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PCR Topics

Usage of Polymerase Chain Reaction in Genetic and Infectious Diseases

  • Book
  • © 1991

Overview

Editors:
  1. Arndt Rolfs

    1. Neurologische Klinik des Klinikum Steglitz, FU Berlin, Berlin 45, Germany

  2. Hermann Christian Schumacher

    1. Neurologische Klinik des Klinikum Steglitz, FU Berlin, Berlin 45, Germany

  3. Peter Marx

    1. Neurologische Klinik des Klinikum Steglitz, FU Berlin, Berlin 45, Germany

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About this book

PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines and mainly the broad field of degenerative and inflammatory diseases of nervous system. PCR seems to be the technique which could overcome the two most important problems in that field: very small amount of material combined with the necessity of rapid diagnostic procedures in inflammatory infections. "PCR topics" will give an actual overview of basic and applied research fields on usage of polymerase chain reaction. All contributions to this book have been presented at an international congress on "Usage of Polymerase chain reaction in genetic and infectious diseases" which took place in june 1990 in Berlin. The editors wish to thank all participants for their contributions. We offer our thanks and gratitude to our coworkers and especially to our technical assistents Barbara Trampenau, Mirjana Wiirdemann and Hannelore Leonhard.

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Table of contents (43 chapters)

  1. Front Matter

    Pages I-XI
    Download chapter PDF

  2. Basic Methodology and Research Applications

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Quantitation of mRNA by the Polymerase Chain Reaction

      • Alice M. Wang, Michael V. Doyle, David F. Mark
      Pages 3-8

    3. Sequencing of PCR Products — Analysis of Factor IX Genes and of Recombination Events in Immunoglobulin Genes

      • Jean-Marie Buerstedde, Steve S. Sommer
      Pages 9-14

    4. Alternative Methods for DNA Probing in Diagnosis: Ligase Chain Reaction (LCR)

      • Hartmut Hampl, Ronald A. Marshall, Timothy Perko, Natalie Solomon
      Pages 15-22

    5. Application of PCR to the Detection and Analysis of Point Mutations in the Human Factor VIII Gene

      • Lutz-Peter Berg, David S. Millar, Catherine B. Grundy, Kerstin Wieland, Jonathan K. Pattinson, Edward G. D. Tuddenham et al.
      Pages 23-31

    6. The Use of PCR in the Diagnosis of Muscle Disease

      • Jörg Ehrenpreis, Barbara Junkes, Martin Pfordt, Hans-Peter Vosberg
      Pages 32-40

    7. Taq DNA Polymerase-Synthesized Single-Stranded DNA Hybridization Probes and their Application in Northern Blotting and in situ Hybridization

      • Michael Stürzl, Willy Kurt Roth, Petra Viehweger, Peter-Hans Hofschneider
      Pages 41-45

    8. Computer Program for the Selection of PCR Primers

      • G. Röllich, M. Jafari
      Pages 46-47

    9. Nervous System Cross Reactivity Sequences

      • M. E. Westarp, B. Föhring
      Pages 48-52

    10. The Use of Short Adapters for Priming PCR of Unknown Chromosomal DNA Fragments

      • J. Schneider, H. Schrempf
      Pages 53-55

    11. Labeling of Specific DNA Sequences with Digoxigenin during Polymerase Chain Reaction

      • Rüdiger Rüger, Hans-Joachim Höltke, Udo Reischl, Gregor Sagner, Christoph Kessler
      Pages 56-58

    12. Quantitative Analysis of Polymerase Chain Reaction (PCR) Products by Means of Fluorescence Detection

      • Axel Landgraf, Alfred Pingoud, Bernd Reckmann
      Pages 59-62

    13. Reverse Transcription PCR (RT-PCR) of Parathyroid Hormone (PTH) mRNA

      • Oliva Handt, André Reis, Rudolf Häring, Jörg Schmidtke
      Pages 63-65

    14. False Positive Reactions in PCR

      • Erik C. Böttger
      Pages 66-68

    15. Error Production and Error Propagation During PCR

      • Armin Wagner, Bernd Reckmann, Kerstin Hagen-Mann, Gerhard Krauss
      Pages 69-71
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Editors and Affiliations

  • Neurologische Klinik des Klinikum Steglitz, FU Berlin, Berlin 45, Germany

    Arndt Rolfs, Hermann Christian Schumacher, Peter Marx

Accessibility Information

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Bibliographic Information

  • Book Title: PCR Topics

  • Book Subtitle: Usage of Polymerase Chain Reaction in Genetic and Infectious Diseases

  • Editors: Arndt Rolfs, Hermann Christian Schumacher, Peter Marx

  • DOI: https://doi.org/10.1007/978-3-642-75924-6

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1991

  • Softcover ISBN: 978-3-540-52934-7Published: 19 February 1991

  • eBook ISBN: 978-3-642-75924-6Published: 01 December 2013

  • Edition Number: 1

  • Number of Pages: XI, 258

  • Number of Illustrations: 50 b/w illustrations

  • Topics: Allergology, Immunology, Virology, Medical Microbiology

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