Overview
- Features in-depth protocols on detecting, isolating, characterizing QS signals, function determination, and QS disruption strategies
- Contributions from expert researchers who have experienced the field from its beginnings to the current cutting-edge studies
- Includes key notes on avoiding known protocol pitfalls and troubleshooting potential problems
Part of the book series: Methods in Molecular Biology (MIMB, volume 692)
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About this book
Since its early days in the 1990s, the Quorum Sensing (QS) field has grown from a few dozen laboratories, investigating the pathways, proteins, and chemicals that facilitate signaling in bacteria, to hundreds of groups that have integrated evolutionary biology, computer science, mathematics, engineering, and metagenomics to create an ever-expanding and dynamic field. In Quorum Sensing: Methods and Protocols, expert researchers provide an in-depth set of diverse protocols that span this broad area of study. Broken into three detailed sections, the volume covers the detection, isolation, and characterization of the QS signals made by both Gram- and Gram+ bacteria, determination of the function of QS signals in vivo, and the development of QS disruption strategies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known experimental pitfalls.
Comprehensive and cutting-edge, Quorum Sensing: Methods and Protocols serves as an invaluable collection of easily accessible techniques for scientists seeking to further our knowledge about bacterial communication and its relation to humanity.
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Table of contents (22 protocols)
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Front Matter
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Detection, Isolation, and Characterization of Quorum Sensing Compounds
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Front Matter
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Determining the Function of Autoinducers In vivo
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Front Matter
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Editors and Affiliations
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Bibliographic Information
Book Title: Quorum Sensing
Book Subtitle: Methods and Protocols
Editors: Kendra P. Rumbaugh
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-971-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-60761-970-3Published: 05 November 2010
Softcover ISBN: 978-1-4939-6162-7Published: 23 August 2016
eBook ISBN: 978-1-60761-971-0Published: 29 October 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 315
Topics: Microbiology, Bacteriology, Laboratory Medicine