nucleic acid amplification and detection Download PDF

Info

Publication number
EP3060683A4
EP3060683A4 EP14856081.6A EP14856081A EP3060683A4 EP 3060683 A4 EP3060683 A4 EP 3060683A4 EP 14856081 A EP14856081 A EP 14856081A EP 3060683 A4 EP3060683 A4 EP 3060683A4
Authority
EP
European Patent Office
Prior art keywords
detection
methods
point
nucleic acid
acid amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP14856081.6A
Other languages
German (de)
French (fr)
Other versions
EP3060683A1 (en
Inventor
Jane P. Bearinger
Scott Castanon
Kenneth J. Michlitsch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Corporos Inc
Original Assignee
Corporos Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US14/262,683 external-priority patent/US9469871B2/en
Application filed by Corporos Inc filed Critical Corporos Inc
Publication of EP3060683A1 publication Critical patent/EP3060683A1/en
Publication of EP3060683A4 publication Critical patent/EP3060683A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0672—Integrated piercing tool
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0874—Three dimensional network
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0605—Valves, specific forms thereof check valves
    • B01L2400/0616—Ball valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/065—Valves, specific forms thereof with moving parts sliding valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
EP14856081.6A 2013-10-22 2014-10-22 Methods and apparatus for point-of-care nucleic acid amplification and detection Withdrawn EP3060683A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361894392P 2013-10-22 2013-10-22
US14/262,683 US9469871B2 (en) 2011-04-14 2014-04-25 Methods and apparatus for point-of-care nucleic acid amplification and detection
PCT/US2014/061808 WO2015061480A1 (en) 2013-10-22 2014-10-22 Methods and apparatus for point-of-care nucleic acid amplification and detection

Publications (2)

Publication Number Publication Date
EP3060683A1 EP3060683A1 (en) 2016-08-31
EP3060683A4 true EP3060683A4 (en) 2017-08-09

Family

ID=52993515

Family Applications (1)

Application Number Title Priority Date Filing Date
EP14856081.6A Withdrawn EP3060683A4 (en) 2013-10-22 2014-10-22 Methods and apparatus for point-of-care nucleic acid amplification and detection

Country Status (5)

Country Link
EP (1) EP3060683A4 (en)
JP (1) JP2016539633A (en)
CN (1) CN105874084A (en)
CA (1) CA2928406A1 (en)
WO (1) WO2015061480A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106010961B (en) * 2016-08-03 2019-11-22 珠海百瑞生物科技有限公司 High-throughput ring mediated isothermal amplification identification food borne pathogens body device and detection method
EP3342485B1 (en) * 2017-01-02 2020-07-08 Thinxxs Microtechnology Ag Holder for reagent elements
CA3057326A1 (en) 2017-03-22 2018-09-27 The Board Of Trustees Of The University Of Illinois System for rapid, portable, and multiplexed detection and identification of pathogen specific nucleic acid sequences
EP3606668A1 (en) * 2017-04-04 2020-02-12 Selfdiagnostics Deutschland GmbH Lamp component distribution in a microfluid cell
US11547997B2 (en) 2017-04-07 2023-01-10 Arizona Board Of Regents On Behalf Of Arizona State University Integrated diagnostic devices having embedded biomolecular computing systems and uses thereof
CN107129930B (en) * 2017-06-09 2019-11-26 北京百康芯生物科技有限公司 A kind of fully integrated detection of nucleic acids micro-fluidic chip and its application method
DE102017125881B4 (en) * 2017-11-06 2019-06-19 Sartorius Stedim Biotech Gmbh Filter module and method for detecting microorganisms
CN108642148B (en) * 2018-07-09 2024-01-30 南京岚煜生物科技有限公司 Nucleic acid amplification detection micro-fluidic chip and detection method thereof
CN112852928A (en) * 2021-01-14 2021-05-28 广东东阳光药业有限公司 Multiplex nucleic acid amplification product detection method and kit
US20220235394A1 (en) * 2021-01-25 2022-07-28 Sandra Janeth Perdomo Lara Reverse transcription polymerase chain reaction diagnostic testing device
JP7728564B2 (en) * 2021-05-11 2025-08-25 国立大学法人 東京大学 Virus detection method and virus detection device
KR102796383B1 (en) * 2022-03-08 2025-04-18 주식회사 유진셀 Test cartridge

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006018044A1 (en) * 2004-08-18 2006-02-23 Agilent Technologies, Inc. Microfluidic assembly with coupled microfluidic devices
WO2007011867A2 (en) * 2005-07-15 2007-01-25 Applera Corporation Fluid processing device and method
WO2011097412A1 (en) * 2010-02-03 2011-08-11 President And Fellows Of Harvard College Devices and methods for multiplexed assays

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006122311A2 (en) * 2005-05-11 2006-11-16 The Trustees Of The University Of Pennsylvania Microfluidic chip
WO2007106579A2 (en) * 2006-03-15 2007-09-20 Micronics, Inc. Integrated nucleic acid assays
WO2008147382A1 (en) * 2006-09-27 2008-12-04 Micronics, Inc. Integrated microfluidic assay devices and methods
JP6181370B2 (en) * 2010-02-12 2017-08-16 ノースウェスタン ユニバーシティ Assay card for sample acquisition, processing and reaction
US8911941B2 (en) * 2011-04-14 2014-12-16 Kenneth J. Michlitsch Methods and apparatus for point-of-care nucleic acid amplification and detection

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006018044A1 (en) * 2004-08-18 2006-02-23 Agilent Technologies, Inc. Microfluidic assembly with coupled microfluidic devices
WO2007011867A2 (en) * 2005-07-15 2007-01-25 Applera Corporation Fluid processing device and method
WO2011097412A1 (en) * 2010-02-03 2011-08-11 President And Fellows Of Harvard College Devices and methods for multiplexed assays

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2015061480A1 *

Also Published As

Publication number Publication date
EP3060683A1 (en) 2016-08-31
WO2015061480A1 (en) 2015-04-30
CN105874084A (en) 2016-08-17
CA2928406A1 (en) 2015-04-30
JP2016539633A (en) 2016-12-22

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