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Prey et al., 2001 - Google Patents

two distinct domains: a DNA-binding (Gal4pDNA) and an acidic transactivation domain (Gal4pTA). A functional transcription factor is restored when the separated …

Prey et al., 2001

Document ID
8203800984864586574
Author
Prey P
Schramm L
Publication year
Publication venue
Centrosomes and Spindle Pole Bodies

External Links

Snippet

GALUAS. Several two-hybrid vectors (Table I) as well as yeast strains with different reporter constructs (LacZ, HIS3, ADE2) have been constructed. Some two-hybrid plasmids use DNA- binding (bait plasmids) or transactivation domains (prey plasmids) from other proteins, eg …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
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    • G01—MEASURING; TESTING
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • C12N15/09—Recombinant DNA-technology
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