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- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 173)
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About this book
Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.
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Table of contents (29 protocols)
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Front Matter
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Methods and Techniques to Study
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Front Matter
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Editors and Affiliations
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Bibliographic Information
Book Title: Calcium-Binding Protein Protocols
Book Subtitle: Volume 2: Methods and Techniques
Editors: Hans J. Vogel
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1385/1592591841
Publisher: Humana Totowa, NJ
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eBook Packages: Springer Book Archive
Copyright Information: Humana Press 2002
Hardcover ISBN: 978-0-89603-689-5Published: 24 January 2002
Softcover ISBN: 978-1-61737-136-3Published: 10 November 2010
eBook ISBN: 978-1-59259-184-8Published: 05 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVII, 415
Topics: Biochemistry, general