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DNA Methylation Protocols

  • Book
  • © 2002

Overview

Editors:
  1. Ken I. Mills

    1. Department of Haematology, University of Wales College of Medicine, Cardiff, UK

  2. Bernard H. Ramsahoye

    1. Department of Haematology, Western General Hospital, Edinburgh, UK

Part of the book series: Methods in Molecular Biology (MIMB, volume 200)

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About this book

DNA Methylation Protocols offer a set of readily reproducible protocols of the analysis of DNA methylation and methylases. These powerful methods provide the tools necessary for studying methylation at both the global level and the level of sequence, and include many techniques for identifying genes that might be aberrantly methylated in cancer and aging. Additional methods cover genome-wide analysis of abnormal DNA methylation and the isolation and measurement of demethylases and related proteins.

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Overview

    • Ken I. Mills, Bernard H. Ramsahoye
    Pages 1-8

  3. Nearest-Neighbor Analysis

    • Bernard H. Ramsahoye
    Pages 9-16

  4. Measurement of Genome-Wide DNA Cytosine-5 Methylation by Reversed-Phase High-Pressure Liquid Chromatography

    • Bernard H. Ramsahoye
    Pages 17-27

  5. Methylation Analysis by Chemical DNA Sequencing

    • Piroska E. Szabó, Jeffrey R. Mann, Gerd P. Pfeifer
    Pages 29-42

  6. Methylation-Sensitive Restriction Fingerprinting

    • Catherine S. Davies
    Pages 43-52

  7. Restriction Landmark Genome Scanning

    • Joseph F. Costello, Christoph Plass, Webster K. Cavenee
    Pages 53-70

  8. Combined Bisulfite Restriction Analysis (COBRA)

    • Cindy A. Eads, Peter W. Laird
    Pages 71-86

  9. Differential Methylation Hybridization Using CpG Island Arrays

    • Pearlly S. Yan, Susan H. Wei, Tim Hui-sing Huang
    Pages 87-100

  10. Methylated CpG Island Amplification for Methylation Analysis and Cloning Differentially Methylated Sequences

    • Minoru Toyota, Jean-Pierre J. Issa
    Pages 101-110

  11. Isolation of CpG Islands Using a Methyl-CpG Binding Column

    • Sally H. Cross
    Pages 111-130

  12. Purification of MeCP2-Containing Deacetylase from Xenopus laevis

    • Peter L. Jones, Paul A. Wade, Alan P. Wolffe
    Pages 131-141

  13. DNA-Methylation Analysis by the Bisulfite-Assisted Genomic Sequencing Method

    • Petra Hajkova, Osman El-Maarri, Sabine Engemann, Joachim Oswald, Alexander Olek, Jörn Walter
    Pages 143-154

  14. Measuring DNA Demethylase Activity In Vitro

    • Moshe Szyf, Sanjoy K. Bhattacharya
    Pages 155-161

  15. Extracting DNA Demethylase Activity from Mammalian Cells

    • Moshe Szyf, Sanjoy K. Bhattacharya
    Pages 163-176

  16. Back Matter

    Pages 177-189
    Download chapter PDF
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Editors and Affiliations

  • Department of Haematology, University of Wales College of Medicine, Cardiff, UK

    Ken I. Mills

  • Department of Haematology, Western General Hospital, Edinburgh, UK

    Bernard H. Ramsahoye

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Bibliographic Information

  • Book Title: DNA Methylation Protocols

  • Editors: Ken I. Mills, Bernard H. Ramsahoye

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592591825

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Humana Press 2002

  • eBook ISBN: 978-1-59259-182-4Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 189

  • Topics: Biochemistry, general

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