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Yersinia Pestis Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Ruifu Yang

    1. Beijing Institute of Microbiology and Epidemiology, Beijing, China

  • Comprehensively summarizing common and advanced research protocols for Yersinia pestis research
  • Including protocols for genomic and transcriptomic analysis, small RNA study, protein-protein interaction, immune response assays and animal models
  • Providing online resources for Yersinia pestis research

Part of the book series: Springer Protocols Handbooks (SPH)

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About this book

This protocol book includes common and advanced research protocols for Yersinia pestis research and for its identification, genotyping and source-tracing. This book includes protocols for genomic and transcriptomic analysis, small RNA study, protein-protein interaction, gene deletion. It will be helpful for graduate students, clinicians, and researchers in infectious diseases, systems biology, and evolution and numerous other relevant fields.

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-vi
    Download chapter PDF

  2. Isolation and Identification of Yersinia pestis

    • Zhizhen Qi, Qingwen Zhang, Yong Jin, Youquan Xin, Haihong Zhao, Xiaoyan Yang et al.
    Pages 1-14

  3. Isolation and Characterization of Yersinia pestis Bacteriophages

    • Xiangna Zhao
    Pages 15-22

  4. Extraction of Chromosomal DNA and Plasmid from Yersinia pestis

    • Yazhou Zhou
    Pages 23-27

  5. Extraction of Total RNA from Yersinia pestis

    • Haili Wang, Yanping Han
    Pages 29-34

  6. Genotyping of Yersinia pestis

    • Yanjun Li, Yujun Cui
    Pages 35-59

  7. Genome-Wide Variation Analysis of Yersinia pestis

    • Chao Yang, Yujun Cui
    Pages 61-66

  8. Genome-Wide Detection of Expressed sRNAs in Yersinia pestis with RNA-seq

    • Yanping Han
    Pages 67-80

  9. Validation of Yersinia pestis sRNAs with Nonradioactive-Labeled Northern Blotting

    • Yanping Han
    Pages 81-88

  10. Determination of Transcription Start Sites (TSSs) in Yersinia pestis with a Primer Extension Assay

    • Yanping Han
    Pages 89-98

  11. Purification of Hfq-Associated RNAs with RNA Immunoprecipitation (RIP)

    • Yanping Han
    Pages 99-106

  12. Overexpression of Target sRNAs in Tightly Controlled Multicopy Plasmids

    • Xiaofang Gao, Zizhong Liu, Yanping Han
    Pages 107-113

  13. Target Validation of sRNA with a GFP Reporter Gene Fusion System

    • Xiaofang Gao, Zizhong Liu, Yanping Han
    Pages 115-120

  14. Inducible Gene Expression in Yersinia pestis

    • Juping Zhao, Yicheng Sun
    Pages 121-126

  15. Analysis of Yops Secretion by Yersinia pestis Type III Secretion System

    • Zongmin Du, Shiyang Cao
    Pages 127-132

  16. Co-immunoprecipitation Analysis for the Detection of Protein–Protein Interactions in Yersinia pestis

    • Shiyang Cao, Zongmin Du
    Pages 133-138

  17. Detection of Type III Secretion System Needle Assembly in Yersinia pestis by Cross-Linking YscF

    • Shiyang Cao, Zongmin Du
    Pages 139-143

  18. Evaluation of Protein Translocation into Eukaryotic Cells by Yersinia pestis

    • Yafang Tan
    Pages 145-150

  19. Adhesion Analysis of Yersinia pestis to Host Cells

    • Yafang Tan
    Pages 151-156

  20. Construction of Gene Deletion Mutants in Yersinia pestis

    • Wenliang Li, Dan Rong, Yanping Han
    Pages 157-163
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Editors and Affiliations

  • Beijing Institute of Microbiology and Epidemiology, Beijing, China

    Ruifu Yang

About the editor

Dr. Ruifu Yang is a professor at the Beijing Institute of Microbiology and Epidemiology and the Chief-Scientist for National Basic Research Program (973 Program). He serves as the vice president for the Chinese Society for Microbiology. His research focuses on bacterial pathogenesis using genomics- and proteomics-based techniques. He has published more than 100 articles on international well-known journals, such as Nature Genetics, PNAS, BMC Microbiology.  

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Bibliographic Information

  • Book Title: Yersinia Pestis Protocols

  • Editors: Ruifu Yang

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-981-10-7947-4

  • Publisher: Springer Singapore

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Nature Singapore Pte Ltd. 2018

  • Hardcover ISBN: 978-981-10-7946-7Published: 12 June 2018

  • Softcover ISBN: 978-981-13-4024-6Published: 29 December 2018

  • eBook ISBN: 978-981-10-7947-4Published: 31 May 2018

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: VI, 290

  • Number of Illustrations: 26 b/w illustrations, 16 illustrations in colour

  • Topics: Microbiology

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