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About this book
DNA Methods in Clinical Microbiology describes the novel DNA-based technology now used in the diagnosis and management of infectious diseases. It is a concise, yet readable, overview written primarily for clinicians, clinical microbiologists, medical students and undergraduates in medical and veterinary microbiology.
The book has two primary aims. First, to explain the principles of these methods at the `molecular' level. Second, to provide a clinical perspective by reporting results from actual DNA-based investigations on a range of specimens.
Those approaching DNA methods for the first time are assisted by a brief résumé of the relevant features of nucleic acids (Chapter 2): this information is essential for an understanding of later chapters. Subsequent text covers detection, characterization and quantification of pathogens by a variety of methods - e.g., target amplification (PCR, LCR, NASBA, TMA and SDA), signal amplification (bDNA) and probe-based techniques; the chapter on typing describes nearly twenty named molecular methods, including spoligotyping and MLST. All chapters include an adequate range of current reference from which, if required, detailed protocols can be obtained. The diagrams are clear, and readers are assisted by a detailed index.
The book has two primary aims. First, to explain the principles of these methods at the `molecular' level. Second, to provide a clinical perspective by reporting results from actual DNA-based investigations on a range of specimens.
Those approaching DNA methods for the first time are assisted by a brief résumé of the relevant features of nucleic acids (Chapter 2): this information is essential for an understanding of later chapters. Subsequent text covers detection, characterization and quantification of pathogens by a variety of methods - e.g., target amplification (PCR, LCR, NASBA, TMA and SDA), signal amplification (bDNA) and probe-based techniques; the chapter on typing describes nearly twenty named molecular methods, including spoligotyping and MLST. All chapters include an adequate range of current reference from which, if required, detailed protocols can be obtained. The diagrams are clear, and readers are assisted by a detailed index.
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Table of contents (9 chapters)
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Front Matter
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Back Matter
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Bibliographic Information
Book Title: DNA Methods in Clinical Microbiology
Authors: Paul Singleton
DOI: https://doi.org/10.1007/978-94-017-1286-6
Publisher: Springer Dordrecht
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eBook Packages: Springer Book Archive
Copyright Information: Springer Science+Business Media Dordrecht 2000
Hardcover ISBN: 978-0-7923-6307-1Published: 31 May 2000
Softcover ISBN: 978-90-481-5456-2Published: 01 December 2010
eBook ISBN: 978-94-017-1286-6Published: 17 April 2013
Edition Number: 1
Number of Pages: VIII, 256
Topics: Medical Microbiology, Laboratory Medicine, Veterinary Medicine/Veterinary Science, Biochemistry, general