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- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 216)
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About this book
The polymerase chain reaction (PCR) has become an increasingly important tool in microbial diagnosis in recent years, mainly because of its rapidity, high sensitivity, and specificity. In a number of instances, parti- larly with noncultivatable and slow-growing microorganisms, reliable iden- fication and differentiation became possible only after the introduction of PCR-based methods. Because the number of published procedures is large and steadily rising, there is a need for critical evaluation, comparison of performance, and even- ally also standardization of methods to enable laboratory diagnosticians to select the optimal methodology. PCR Detection of Microbial Pathogens is designed to provide an ov- view of the possibilities and problems connected with the use of PCR-based identification and detection of important bacterial and other microbial pat- gens, among them several zoonotic agents. It is intended to provide micro- ologists and biochemists the opportunity to extend their knowledge of state-of-the-art detection procedures, as well as pre-PCR sample processing and various general aspects of PCR. The chapters of this volume have been written by investigators well conversant with molecular detection and iden- fication of microorganisms. They have contributed their extensive practical experience to the protocols, not only concerning PCR methodology itself, but also the highly important area of pre-amplification sample processing.
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Table of contents (22 protocols)
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Front Matter
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Protocols
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Front Matter
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Reviews
"...PCR Detection of Microbial Pathogens is useful, comprehensive and practical guide for everyone who wants to use PCR for detection of the above mentioned pathogens and, at the same time, an inspiration for development of new PCR detection systems." -Folia Microbiologica
Editors and Affiliations
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Bibliographic Information
Book Title: PCR Detection of Microbial Pathogens
Editors: Konrad Sachse, Joachim Frey
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1385/1592593445
Publisher: Humana Totowa, NJ
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eBook Packages: Springer Book Archive
Copyright Information: Humana Press 2003
eBook ISBN: 978-1-59259-344-6Published: 02 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 336
Topics: Microbiology