Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2961)
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About this book
This volume explores the latest advancements in the field of Nuclear Magnetic Resonance (NMR) and discusses how new studies provide insight into the structure, conformation, dynamics, and interactions of glycoproteins. The chapters in this book covers topics such as the preparation of isotope-labeled eukaryotic glycoproteins; applications of paramagnetic NMR to dissect glycosaminoglycan-protein interactions; interactions of mucin glycoproteins; applications of NMR to decipher the interactions between viral proteins and the glycans on host cells; NMR methods to identify biomarkers based on the glycoprotein signals directly acquired from intact biofluids; and the applications of MD simulations and other computational methods to characterize the conformation and dynamics of glycoproteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, NMR of Glycoproteins: Methods and Protocols is a valuable resource for both new and experienced researchers who want to learn more about this important and developing field.
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Table of contents (12 protocols)
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Front Matter
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Back Matter
Editors and Affiliations
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Bibliographic Information
Book Title: NMR of Glycoproteins
Book Subtitle: Methods and Protocols
Editors: Jesús Jiménez-Barbero, Oscar Millet
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-4722-6
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025
Hardcover ISBN: 978-1-0716-4721-9Published: 02 August 2025
Softcover ISBN: 978-1-0716-4724-0Due: 16 August 2026
eBook ISBN: 978-1-0716-4722-6Published: 01 August 2025
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 210
Number of Illustrations: 8 b/w illustrations, 63 illustrations in colour
Topics: Chemistry/Food Science, general