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MAP Kinase Signaling Protocols

  • Book
  • © 2004

Overview

Editors:
  1. Rony Seger

    1. Department of Biological Regulation, The Zeizmann Institute of Science, Rehovot, Israel

Part of the book series: Methods in Molecular Biology (MIMB, volume 250)

  • 20k Accesses

  • 2 Citations

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About this book

Mitogen-activated protein kinase (MAPK) signaling cascades are a group of protein kinases that play a central role in the intracellular transmission of extracellular signals. These cascades operate as major lines of communication within a complicated signaling network that regulates many cellular processes, including proliferation, differentiation, development, stress response, and apoptosis. More than 15,000 papers on MAPKs have been published over the past few years, with the number of publications increasing each year. More and more laboratories embark on the study of MAPK cascades in many d- tinct cellular systems and in particular their role in disease. Future challenges in the study of MAPK cascades remain in understa- ing the role of the various components and isoforms of the cascades in the multiple critical functions that they regulate in the whole organism, as well as the diseases caused by their malfunction. Data from gene-disrupted mice s- gest that inhibition of the MAPK cascades may have serious consequences on the development and growth of the animals. For example, targeted deletion of MEK1 is lethal, owing to developmental problems of placental vasculature and abnormal fibroblast migration. This lethality occurs in spite of the normal expression of MEK2, indicating that although the two MEK isoforms are apparently similar, they do have distinct functions, at least during embryog- esis. The ERK cascade was also shown to play a central role in brain function and in learning and memory.

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. The ERK Cascade As a Prototype of MAPK Signaling Pathways

    • Hadara Rubinfeld, Rony Seger
    Pages 1-28

  3. Determination of ERK Activity

    • Sarah Kraus, Rony Seger
    Pages 29-47

  4. Detection of ERK1/2 Activities Using Affinity Reagents

    • Rafael Pulido, Ángel Zúñiga, Axel Ullrich
    Pages 49-60

  5. Activation of SAPKs/JNKs and p38s In Vitro

    • John M. Kyriakis, Hong Liu, Deborah N. Chadee
    Pages 61-88

  6. Investigating the Cellular BMK1/ERK5 Signaling Pathway

    • Richard I. Tapping, Kato Yutaka, Ta-Hsiang Chao, Masaaki Hayashi, Jeng-Fan Lo, Sung-Woo Kim et al.
    Pages 89-96

  7. Pull-Down Assays for Guanosine 5′-Triphosphate-Bound Ras-Like Guanosine 5′-Triphosphatases

    • Miranda van Triest, Johannes L. Bos
    Pages 97-102

  8. Regulation of MAPK Cascades by Protein Tyrosine Phosphatases

    • Josema Torres, Carmen Blanco-Aparicio, Rafael Pulido
    Pages 103-112

  9. Use of Inhibitors in the Study of MAPK Signaling

    • Yoav D. Shaul, Rony Seger
    Pages 113-125

  10. Structure of MAPKs

    • Elizabeth J. Goldsmith, Melanie H. Cobb, Chung-I Chang
    Pages 127-143

  11. Subcellular Localization of MAPKs

    • Makoto Adachi, Eisuke Nishida
    Pages 145-153

  12. Study of MAPK Signaling Using Knockout Mice

    • Gilles Pagès, Jacques Pouysségur
    Pages 155-166

  13. Computer Simulation of MAPK Signal Transduction

    • Baltazar D. Aguda, Herbert M. Sauro
    Pages 167-175

  14. Signaling by Growth Factor Receptors

    • Bose S. Kochupurakkal, Yosef Yarden
    Pages 177-202

  15. Activation of MAPKs by G Protein-Coupled Receptors

    • Piero Crespo, J. Silvio Gutkind
    Pages 203-210

  16. Identification of MAPK Substrates by Expression Screening With Solid-Phase Phosphorylation

    • Rikiro Fukunaga, Tony Hunter
    Pages 211-236

  17. Study of Substrate Specificity of MAPKs Using Oriented Peptide Libraries

    • Michael B. Yaffe
    Pages 237-249

  18. Ras Signaling Pathway for Analysis of Protein-Protein Interactions in Yeast and Mammalian Cells

    • Ami Aronheim
    Pages 251-261

  19. Methods in Functional Proteomics

    • Karine R. Bernard, Karen R. Jonscher, Katheryn A. Resing, Natalie G. Ahn
    Pages 263-281

  20. Practical Methods for Deuterium Exchange/Mass Spectrometry

    • Andrew N. Hoofnagle, Katheryn A. Resing, Natalie G. Ahn
    Pages 283-298
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Reviews

...this book continues the strong tradition of the series by recruiting the most prominent investigators in the field to provide a detailed discussion of specialized topics with a focus on direct practical applications.-Doody's Health Sciences Book Review Journal

Editors and Affiliations

  • Department of Biological Regulation, The Zeizmann Institute of Science, Rehovot, Israel

    Rony Seger

Accessibility Information

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Bibliographic Information

  • Book Title: MAP Kinase Signaling Protocols

  • Editors: Rony Seger

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592596711

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Humana Press 2004

  • eBook ISBN: 978-1-59259-671-3Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 332

  • Topics: Biochemistry, general

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